flag m2 moab Search Results


rat α flag tag  (Novus Biologicals)
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monoclonal m2 anti-flag antibody  (Agilent technologies)
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Agilent technologies monoclonal m2 anti-flag antibody

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anti-flag m2 mab  (Agilent technologies)
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mouse anti-flag m2 amersham ecl  (Amersham Life Sciences Inc)
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monoclonal antibody against flag (m2  (Babco Inc)
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Babco Inc monoclonal antibody against flag (m2

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biotinylated anti-flag monoclonal antibody m2  (Amersham Life Sciences Inc)
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antibody-agarose  (Millipore)
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Millipore antibody-agarose

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sc 8017 mouse monoclonal flag m2  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology sc 8017 mouse monoclonal flag m2

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anti-flag mab  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti-flag mab

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mouse monoclonal -flag antibody  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology mouse monoclonal -flag antibody

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monoclonal anti flag m2 antibody  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc monoclonal anti flag m2 antibody
Figure 4. PKG (protein kinase G) 1α C42 oxidation determines PDE5 activation and sildenafil (SIL) efficacy in iso- lated myocytes. All bar graphs are shown as mean±SD. A, PDE5 activity in neonatal rat ventricular myocytes (NRVMs) exposed to cyclic GMP (cGMP)+H2O2; P<0.001 for genotype effect; P=0.003 for interaction of cGMP+H2O2×genotype by 2-way ANOVA; *P<0.001 vs control (CON). B, PKG activity before and after H2O2+cGMP; P<0.0001 for effect of cGMP+H2O2; P>0.5 for interaction of cGMP+H2O2×genotype by 2-way ANOVA. C, PDE5 Ser92 phosphorylation and total protein level in adult mouse cardiomyo- cytes from WT (wild type) and KI hearts. Example gel and summary data are shown. Cells were stimulated with cGMP+SIL with or without addition of PKG1α inhibitor DT3; *P<0.001 vs other groups. D, Nppb/Gapdh expression in response to ET (endothelin)-1 in NRVMs expressing PDE5WT, PDE5S92D, or PDE5S92A. *P<0.001 vs WT and S92A responses, by 1-way ANOVA. E, Nonreducing gel for PKG-PDE5 interaction in NRVMs expressing <t>FLAG-PKG1αWT</t> or FLAG-PKG1αC42S. Pull-down used mouse <t>monoclonal</t> <t>anti-FLAG</t> antibody and gels probed for PDE5A or FLAG to detect dimer/monomer ratio of PKG1α forms. F, Summary data for studies as in (E). Top, The percentage of dimeric PKG increases after H2O2±cGMP in WT expressing cells; *P<0.0001 vs CON. Bottom, Coprecipitation of PDE5 and PKG1α: P<0.001 for effect of H2O2±cGMP, P=0.54 for stimulus genotype interaction; †P=0.009; ‡P=0.06 vs CON.
Monoclonal Anti Flag M2 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-flag m2 mouse monoclonal antibody  (Millipore)
90
Millipore anti-flag m2 mouse monoclonal antibody
Figure 4. PKG (protein kinase G) 1α C42 oxidation determines PDE5 activation and sildenafil (SIL) efficacy in iso- lated myocytes. All bar graphs are shown as mean±SD. A, PDE5 activity in neonatal rat ventricular myocytes (NRVMs) exposed to cyclic GMP (cGMP)+H2O2; P<0.001 for genotype effect; P=0.003 for interaction of cGMP+H2O2×genotype by 2-way ANOVA; *P<0.001 vs control (CON). B, PKG activity before and after H2O2+cGMP; P<0.0001 for effect of cGMP+H2O2; P>0.5 for interaction of cGMP+H2O2×genotype by 2-way ANOVA. C, PDE5 Ser92 phosphorylation and total protein level in adult mouse cardiomyo- cytes from WT (wild type) and KI hearts. Example gel and summary data are shown. Cells were stimulated with cGMP+SIL with or without addition of PKG1α inhibitor DT3; *P<0.001 vs other groups. D, Nppb/Gapdh expression in response to ET (endothelin)-1 in NRVMs expressing PDE5WT, PDE5S92D, or PDE5S92A. *P<0.001 vs WT and S92A responses, by 1-way ANOVA. E, Nonreducing gel for PKG-PDE5 interaction in NRVMs expressing <t>FLAG-PKG1αWT</t> or FLAG-PKG1αC42S. Pull-down used mouse <t>monoclonal</t> <t>anti-FLAG</t> antibody and gels probed for PDE5A or FLAG to detect dimer/monomer ratio of PKG1α forms. F, Summary data for studies as in (E). Top, The percentage of dimeric PKG increases after H2O2±cGMP in WT expressing cells; *P<0.0001 vs CON. Bottom, Coprecipitation of PDE5 and PKG1α: P<0.001 for effect of H2O2±cGMP, P=0.54 for stimulus genotype interaction; †P=0.009; ‡P=0.06 vs CON.
Anti Flag M2 Mouse Monoclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: eLife

Article Title: Cell types and neuronal circuitry underlying female aggression in Drosophila

doi: 10.7554/eLife.58942

Figure Lengend Snippet:

Article Snippet: Antibody , Rat α-FLAG Tag (DYKDDDDK Epitope tag) monoclonal , Novus Biologicals , Cat #: NBP1-06712 , Dilution (1:200).

