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  • 99
    ATCC sh sy5y derived fip in host cells
    Inducible expression of TBP/Q 36∼79 in <t>SH-SY5Y</t> cells. (A) Western blot analysis of induced TBP protein level relatively to endogenous TBP after 6 days induction (+Dox). (B) Western blot analysis of endogenous NFYA relatively to endogenous β-tubulin after 6 days induction (+Dox). (C) Isogenic SH-SY5Y cells were inducibly expressed TBP/Q 36∼79 for 6 days. Cells were fixed and stained with antibodies specific for TBP (red) and NFYA (yellow), and nuclei were counterstained with DAPI (blue). (The scale bar = 10 µm).
    Sh Sy5y Derived Fip In Host Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher fip in cho cells
    Inducible expression of TBP/Q 36∼79 in <t>SH-SY5Y</t> cells. (A) Western blot analysis of induced TBP protein level relatively to endogenous TBP after 6 days induction (+Dox). (B) Western blot analysis of endogenous NFYA relatively to endogenous β-tubulin after 6 days induction (+Dox). (C) Isogenic SH-SY5Y cells were inducibly expressed TBP/Q 36∼79 for 6 days. Cells were fixed and stained with antibodies specific for TBP (red) and NFYA (yellow), and nuclei were counterstained with DAPI (blue). (The scale bar = 10 µm).
    Fip In Cho Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    fips  (I-stem)
    85
    I-stem fips
    Inducible expression of TBP/Q 36∼79 in <t>SH-SY5Y</t> cells. (A) Western blot analysis of induced TBP protein level relatively to endogenous TBP after 6 days induction (+Dox). (B) Western blot analysis of endogenous NFYA relatively to endogenous β-tubulin after 6 days induction (+Dox). (C) Isogenic SH-SY5Y cells were inducibly expressed TBP/Q 36∼79 for 6 days. Cells were fixed and stained with antibodies specific for TBP (red) and NFYA (yellow), and nuclei were counterstained with DAPI (blue). (The scale bar = 10 µm).
    Fips, supplied by I-stem, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pfizer Inc fip vaccine
    Inducible expression of TBP/Q 36∼79 in <t>SH-SY5Y</t> cells. (A) Western blot analysis of induced TBP protein level relatively to endogenous TBP after 6 days induction (+Dox). (B) Western blot analysis of endogenous NFYA relatively to endogenous β-tubulin after 6 days induction (+Dox). (C) Isogenic SH-SY5Y cells were inducibly expressed TBP/Q 36∼79 for 6 days. Cells were fixed and stained with antibodies specific for TBP (red) and NFYA (yellow), and nuclei were counterstained with DAPI (blue). (The scale bar = 10 µm).
    Fip Vaccine, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    GenScript fip nha
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fip Nha, supplied by GenScript, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Pfizer Inc primucell fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Primucell Fip, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    BASF insecticide fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Insecticide Fip, supplied by BASF, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Pfizer Animal Health primucell fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Primucell Fip, supplied by Pfizer Animal Health, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Projects Activity fip microscope
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fip Microscope, supplied by Projects Activity, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Zoetis vaccine against fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Vaccine Against Fip, supplied by Zoetis, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore fip specific shrnas
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fip Specific Shrnas, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Antech Diagnostics fip virus
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fip Virus, supplied by Antech Diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    IDEXX fip antibody test kit diasystems celisa fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fip Antibody Test Kit Diasystems Celisa Fip, supplied by IDEXX, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bachem fusion inhibiting peptide fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fusion Inhibiting Peptide Fip, supplied by Bachem, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bachem fusion inhibitory peptide fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fusion Inhibitory Peptide Fip, supplied by Bachem, used in various techniques. Bioz Stars score: 85/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    IDEXX fip virus realpcr test
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fip Virus Realpcr Test, supplied by IDEXX, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bachem mv specific fusion inhibitor fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Mv Specific Fusion Inhibitor Fip, supplied by Bachem, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Avantor 700 fip u g pepsin
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    700 Fip U G Pepsin, supplied by Avantor, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Mine Safety Appliances five digit fips codes
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Five Digit Fips Codes, supplied by Mine Safety Appliances, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher cell culture fip in t rex hela
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Cell Culture Fip In T Rex Hela, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Pfizer Inc vaccines against fip coronavirus fipv
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Vaccines Against Fip Coronavirus Fipv, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mine Safety Appliances county federal information processing standards fips codes
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
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    84
    Pfizer Animal Health administration felocell fip in pfizer animal health mlv
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Administration Felocell Fip In Pfizer Animal Health Mlv, supplied by Pfizer Animal Health, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Bachem fusion inhibiting peptide z d phe l phe gly oh fip
    Purification and identification of <t>rFIP-nha.</t> ( A ) Tricine-SDS-PAGE analysis. <t>GST-FIP-nha</t> was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.
    Fusion Inhibiting Peptide Z D Phe L Phe Gly Oh Fip, supplied by Bachem, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inducible expression of TBP/Q 36∼79 in SH-SY5Y cells. (A) Western blot analysis of induced TBP protein level relatively to endogenous TBP after 6 days induction (+Dox). (B) Western blot analysis of endogenous NFYA relatively to endogenous β-tubulin after 6 days induction (+Dox). (C) Isogenic SH-SY5Y cells were inducibly expressed TBP/Q 36∼79 for 6 days. Cells were fixed and stained with antibodies specific for TBP (red) and NFYA (yellow), and nuclei were counterstained with DAPI (blue). (The scale bar = 10 µm).

