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Image Search Results
Journal: Scientific Reports
Article Title: Real-time Brain Tumor imaging with endogenous fluorophores: a diagnosis proof-of-concept study on fresh human samples
doi: 10.1038/s41598-018-33134-2
Figure Lengend Snippet: ( a ) 3D scatter plot of three quantitative tissue indicators: the redox ratio, fluorescence lifetime, and SHG signal averaged for each tissue subgroup with a Gaussian ellipsoid fit, ( b ) box plot of the average lifetimes, ( c ) bar graph of the redox ratios for each tissue type with the errors corresponding to the standard deviations across all measurements, and ( d ) overlaid TPEF and SHG intensity images.
Article Snippet: The microscope integrated a
Techniques: Fluorescence
Journal: bioRxiv
Article Title: Multiplexed in vivo imaging with fluorescence lifetime modulating tags
doi: 10.1101/2024.04.12.589181
Figure Lengend Snippet: ( a ) Principle of fluorescence lifetime multiplexing of FAST variants. FAST:fluorogen assemblies with similar spectral properties but different lifetime signatures can be distinguished analyzing their lifetimes. ( b-e ) Intensity-weighted average lifetime distributions of shortT550, midT550 and longT550 in HEK293T cells ( b ), HeLa cells ( c ) and U2OS cells ( d ). N cells from two biological replicates were analyzed (the number N of analyzed cells is given in ( j )). Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the two biological replicates. ( e ) Means of intensity-weighted average fluorescence lifetimes of shortT550, midT550 and longT550 in the three cell lines. ( f-i ) Intensity-weighted average lifetime distributions of shortT560, midT560 and longT560 in HEK293T cells ( f ), HeLa cells ( g ) and U2OS cells ( h ). N cells from two biological replicates were analyzed (the number N of analyzed cells is given in ( j )). Each cell is color-coded according to the biological replicate it came from. The solid circles correspond to the mean of each biological replicate. The black line represents the mean ± SD of the two biological replicates. ( i ) Means of intensity-weighted average fluorescence lifetimes of shortT560, midT560 and longT560 in the three cell lines. ( j ) Number of cells for each set of experiments. ( k,l ) Photophysical properties of FAST variants with HBR-2,5DM ( k ) and HBR-3,5DM ( l ). Each dot corresponds to one FAST:fluorogen assembly according to their radiative and non-radiative decay constant values, and is scaled to the FQY of the assembly.
Article Snippet: FLIM images were acquired on a Zeiss LSM 980 confocal system coupled with
Techniques: Fluorescence, Multiplexing