Techniques: Electron Microscopy

Figure 4. PKG (protein kinase G) 1α C42 oxidation determines PDE5 activation and sildenafil (SIL) efficacy in iso- lated myocytes. All bar graphs are shown as mean±SD. A, PDE5 activity in neonatal rat ventricular myocytes (NRVMs) exposed to cyclic GMP (cGMP)+H2O2; P<0.001 for genotype effect; P=0.003 for interaction of cGMP+H2O2×genotype by 2-way ANOVA; *P<0.001 vs control (CON). B, PKG activity before and after H2O2+cGMP; P<0.0001 for effect of cGMP+H2O2; P>0.5 for interaction of cGMP+H2O2×genotype by 2-way ANOVA. C, PDE5 Ser92 phosphorylation and total protein level in adult mouse cardiomyo- cytes from WT (wild type) and KI hearts. Example gel and summary data are shown. Cells were stimulated with cGMP+SIL with or without addition of PKG1α inhibitor DT3; *P<0.001 vs other groups. D, Nppb/Gapdh expression in response to ET (endothelin)-1 in NRVMs expressing PDE5WT, PDE5S92D, or PDE5S92A. *P<0.001 vs WT and S92A responses, by 1-way ANOVA. E, Nonreducing gel for PKG-PDE5 interaction in NRVMs expressing FLAG-PKG1αWT or FLAG-PKG1αC42S. Pull-down used mouse monoclonal anti-FLAG antibody and gels probed for PDE5A or FLAG to detect dimer/monomer ratio of PKG1α forms. F, Summary data for studies as in (E). Top, The percentage of dimeric PKG increases after H2O2±cGMP in WT expressing cells; *P<0.0001 vs CON. Bottom, Coprecipitation of PDE5 and PKG1α: P<0.001 for effect of H2O2±cGMP, P=0.54 for stimulus genotype interaction; †P=0.009; ‡P=0.06 vs CON.

Journal: Circulation: Heart Failure

Article Title: Prevention of PKG-1α Oxidation Suppresses Antihypertrophic/Antifibrotic Effects From PDE5 Inhibition but not sGC Stimulation

doi: 10.1161/circheartfailure.117.004740

Figure Lengend Snippet: Figure 4. PKG (protein kinase G) 1α C42 oxidation determines PDE5 activation and sildenafil (SIL) efficacy in iso- lated myocytes. All bar graphs are shown as mean±SD. A, PDE5 activity in neonatal rat ventricular myocytes (NRVMs) exposed to cyclic GMP (cGMP)+H2O2; P<0.001 for genotype effect; P=0.003 for interaction of cGMP+H2O2×genotype by 2-way ANOVA; *P<0.001 vs control (CON). B, PKG activity before and after H2O2+cGMP; P<0.0001 for effect of cGMP+H2O2; P>0.5 for interaction of cGMP+H2O2×genotype by 2-way ANOVA. C, PDE5 Ser92 phosphorylation and total protein level in adult mouse cardiomyo- cytes from WT (wild type) and KI hearts. Example gel and summary data are shown. Cells were stimulated with cGMP+SIL with or without addition of PKG1α inhibitor DT3; *P<0.001 vs other groups. D, Nppb/Gapdh expression in response to ET (endothelin)-1 in NRVMs expressing PDE5WT, PDE5S92D, or PDE5S92A. *P<0.001 vs WT and S92A responses, by 1-way ANOVA. E, Nonreducing gel for PKG-PDE5 interaction in NRVMs expressing FLAG-PKG1αWT or FLAG-PKG1αC42S. Pull-down used mouse monoclonal anti-FLAG antibody and gels probed for PDE5A or FLAG to detect dimer/monomer ratio of PKG1α forms. F, Summary data for studies as in (E). Top, The percentage of dimeric PKG increases after H2O2±cGMP in WT expressing cells; *P<0.0001 vs CON. Bottom, Coprecipitation of PDE5 and PKG1α: P<0.001 for effect of H2O2±cGMP, P=0.54 for stimulus genotype interaction; †P=0.009; ‡P=0.06 vs CON.

Article Snippet: All protein extracts were run on Novex Tris-Glycine Gels (Life Technologies), then blotted onto 3 nitrocellulose membranes, and probed with each specific primary antibody such as PKG1α (#13511, Cell Signaling Technology), monoclonal anti-FLAG M2 antibody (#14793, Cell Signaling Technology), phospho Ser92 PDE5A (GTX36930, GeneTex), or total-PDE5A (#2395, Cell Signaling Technology).

Techniques: Activation Assay, Activity Assay, Control, Phospho-proteomics, Expressing