Journal: Scientific Reports

Article Title: An investigation on the structural, morphological, optical, and antibacterial activity of Sr:CuS nanostructures

doi: 10.1038/s41598-024-73701-4

Figure Lengend Snippet: TXRF quantitative analysis of CuS and Sr-doped CuS nanoparticles.

Article Snippet: The elemental analysis was performed using a total reflection X-ray fluorescence (TXRF) spectrum (Bruker S8 TIGER-German).

Techniques:

Journal: Plant physiology

Article Title: EXTRA LARGE G-PROTEIN2 mediates cell death and hyperimmunity in the chitin elicitor receptor kinase 1-4 mutant.

doi: 10.1093/plphys/kiac214

Figure Lengend Snippet: Figure 8 XLG2 functions in cerk1-4 cell death signaling together with PUB2. A, Venus-XLG2 and Venus-XLG2E293K were isolated from stably trans- formed Arabidopsis lines with anti-GFP nanobody beads. The samples were analyzed by LC–MS/MS and PSMs corresponding to XLG2/XLG2E293K, PUB4 and PUB2 were counted. Data are presented as average ±SD of three independent transgenic lines. Untransformed xlg2-2 served as a negative control. B, Western blot of the indicated lines with a specific XLG2 antibody. The experiment was done three times. C, pub2-1 and pub4-3 were crossed with cerk1-4. Single and double mutants as well as the background accessions Col-0 and Col-3 gl1 were inoculated with Blumeria graminis f. sp. hordei (Bgh) and analyzed at 7 dpi. Images of representative plants are shown. Inoculation experiments were performed twice with Bgh and twice with E. cruciferarum. D, 7 dpi Bgh infection, six whole rosettes per genotype were pooled and used for Western blots with a PR1-specific anti- body. CBB, Coomassie brilliant blue-stained membrane. PR1 accumulation was tested twice with Bgh and twice with E. cruciferarum.

Article Snippet: For GFP pull-downs, standard protein extracts were prepared (Petutschnig et al., 2010) and the target protein was isolated with either Chromotek GFP trap beads (mass spectrometry experiments) or Nanotag GFP selector beads (Co-IP experiments).

Techniques: Isolation, Stable Transfection, Liquid Chromatography with Mass Spectroscopy, Transgenic Assay, Negative Control, Western Blot, Infection, Staining, Membrane

Journal: Cell Reports Methods

Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

doi: 10.1016/j.crmeth.2026.101330

Figure Lengend Snippet: Assessing spectral resolvability of metabolic probes for multiparametric cytometry (A) Experimental workflow for evaluating probe co-detection. (B and D) Representative density plots demonstrating spectral overlap for pairwise probe combinations within the (B) FITC and (D) PE channel. (C and E) Complementary validation matrices showing computational spectral similarity scores from FluoroFinder (lower left quadrant) versus empirical resolvability determined by unmixing (upper right quadrant). Gray indicates non-resolvable pairs; colored tiles indicate resolvable pairs (C, green; E, red).

Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

Techniques: Cytometry, Biomarker Discovery

Journal: Cell Reports Methods

Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

doi: 10.1016/j.crmeth.2026.101330

Figure Lengend Snippet: Validation framework for spectral co-resolvability of metabolic probes and fluorophore conjugates (A) Experimental workflow for co-detection assessment. (B, D, and F) Representative flow cytometry density plots demonstrating pairwise mixing between metabolic probes and fluorophores in the (B) FITC/AF488, (D) PE, and (F) APC/AF647 channel. (C, E, and G) Validation matrices per channel: upper, computational spectral similarity by FluoroFinder; lower, empirically determined resolvability. Gray tiles indicate non-resolvable pairs; colored tiles confirm resolvable pairs. (C) FITC/AF488, green; (E) PE, red; and (G) APC/AF647, blue.

Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

Techniques: Biomarker Discovery, Flow Cytometry

Journal: Cell Reports Methods

Article Title: Application of spectral flow cytometry for comprehensive detection of immune metabolism in patient-derived microsamples

doi: 10.1016/j.crmeth.2026.101330

Figure Lengend Snippet: Validation of three metabolic probes for simultaneous assessment of mitochondrial activity and oxidative stress (A) Representative flow cytometry density plots demonstrating spectral resolution of co-stained probes. (B) Validation matrix: lower left quadrant, computational spectral similarity by FluoroFinder; upper right quadrant, empirical resolvability determination (red: resolvable pairs). (C) Mean fluorescence intensity (MFI; mean ± SD) of individual probes in control versus rotenone/antimycin A (Rot/AA)-treated groups ( n = 6). (D) Correlation analysis of MFI between single-stain and multiplexed conditions across probes (Spearman’s r). (E) MFI of probes (mean ± SD) in control versus EZH2-knockdown (EZH2-sh) groups under single and multiplexed staining. ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001.

Article Snippet: Cells were washed and then acquired by full spectrum flow cytometry (SONY ID7000).

Techniques: Biomarker Discovery, Activity Assay, Flow Cytometry, Staining, Fluorescence, Control, Knockdown

Journal: Indian Journal of Ophthalmology

Article Title: A short-term chick embryo in vivo xenograft model to study retinoblastoma cancer stem cells

doi: 10.4103/ijo.IJO_2348_21

Figure Lengend Snippet: Evidence of Rb spontaneous metastasis in different parts of chick embryo analyzed using in vivo IVIS spectral imaging. Representative image of in vivo fluorescence observed in chick embryo transplanted with (a) CM-Dil Y79 CD133 - cells, (b) CM-Dil Y79 CD133 + cells, (d) eGFP Y79 CD133 - cells, and (e) eGFP Y79 CD133 + cells. Quantification of fluorescence intensity within the chick embryo revealed that CD133 - cells had increased spontaneous metastasis when compared to CD133 + cells (**P = 0.0077, 0.0017)

Article Snippet: The whole embryos were analyzed using the IVIS Spectrum in vivo optical imaging system (PerkinElmer) to track the presence of labeled tumor cells.

Techniques: In Vivo, Imaging, Fluorescence