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Danaher Inc
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Journal:
Article Title: Molecular and pharmacological characterization of a functional tachykinin NK 3 receptor cloned from the rabbit iris sphincter muscle
doi: 10.1038/sj.bjp.0702854
Figure Lengend Snippet: Concentration effect curves to senktide (n=5), [MePhe7]-NKB (n=5), NKB (n=4), NKA (n=4) and SP (n=4) in CHO-K1 cells transiently expressing the rabbit NK3 receptor. Increases in intracellular calcium induced by each agonist were measured using FLIPR. Results are expressed as a percentage of the maximum response and data are mean with vertical lines showing s.e.mean.
Article Snippet: The concentration of drug inhibiting specific [ 125 I]-[MePhe 7 ]-NKB binding by 50% (IC 50 ) was determined by iterative curve fitting ( Bowen & Jerman, 1995 ). pK i values (−log of the inhibition constant) for receptor binding were then calculated from the IC 50 values as described by Cheng & Prusoff (1973) using a K d value of 0.3 n M . Measurement of intracellular calcium using
Techniques: Concentration Assay, Expressing