Journal: STAR Protocols

Article Title: Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures

doi: 10.1016/j.xpro.2022.101954

Figure Lengend Snippet:

Article Snippet: PE-labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) , Acro Biosystems , Cat# FM3-HPY53; RRID: AB_2921284.

Techniques: Recombinant, Reverse Transcription, Software, Pore Size, Flow Cytometry, Transfection

Journal: Drug Design, Development and Therapy

Article Title: CTL019 (tisagenlecleucel): CAR-T therapy for relapsed and refractory B-cell acute lymphoblastic leukemia

doi: 10.2147/DDDT.S138765

Figure Lengend Snippet: Comparison of second-generation CD19 CARs in North America for relapsed B-ALL

Article Snippet: MDACC; NCI; Ziopharm; Intrexon , FMC63–IgG4 , CD3ζ , CD28 , 10 6 –10 8 /m 2 , Physician’s choice , SBT , Adults: CR: 69%, 1y PFS: 53% and OS 63% .

Techniques: Plasmid Preparation

Journal: Nature Medicine

Article Title: BCMA CAR T cells in a patient with relapsing idiopathic inflammatory myositis after initial and repeat therapy with CD19 CAR T cells

doi: 10.1038/s41591-025-03718-3

Figure Lengend Snippet: a , Serum CK levels after CD19 CAR T cell therapy and at relapse. The horizontal dotted line indicates the upper limit of normal range (<170 U per l). b , Muscle strength as indicated by MMT8 score. c , CD19 CAR + CD3 + T cell counts in blood. d , CD19 + B cell counts in blood. e – h , Counts of leukocytes ( e ); neutrophils ( f ); CD4 + T cells ( g ) and CD8 + T cells ( h ) in blood after lymphodepletion (in cells per µl). i , ELISA quantification of the humoral response to CAR (FMC63). j , Quantification of the anti-CAR T cell response in control donors and the index patient. k , Sequence of events demonstrating the onset of the anti-CAR T cell response and humoral response. Unstim, no T cell stimulation; +, stimulated T cells.

Article Snippet: Anti-FMC63 antibodies were detected using the ACROBIO Anti-CD19 (FMC63) CAR Immunogenicity ELISA Kit following kit instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Sequencing, Cell Stimulation