Journal: Journal of Applied Genetics

Article Title: Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

doi: 10.1007/s13353-021-00641-4

Figure Lengend Snippet: Phenotypic analysis of cells by flow cytometry. Number of positive cells for the tested marker expressed in %

Article Snippet: Following centrifugation, the cells were resuspended in Flow Cytometry Fixation/Permeabilization Buffer I (R&D Systems, Minneapolis, MN, USA) and incubated at 2–8 °C for 30 min. After incubation, the cells were centrifuged at 587 × g for 5 min and resuspended to a final density of 10 6 cells in 200 μl of Flow Cytometry Permeabilization/Wash Buffer I (cat. no. FC005, R&D Systems, Minneapolis, MN, USA).

Techniques: Flow Cytometry, Marker