Journal: Journal of Photopolymer Science and Technology

Article Title: Implantable Microfluidic Device with Hydrogel Permeable Membrane for Delivering Chemical Compounds and Imaging Neural Cells in Living Mice

doi: 10.2494/photopolymer.29.513

Figure Lengend Snippet: Figure 1. Design of an implantable microfluidic device with hydrogel permeable membrane. (a) Schematic illustra- tion of a mouse implanted with the device. Chemical solutions can flow into and out of the microchannel via an inlet silicone tube and an outlet silicone tube, respectively. (b) Cross-sectional schematic illustration of the im- plantable microfluidic device mounted on a mouse brain. Neural cells in the brain tissue can be observed by using 2PLSM.

Article Snippet: In vivo imaging using 2PLSM system The mouse implanted with the device was immobilized at an upright microscope 2PLSM system (BX61WI, FV1000, Olympus Co., Tokyo, Japan).

Techniques: Membrane

Journal: Journal of Photopolymer Science and Technology

Article Title: Implantable Microfluidic Device with Hydrogel Permeable Membrane for Delivering Chemical Compounds and Imaging Neural Cells in Living Mice

doi: 10.2494/photopolymer.29.513

Figure Lengend Snippet: Figure 5. A 2PLSM image of a dendrite of a neuron in the EGFP transgenic mouse implanted with the device. The structures of spine synapses were clearly observed as indicated by arrowheads.

Article Snippet: In vivo imaging using 2PLSM system The mouse implanted with the device was immobilized at an upright microscope 2PLSM system (BX61WI, FV1000, Olympus Co., Tokyo, Japan).

Techniques: Transgenic Assay

Journal: PLoS ONE

Article Title: The effects of tissue fixation on sequencing and transcript abundance of nucleic acids from microdissected liver samples of smallmouth bass ( Micropterus dolomieu )

doi: 10.1371/journal.pone.0236104

Figure Lengend Snippet: (A) Mean (+ standard error) of RNA and (B) DNA concentrations (μg/mm 3 ) of microdissected smallmouth bass liver samples fixed in 10% neutral buffered formalin (NBF), Z-Fix ® (ZF), and PAXgene® (PG) for 24 hours, 48 hours, 7 and 14 days. Samples preserved in 95% alcohol (ETOH) and RNALater® were included as controls.

Article Snippet: Tissues preserved in PG were removed from the PAXgene® Tissue FIX (Product # 765312, QIAGEN) after 4 hrs at RT, placed in the PAXgene® Tissue STABILIZER solution (Product # 765512, QIAGEN), and stored at 4°C for 24 hrs, 48 hrs, seven and 14 days.

Techniques:

Journal: PLoS ONE

Article Title: The effects of tissue fixation on sequencing and transcript abundance of nucleic acids from microdissected liver samples of smallmouth bass ( Micropterus dolomieu )

doi: 10.1371/journal.pone.0236104

Figure Lengend Snippet: (A) Mean RIN values of RNA and (B) fragment size (bp) of DNA from samples fixed in 10% neutral buffered formalin (NBF), Z-Fix (ZF) and PAXgene (PG) for 24 hours, 48 hours, 7 and 14 days. Samples preserved in 95% alcohol (ETOH) and RNAlater were included as controls.

Article Snippet: Tissues preserved in PG were removed from the PAXgene® Tissue FIX (Product # 765312, QIAGEN) after 4 hrs at RT, placed in the PAXgene® Tissue STABILIZER solution (Product # 765512, QIAGEN), and stored at 4°C for 24 hrs, 48 hrs, seven and 14 days.

Techniques: