Journal: International Journal of Molecular Sciences
Article Title: Recombinant Expression of a Novel Fungal Immunomodulatory Protein with Human Tumor Cell Antiproliferative Activity from Nectria haematococca
Figure Lengend Snippet: Purification and identification of rFIP-nha. ( A ) Tricine-SDS-PAGE analysis. GST-FIP-nha was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
Article Snippet: Gene Synthesis and Construction of the Expression Vector After optimizing codons according to the E. coli preference, FIP-nha (GenBank ID: EEU37941.1) was synthesized by GenScript (Nanjing, China).
Techniques: Purification, SDS Page, Molecular Weight, Sequencing, Mass Spectrometry