Journal: Experimental eye research

Article Title: Pericyte loss via glutaredoxin2 downregulation aggravates diabetes-induced microvascular dysfunction.

doi: 10.1016/j.exer.2024.110025

Figure Lengend Snippet: Fig. 3. Grx2 knockdown inhibits pericyte function. Pericytes, both glucose-treated (30 mM for 48 h) and untreated, were transfected with either control shRNA or Grx2 mRNA-targeting shRNA. (A) WB was used to detect pericyte protein markers (PDGFR-β and NG2), apoptosis-related proteins (cleaved-caspase3/caspase3, Bax), and anti-apoptotic proteins (Bcl2). (B) The CCK-8 assay was performed to assess pericyte viability. (C) Flow cytometry analysis evaluated pericyte apoptosis. (D) Pericytes and endothelial cells were labeled green and red, respectively, to quantify pericyte recruitment to endothelial cells. (E) Pericyte-endothelial cell cocultures were performed to assess endothelial barrier function, by measuring the passage of 70-kDa FITC–dextran. Data are presented as mean ± SEM, n = 3. *P < 0.05; **P < 0.01; ***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Pericytes were exposed to different culture media for 48 h. Next, pericytes were seeded (1 × 104 cells/well) on the basolateral side of the filter, and equal number of endothelial cells were seeded on the apical side (JET, TCS018024, China), as described by Jiang et al. (2020) After 6 h co-culture in an incubator, 1 mg/mL FITC-Dextran fluorescent dye (MCE, HY-128868E) was added to the chamber, followed by 60 min incubation.

Techniques: Knockdown, Transfection, Control, shRNA, CCK-8 Assay, Flow Cytometry, Labeling

Journal: Experimental eye research

Article Title: Pericyte loss via glutaredoxin2 downregulation aggravates diabetes-induced microvascular dysfunction.

doi: 10.1016/j.exer.2024.110025

Figure Lengend Snippet: Fig. 4. Grx2 overexpression protects pericyte function. Pericytes, both glucose-treated (30 mM for 48 h) and untreated, were transfected with either control or Grx2 overexpression RNA. (A) WB was performed to detect pericyte protein markers (PDGFR-β and NG2), proteins associated with apoptosis (cleaved-caspase3/caspase3, Bax), and anti-apoptotic proteins (Bcl2). (B) The CCK-8 assay was performed to determine pericyte viability. (C) Flow cytometry was performed to assess apoptosis in pericytes. (D) Pericytes and endothelial cells were labeled green and red, respectively, to quantify pericyte recruitment to endothelial cells. (E) In coculture with endothelial cells, pericyte influence on endothelial barrier function was assessed by measuring the passage of 70-kDa FITC–dextran. Results are expressed as mean ± SEM, with n = 3. *P < 0.05; **P < 0.01; ***P < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Pericytes were exposed to different culture media for 48 h. Next, pericytes were seeded (1 × 104 cells/well) on the basolateral side of the filter, and equal number of endothelial cells were seeded on the apical side (JET, TCS018024, China), as described by Jiang et al. (2020) After 6 h co-culture in an incubator, 1 mg/mL FITC-Dextran fluorescent dye (MCE, HY-128868E) was added to the chamber, followed by 60 min incubation.

Techniques: Over Expression, Transfection, Control, CCK-8 Assay, Flow Cytometry, Labeling