Journal: Hepatology Communications

Article Title: Hedgehog Signaling Modulates Interleukin‐33‐Dependent Extrahepatic Bile Duct Cell Proliferation in Mice

doi: 10.1002/hep4.1295

Figure Lengend Snippet: Antibody List

Article Snippet: ST2 , Rabbit, 1:200; ProSci (3363) , Biotinylated donkey, ImmunoResearch (712‐065‐153).

Techniques:

Journal: Hepatology Communications

Article Title: Hedgehog Signaling Modulates Interleukin‐33‐Dependent Extrahepatic Bile Duct Cell Proliferation in Mice

doi: 10.1002/hep4.1295

Figure Lengend Snippet: Primer List

Article Snippet: ST2 , Rabbit, 1:200; ProSci (3363) , Biotinylated donkey, ImmunoResearch (712‐065‐153).

Techniques: Sequencing

Journal: Hepatology Communications

Article Title: Hedgehog Signaling Modulates Interleukin‐33‐Dependent Extrahepatic Bile Duct Cell Proliferation in Mice

doi: 10.1002/hep4.1295

Figure Lengend Snippet: HH signaling synergizes with IL‐33 in EHBD hyperplasia. (A) EHBDs from WT mice treated with either Veh or IL‐33 (1 µg/mouse/day for 4 days; necropsy on day 5) were analyzed by immunohistochemistry for ST2 expression (representative images of three samples). (B,C) EHBDs from WT, pCMV‐Shh , and Gli1 lacZ/lacZ mice treated with either Veh or mitogen IL‐33 intraperitoneally, as shown in (B) schema, were (C) examined macroscopically (representative photographs; ruler scale, 1 mm) and (D) histologically with H&E (scale bars, 20 µm; asterisks, BD lumen). (D) BD wall thickness from immunohistochemistry images was measured using ImageJ software and compared among WT, pCMV‐Shh, and Gli1 lacZ/lacZ mice treated with either Veh or IL‐33 (n = 7‐11 animals per group). Results are expressed as mean ± SEM; one‐way ANOVA; * P < 0.05, *** P < 0.001. Abbreviations: i.p., intraperitoneally; ns, not significant.

Article Snippet: ST2 , Rabbit, 1:200; ProSci (3363) , Biotinylated donkey, ImmunoResearch (712‐065‐153).

Techniques: Immunohistochemistry, Expressing, Software

Journal: Hepatology Communications

Article Title: Hedgehog Signaling Modulates Interleukin‐33‐Dependent Extrahepatic Bile Duct Cell Proliferation in Mice

doi: 10.1002/hep4.1295

Figure Lengend Snippet: IL‐33‐induced BDO cell proliferation in vitro . (A) Total RNA was isolated from the WT mouse‐derived organoids and analyzed for mRNA expression of St2 and Gli1 (n = 3 organoid lines). (B,C) BDOs were treated with Veh or recombinant IL‐33 (100 ng/mL) for 72 hours (day 3) starting 24 hours after passage of established BDOs and analyzed for growth (n = 6 technical replicates per group). (D,E) In a separate experiment, BDOs were treated with either Veh or pretreated for 1 hour with the NF‐κB inhibitor QNZ (1 µM) and then treated with recombinant IL‐33 (100 ng/mL) for 72 hours (day 3) after passaging of established BDOs. BDOs were analyzed for proliferation (n = 3 technical replicates per group). (D) The organoids were incubated with EdU for 9 hours to label proliferating cells (green) among all BDO cells (cell nuclei, DAPI [blue]; original magnification ×600). (E) ImageJ software was used to enumerate proliferating cells. Results are expressed as mean ± SEM; one‐way ANOVA (C,E); *** P < 0.001, **** P < 0.0001. Abbreviation: D0/D3, day 0/day 3.

Article Snippet: ST2 , Rabbit, 1:200; ProSci (3363) , Biotinylated donkey, ImmunoResearch (712‐065‐153).

Techniques: In Vitro, Isolation, Derivative Assay, Expressing, Recombinant, Passaging, Incubation, Software

Journal: Hepatology Communications

Article Title: Hedgehog Signaling Modulates Interleukin‐33‐Dependent Extrahepatic Bile Duct Cell Proliferation in Mice

doi: 10.1002/hep4.1295

Figure Lengend Snippet: Model of HH and IL‐33 synergy in IL‐33‐induced EHBD epithelial cell proliferation. IHH is secreted by epithelial cells and activates GLI1‐positive stromal cells. After EHBD injury, IL‐33 signals to GLI1‐positive stromal and epithelial cells through the ST2 receptor. IL‐33 signaling involves the transcriptional effector NF‐κB in epithelial cells. IL‐33 induces IL‐6 expression, which can signal to both epithelial and stromal cells. Increased epithelial cell proliferation induced by IL‐33 is partially HH dependent through a yet uncharacterized stromal factor.

Article Snippet: ST2 , Rabbit, 1:200; ProSci (3363) , Biotinylated donkey, ImmunoResearch (712‐065‐153).

Techniques: Expressing