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Journal: Current biology : CB

Article Title: A paradoxical kind of sleep in Drosophila melanogaster

doi: 10.1016/j.cub.2020.10.081

Figure Lengend Snippet: Key Resources Table:

Article Snippet: [ 66 ] Bloomington ID 49032 UAS-Chrimson (P[20xUAS-IVS-CsChrimson.mVenus]attp18) Vivek Jayaraman, JFRC [ 36 ] Bloomington ID 55134 lexAop-Syn21-nlsGCaMP6f-p10 in VIE-260b This paper (provided by Barry J. Dickson) N/A 10xUAS-Chrimson88-tdT(attP18) [ 67 ] N/A Nsyb-LexAp65(attP2) [ 68 ] N/A 13XLexAop2-IVS-Syn21-mPA-p10 (VK00005) [ 68 ] N/A Software and Algorithms Drosophila ARousal Tracking (DART) system BFK-LAB [ 34 ] http://www.bfklab.com/dart MatLab analysis scripts This paper https://espace.library.uq.edu.au/ CeTran (3.4) software Buritracker http://buridan.sourceforge.net/ MatLab v2018b Mathworks https://au.mathworks.com/products/get-matlab.html Prism 8.1.2 Graphpad https://www.graphpad.com/scientific-software/prism/ ImageJ NIH https://imagej.net FiJi FijiSc [ 70 ] https://imagej.net/Fiji ThorLabs Imaging software v3.0 Thorlabs https://www.thorlabs.com/navigation.cfm?Guide_ID=2191 AxoGraph 1.4.4 Axon Instruments https://axograph.com/index.php?option=com_content&view=article&id=12&Itemid=136 Other 65 mm glass tubes Trikinetics PGT7x65 617 nm Luxeon Rebel LED Luxeon Star LEDs, Ontario, Canada MR-H2060-10S Sleep Nullifying Apparatus (SNAP) [ 37 ] N/A LED arena for visual attention experiments [ 37 ] N/A ThorLabs Bergamo series 2 multiphoton microscope ThorLabs BERGAMO Ti:Sapphire laser (920nm tuned) Spectra Physics Mai Tai Deepsee High Speed Pockels Cell Module Thorlabs BCM-PCA100 Galvo-resonant scanner Thorlabs OPX1100 Nikon CFI APO 40XW NIR objective Thorlabs N40X-NIR Piezo Focus Motor Thorlabs PI-P725 High Sensitivity GaAsP PMT ThorLabs PMT2000 594 dichroic beam splitter ThorLabs 22-0104 680 nm Shortpass Dichroic Thorlabs 22-0312 525/25nm band pass filter ThorLabs 22-0045 617nm LED ThorLabs M617F1 405nm LED ThorLabs M405L4 4 channel LED driver Thorlabs DC4104 Firefly MV 0.3MP camera FLIR Systems FMVU-03MTM-CS Custom air puff generator This paper N/A 1400 A Digitizer Axon Digidata N/A Custom sine wave generator This paper N/A Open in a separate window Key Resources Table: Calcium imaging reveals distinct spontaneous sleep stages in the fly brain Short bouts of quiescence in Drosophila may involve an active sleep stage Optogenetic activation of a sleep switch promotes wake-like brain activity Prolonged activation of the sleep switch corrects visual attention defects

Techniques: Recombinant, Software, Imaging, Microscopy