Journal: Journal of Pathology Informatics

Article Title: New European Union Regulations Related to Whole Slide Image Scanners and Image Analysis Software

doi: 10.4103/jpi.jpi_33_18

Figure Lengend Snippet: Digital pathology solutions that have received CE mark as compliant with directive 98/79/EC in vitro diagnostic directive*

Article Snippet: 3DHistech , Pannoramic 250 Flash II, Pannoramic SCAN, Pannoramic MIDI, Pannoramic DESK , Brightfield slide scanner.

Techniques: In Vitro, Diagnostic Assay, Software, Biomarker Discovery, Imaging, Fluorescence, Labeling

Journal: Journal of Nanobiotechnology

Article Title: Real-world of Limosilactobacillus reuteri in mitigation of acute experimental colitis

doi: 10.1186/s12951-025-03158-8

Figure Lengend Snippet: L. reuteri- CMVs promote tight junction proteins in vitro. (A , B ) Dil-labeled L. reuteri -CMVs are internalized by HT-29 and Caco2 cells, and the Dil fluorescence intensity increased with prolonged co-incubation time. ( C ) The mRNA expression levels of ZO-1 and Cdh1 in HT-29 and Caco2 cells. ( D-F ) The protein levels of ZO-1 and Cdh1 in HT-29 and Caco2 cells using Western blot and IF analysis. Data are mean ± SEM ( n = 3). * p < 0.05, ** p < 0.01

Article Snippet: Images were acquired using a fluorescence scanner (Pannoramic MIDI, 3DHISTECH, Hungary).

Techniques: In Vitro, Labeling, Fluorescence, Incubation, Expressing, Western Blot

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Biodistributions of intact monoclonal antibodies and fragments of BLCA-38, a new prostate cancer directed antibody

doi: 10.1007/s00262-003-0460-1

Figure Lengend Snippet: A Flow cytometric profiles of human prostate cancer cell lines grown in vitro and stained with BLCA-38 or J591 (10 μg). (a) BLCA-38 MAb expression on DU-145 cell (mean fluorescence intensity [MFI]: trypsin, 28.4; MFI EDTA, 185.8); (b) J591 expression on LNCaP-LN3 cells (MFI trypsin, 180.5; MFI EDTA, 496.3). Negative control (second antibody only), cells harvested in trypsin (MFI DU-145, 4.6; MFI LNCaP-LN3, 5.4), and cells harvested in EDTA/PBS (MFI DU-145, 4.7; MFI LNCaP-LN3, 5.4). B. Tumour xenografts grown s.c. in nude mice. DU-145 xenografts were formalin-fixed and paraffin-embedded, and LNCaP-LN3 tumours were fresh-frozen in OCT by standard methods. Xenografts were stained for expression by indirect immunoperoxidase staining with BLCA-38 MAbs (for DU-145 xenografts) or J591 MAbs (for LNCaP-LN3 xenografts) . DU-145 xenografts first underwent antigen retrieval in a 1,200 W microwave oven at maximum power for 3 min then at 95°C for another 10 min, followed by cooling at room temperature for 15 min. (a) Photomicrograph showing BLCA-38 expression in DU-145 xenograft; (b) Photomicrograph of DU-145 xenograft stained with isotype control antibody showing negative staining; (c) Photomicrograph showing positive J591 staining of LNCaP-LN3 xenograft; (d) Photomicrograph of LNCaP-LN3 tissue stained with isotype control antibody

Article Snippet: Surface fluoresescence was assessed in a Fluorescence Activated Cell Scanner (FACScan; Becton Dickinson, Mountain View, CA).

Techniques: In Vitro, Staining, Expressing, Fluorescence, Negative Control, Immunoperoxidase Staining, Negative Staining