    Journal: PLoS ONE

    Article Title: Role of the CCAAT-Binding Protein NFY in SCA17 Pathogenesis

    doi: 10.1371/journal.pone.0035302

    Figure Lengend Snippet: Inducible expression of TBP/Q 36∼79 in SH-SY5Y cells. (A) Western blot analysis of induced TBP protein level relatively to endogenous TBP after 6 days induction (+Dox). (B) Western blot analysis of endogenous NFYA relatively to endogenous β-tubulin after 6 days induction (+Dox). (C) Isogenic SH-SY5Y cells were inducibly expressed TBP/Q 36∼79 for 6 days. Cells were fixed and stained with antibodies specific for TBP (red) and NFYA (yellow), and nuclei were counterstained with DAPI (blue). (The scale bar = 10 µm).

    Article Snippet: Firstly SH-SY5Y-derived FIp-In host cells were generated from independent integration of plasmids pcDNA6/TR (a plasmid expressing the Tet repressor; selected with 5 µg/ml blasticidin) and pFRT/lacZeo (a plasmid containing the Flp Recombination Target (FRT) site; selected with 100 µg/ml Zocin) into the genome of SH-SY5Y cells (ATCC No. CRL-2266).

    Techniques: Expressing, Western Blot, Staining

    Purification and identification of rFIP-nha. ( A ) Tricine-SDS-PAGE analysis. GST-FIP-nha was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.

    Journal: International Journal of Molecular Sciences

    Article Title: Recombinant Expression of a Novel Fungal Immunomodulatory Protein with Human Tumor Cell Antiproliferative Activity from Nectria haematococca

    doi: 10.3390/ijms151017751

    Figure Lengend Snippet: Purification and identification of rFIP-nha. ( A ) Tricine-SDS-PAGE analysis. GST-FIP-nha was purified using a GST column, digested with thrombin and eluted with PBS and glutathione. Lane 1: Eluate with PBS. Lanes 2 and 3: Eluates with glutathione. Lane 4: Standard protein molecular weight markers. Arrows 1, 2, and 3 indicated the purified rFIP-nha, GST, and GST-FIP-nha, respectively; ( B ) Amino acid sequence of deduced FIP-nha. Nine peptide fragments consisting of 91 residues identified by MALDI-MS are underlined.

    Article Snippet: Gene Synthesis and Construction of the Expression Vector After optimizing codons according to the E. coli preference, FIP-nha (GenBank ID: EEU37941.1) was synthesized by GenScript (Nanjing, China).

    Techniques: Purification, SDS Page, Molecular Weight, Sequencing, Mass Spectrometry