Journal: Stem Cell Research & Therapy

Article Title: NT3 P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model

doi: 10.1186/s13287-019-1428-1

Figure Lengend Snippet: NT3 P75-2 overexpression enhances the growth of BMSCs in vitro. a Representative images of green fluorescence protein (GFP) in BMSCs after infecting with lentiviruses (GFP, NT3, or NT3 P75-2 ) at 3 days post-infection ( n = 3). b The counting data of percent of GFP-positive BMSCs in different groups. c NT3 expression levels in BMSCs after infecting with lentiviruses (GFP, NT3, or NT3 P75-2 ) were analyzed by western blot, and the statistical data was also presented ( # P < 0.05, *** P < 0.001 by one-way ANOVA followed by Bonferroni’s multiple comparison test, n = 3). d NT3 concentrations of cellular supernatant in BMSCs after infecting with lentiviruses (GFP, NT3, and NT3 P75-2 ) were assessed by ELISA ( # P < 0.05, *** P < 0.001 by one-way ANOVA followed by Bonferroni’s multiple comparison test, n = 6). e The cell growth of BMSCs after infecting with lentiviruses (GFP, NT3, or NT3 P75-2 ) was measured by CCK8 assays ( # P < 0.05, ### P < 0.001, ** P < 0.01, *** P < 0.001 by two-way ANOVA followed by Bonferroni’s multiple comparison test, n = 6)

Article Snippet: Infection efficiency was checked by detecting the GFP fluorescence (1:500, Santa Cruz Biotechnology) via immunofluorescent staining at 3 days post-infection.

Techniques: Over Expression, In Vitro, Fluorescence, Infection, Expressing, Western Blot, Comparison, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Bromine radical as a visible-light-mediated polarity-reversal catalyst

doi: 10.1016/j.isci.2021.102693

Figure Lengend Snippet:

Article Snippet: thin layer chromatography using TLC silica gel plates with fluorescent indicator (λ = 254 nm) , Merck-Schuchardt , https://merckmillipore.com/.

Techniques: Recombinant, Software, Thin Layer Chromatography

Journal: iScience

Article Title: Alzheimer's Aβ assembly binds sodium pump and blocks endothelial NOS activity via ROS-PKC pathway in brain vascular endothelial cells

doi: 10.1016/j.isci.2021.102936

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Article Snippet: The cells were washed twice with HBSS containing Mg + and Ca 2+ (HBSS, 09735-75, Nacalai tesque) and were loaded with a fluorescent NO indicator DAF-FM diacetate (SKM423741, Sekisui Medical, Tokyo, Japan), according to the manufacturer’s protocol.

Techniques: Recombinant, Avidin-Biotin Assay, Synthesized, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Derivative Assay, Software, Light Microscopy, Laser-Scanning Microscopy, Imaging, Cytometry

Journal: Immunity & Ageing : I & A

Article Title: Immunosenescence in persons with spinal cord injury in relation to urinary tract infections -a cross-sectional study-

doi: 10.1186/s12979-017-0103-6

Figure Lengend Snippet: In vitro T-cell exhaustion and proliferation. The top row shows the expression of the surface exhaustion marker “killer cell lectin-like receptor G1” (KLRG) as determined by flow cytometry for CD4 ( a ) and CD8 ( b ) T-cells ( n = 41, 42, 42 and 41). The bottom four graphs show the results of a 7 days in vitro stimulation of PBL with the mitogen PHA. Middle row depicts the percent of CD4 ( c ) and CD8 ( d ) T-cells which divided at least once or more ( n = 41, 39, 35 and 39). Bottom row represents the results for the average number of cell divisions for CD4 ( e ) and CD8 ( f ) T-cells after a 7 day in vitro assay with PHA ( n = 41, 39, 35 and 39). The horizontal mark indicates the median of each group: able bodied controls <60 years (yCtr), SCI < 60 years (ySCI), able bodied controls ≥60 years (oCtr) and SCI ≥ 60 years (oSCI). Significant differences are indicated as *: p < 0.05 and *** p < 0.001

Article Snippet: The thawed PBL, allowed to rest overnight as described above, were used for an in vitro proliferation assay using the fluorescent cell proliferation indicator CytoTell green (AAT Bioquest; LuBioScience), according to the manufactures protocol (20 min incubation at room temperature in 1:600 diluted dye).

Techniques: In Vitro, Expressing, Marker, Flow Cytometry