Journal: Cell death & disease

Article Title: Caspase-8 activation by cigarette smoke induces pro-inflammatory cell death of human macrophages exposed to lipopolysaccharide.

doi: 10.1038/s41419-023-06318-6

Figure Lengend Snippet: Fig. 2 hMDMs exposed to CSE and LPS display apoptotic hallmarks. Representative images of hMDMs treated with 1 μg/ml of LPS and 20% CSE, alone or in combination, for 24 h and stained with (A) Hoechst 33342 for nuclear morphology evaluation and (B) JC-1 dye for MMP assessment. C Quantification of Hoechst 33342 intensity expressed as mean fluorescent intensity (MFI). D Quantification of the red (485/ 590 nm)/green (485/529 nm) fluorescence intensity ratio after JC1 staining. Scale bar: 50 μm. Data are presented as mean ± SEM (N = 3 independent donors).

Article Snippet: Hoechst fluorescent nucleic acid stain (33342) was obtained from ImmunoChemistry technology, Davis, California, USA.

Techniques: Staining

Journal: bioRxiv

Article Title: Lymph node resident memory T cells retain effector capabilities by evading lung resident memory dysfunction

doi: 10.64898/2026.02.03.703569

Figure Lengend Snippet: (A) Naive CD90.1+ OT-I T cells were transferred into naive B6 mice before intranasal infection with influenza expressing SIINFEKL (PR8-OVA). Expression of CD69 and CD103 was measured 21 days post infection on extravascular cells. (B) Quantification of CD69 + CD103 + OT-I T cells. (C) Naïve CD90.1 + OT-I Nur77-GFP T cells were transferred into mice one day before intranasal PR8-OVA infection. Nur77-GFP expression was evaluated 3-14 days later by extravascular OT-I T cells. (D)Experimental setup for (E-G). Flow cytometry plot (E) of CD90.1 + OT-I T cells isolated from indicated locations. (F-G) Longitudinal labeling of cells and quantification. (H) Experimental setup for (I-J). (I) Labeling after intravenous and intraperitoneal injection of OT-I T cells or naïve endogenous T cells. (J) Expression of CD69 and CD103. Naïve= CD8 + CD90.1 - CD44 low . Each point represents a mouse and N=2-5 mice per experiment. Data are representative (A) or are pooled from at least two experiments (n=2–4/group), except for day 113 in (B), which reflect a single experiment.

Article Snippet: Measurements were obtained in Flow Cytometry (FACS) Staining Buffer (Rockland, MB-086-0500) at room temperature for about 30 minutes per sample and channels were flushed with buffer in between samples.

Techniques: Infection, Expressing, Flow Cytometry, Isolation, Labeling, Injection

Journal: bioRxiv

Article Title: Lymph node resident memory T cells retain effector capabilities by evading lung resident memory dysfunction

doi: 10.64898/2026.02.03.703569

Figure Lengend Snippet: (A)Chromatin accessibility with gene expression (right) and linked chromatin peaks (bottom) at the Gzmb locus. (B) Protein expression of GranzymeB as measured by flow cytometry. (C-D) Same as (A-B) but for the Pdcd1 locus. Protein expression on OT-I T cells is 49 days post infection where each dot represents one mouse. (J and L) Data are from one experiment and representative of at least two experiments with N=3. (E-F) Surface expression of PD-1 and TIM3 at 7 days post infection. (G) Transcription factor motif footprint analysis from dataset. (H) Inferred eRegulons identified by SCENIC+ across memory T cells after influenza infection. (I-K) Legend (left) for network plots illustrating inferred transcription factor relationships to target genes. Transcription factor and gene relationship is denoted by color of line represents activation (red) or repression (blue).

Article Snippet: Measurements were obtained in Flow Cytometry (FACS) Staining Buffer (Rockland, MB-086-0500) at room temperature for about 30 minutes per sample and channels were flushed with buffer in between samples.

Techniques: Gene Expression, Expressing, Flow Cytometry, Infection, Activation Assay

Journal: Scandinavian journal of immunology

Article Title: Mucosal-associated invariant T cells modulate innate immune cells and inhibit colon cancer growth.

doi: 10.1111/sji.13391

Figure Lengend Snippet: FIGURE 3 Activation of MAIT cells enhance killing against colon cancer cells. (A) Experimental setup of human whole leukocyte isolation and killing assay co-culture with COLO 205. (B) Percent specific lysis of COLO 205 co-cultured with human whole leukocytes with or without 5-A-RU/MGO stimulation using a 4-h Calcein Release Assay. (C) Frequency of CFSE+ cells after overnight co-culture of human whole leukocytes with COLO 205 with or without 5-A-RU/MGO stimulation for 16 h by flow cytometry. Each dot represents a sample from an individual healthy donor and data were collected from two independent experiments. Figure created using BioRender.com. *p < 0.05 by Wilcoxon ranked test.

Article Snippet: For flow cytometry- based killing assay, 100,000 CFSE- stained (Cat. #: 6162, Immunochemistry Technologies, Davis, California, CA, USA) COLO 205 was co- cultured with 106 human whole leukocytes for 16 h and the frequency of CFSE+ cells was analysed.

Techniques: Activation Assay, Isolation, Co-Culture Assay, Lysis, Cell Culture, Release Assay, Flow Cytometry