flt Search Results


antihuman vegfr1 antibody  (R&D Systems)
99
R&D Systems antihuman vegfr1 antibody
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Antihuman Vegfr1 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti vegfr 3 antibody  (R&D Systems)
91
R&D Systems mouse anti vegfr 3 antibody
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Mouse Anti Vegfr 3 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human flt 3 ligand quantikine elisa kit  (R&D Systems)
94
R&D Systems human flt 3 ligand quantikine elisa kit
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Human Flt 3 Ligand Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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recombinantmousevegfr3  (R&D Systems)
93
R&D Systems recombinantmousevegfr3
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Recombinantmousevegfr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse svegfr1 duoset elisa kits  (R&D Systems)
94
R&D Systems mouse svegfr1 duoset elisa kits
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Mouse Svegfr1 Duoset Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti vegfr 3  (Proteintech)
93
Proteintech anti vegfr 3
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Anti Vegfr 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vefgr3  (R&D Systems)
94
R&D Systems vefgr3
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Vefgr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biotinylated antibody  (R&D Systems)
92
R&D Systems biotinylated antibody
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Biotinylated Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human fgfr1 rg202080 origene  (OriGene)
92
OriGene human fgfr1 rg202080 origene
Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of <t>VEGFR1</t> (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.
Human Fgfr1 Rg202080 Origene, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti flt1 antibody  (Proteintech)
94
Proteintech anti flt1 antibody
Fig. 10 Diagram of the hypothesis mechanism of tsRNA-3043a facilitates POF by inhibiting <t>FLT1</t>
Anti Flt1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human flt3l  (R&D Systems)
94
R&D Systems human flt3l
Fig. 10 Diagram of the hypothesis mechanism of tsRNA-3043a facilitates POF by inhibiting <t>FLT1</t>
Human Flt3l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vegfr3  (R&D Systems)
94
R&D Systems vegfr3
Fig. 10 Diagram of the hypothesis mechanism of tsRNA-3043a facilitates POF by inhibiting <t>FLT1</t>
Vegfr3, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.

Journal: Molecular Cancer

Article Title: Tylophorine, a phenanthraindolizidine alkaloid isolated from Tylophora indica exerts antiangiogenic and antitumor activity by targeting vascular endothelial growth factor receptor 2–mediated angiogenesis

doi: 10.1186/1476-4598-12-82

Figure Lengend Snippet: Tylophorine inhibits VEGFR2 binding with VEGF and attenuated VEGFR2 tyrosine kinase activity. (A) Effect of tylophorine on the binding of VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1) to immobilized VEGF. Data were presented as means ± SEM, n = 6. **p < 0.01; ***p < 0.001 compared with control. (B) Inhibition of VEGFR2 kinase activity by tylophorine was analyzed using an in vitro HTScan® VEGF receptor 2 kinase kit (Cell Signaling Technology, Danvers, MA, USA) combined with colorimetric ELISA detection according to the manufacturer’s instructions. The reaction processed with DMSO (0.1%) served as a vehicle control. Data were expressed as percentages of the vehicle control. (C) Western blot analyses of effect of tylophorine on phosphorylation of VEGFR2. HUVECs were pre-treated with tylophorine followed by the stimulation with 50 ng/mL of VEGF for 2 min. Data were presented as means ± SEM, n = 6. (D) Quantitative densitometry of VEGFR2 phosphorylation is shown as percentage (%) of control. Data were presented as means ± SEM, n = 6. ## P < 0.01 VEGF-treated group versus no VEGF-treated group; **p < 0.01; ***p < 0.001 versus VEGF-stimulated group.

Article Snippet: Antihuman VEGFR1 antibody (AF321, R&D Systems) and antihuman VEGFR2 antibody (MAB3572, R&D Systems) were used as positive control for VEGFR1 and VEGFR2 respectively (data not shown).

Techniques: Binding Assay, Activity Assay, Control, Inhibition, In Vitro, Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics

Fig. 10 Diagram of the hypothesis mechanism of tsRNA-3043a facilitates POF by inhibiting FLT1

Journal: Journal of molecular histology

Article Title: tsRNA-3043a intensifies apoptosis and senescence of ovarian granulosa cells to drive premature ovarian failure by targeting FLT1.

doi: 10.1007/s10735-024-10256-8

Figure Lengend Snippet: Fig. 10 Diagram of the hypothesis mechanism of tsRNA-3043a facilitates POF by inhibiting FLT1

Article Snippet: The sections were blocked with 5% BSA and incubated with the anti-FLT1 antibody (1:200, 13687-1-AP, Proteintech) overnight at 4°C.

Techniques: