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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Analytical Criticalities Associated to Different Immunological Methods for Serum Free Light Chain Detection in Plasma Cell Dyscrasias: A Description of Particular Clinical Cases
doi: 10.3390/ijms18040804
Figure Lengend Snippet: First clinical case: ( A ) Agarose gel electrophoresis of serum proteins, performed with semi-automatic analyzer Hydrasys 2 (Sebia), shows a normal profile; ( B ) High resolution electrophoresis performed on agarose gel in urine (U) and serum (S) samples of patient. In both matrices M-proteins were detected; ( C , D ) Immunofixation of serum ( C ) and urine ( D ). The figures show two monoclonal λ-free light chains proteins. ELP: fixative for electrophoretically separated proteins; G: IFE with antiserum anti-γ; A: with antiserum anti-α; M: with antiserum anti-μ; K: with antiserum anti-κ; L: with antiserum anti-λ.
Article Snippet: Currently available methods for sFLC quantification are the FreeliteTM assay (The Binding Site) and the
Techniques: Agarose Gel Electrophoresis, Electrophoresis
Journal: International Journal of Molecular Sciences
Article Title: Analytical Criticalities Associated to Different Immunological Methods for Serum Free Light Chain Detection in Plasma Cell Dyscrasias: A Description of Particular Clinical Cases
doi: 10.3390/ijms18040804
Figure Lengend Snippet: The measurement of sFLC using N-Latex FLC and Freelite™ on serum in the three clinical case.
Article Snippet: Currently available methods for sFLC quantification are the FreeliteTM assay (The Binding Site) and the
Techniques:
Journal: International Journal of Molecular Sciences
Article Title: Analytical Criticalities Associated to Different Immunological Methods for Serum Free Light Chain Detection in Plasma Cell Dyscrasias: A Description of Particular Clinical Cases
doi: 10.3390/ijms18040804
Figure Lengend Snippet: First clinical case: ( A ) Serum immunofixation of patients with anti-IgD, anti-IgE, and anti-λ free light chains antisera, which confirms the presence of an M-protein consisting of free light chains λ. EP: electrophoretic migration; IgD: IFE with antiserum δ; IgE: IFE with antiserum ε; Ktot: IFE with antiserum anti-κ; λ tot: IFE with anti-λ antiserum; λ free: IFE with specific antiserum anti-λ free; ( B ) Immunoblotting of serum (S) and urine (U) of the patient. The monoclonal protein is evident in both biological matrices; ( C ) SDS-PAGE is used for the separation of proteins based on their molecular weight. The bands are recovered after electrophoresis on agarose gel high-resolution. The figure shows the presence of proteins with MW of 22,000 and 44,000 Da, corresponding to monomers and dimers of λ free light chains. M: proteins PM-known index; Ctrl: control serum of patient with known monoclonal protein λ free light chain; U: urine of patient; S: serum of patient.
Article Snippet: Currently available methods for sFLC quantification are the FreeliteTM assay (The Binding Site) and the
Techniques: Migration, Western Blot, SDS Page, Molecular Weight, Electrophoresis, Agarose Gel Electrophoresis, Control
Journal: Antimicrobial Agents and Chemotherapy
Article Title: Molecular Confirmation of the Linkage between the Rhizopus oryzae CYP51A Gene Coding Region and Its Intrinsic Voriconazole and Fluconazole Resistance
doi: 10.1128/AAC.00224-18
Figure Lengend Snippet: Disk diffusion susceptibility testing using fluconazole (FLC) and voriconazole (VRC) paper disks for LMDM-31 (parental strain akuBKU80Δ) (31), LMDM-32 (recipient strain akuBKU80Δ CYP51AΔ) (12), R. oryzae ATCC 11886, and A. fumigatus chimera LMDM-1229 (akuBKU80Δ RoCYP51A).
Article Snippet: The
Techniques: Diffusion-based Assay