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    Fibronectin Mouse Monoclonal
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    Thermo Fisher fibronectin
    Fibronectin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin - by Bioz Stars, 2021-04
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    99
    Millipore fibronectin
    Fibronectin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin - by Bioz Stars, 2021-04
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    86
    Becton Dickinson fibronectin
    Chronic and acute exposure to <t>fibronectin</t> increases tyrosine phosphorylation of src tyrosine kinase and TRPV1 in trigeminal ganglia cells. A . Immunoprecipitation of primary cultured trigeminal ganglia grown on fibronectin (FN) or poly-D-lysine (PDL) with antibodies directed against phospho-tyrosine residues (P-Tyr), and blotted for TRPV1 and src. B . Immunoprecipitation of primary cultured trigeminal ganglia treated with FN (10 μg/ml, 20 min) following pre-treatment with PP2 (src tyrosine kinase inhibitor, 1mM, 5 min) with antibodies directed against phospho-tyrosine residues (P-Tyr), and blotted for TRPV1 and src expression.
    Fibronectin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin - by Bioz Stars, 2021-04
    86/100 stars
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    99
    Abcam fibronectin
    SOX2 knockdown inhibited the EMT process and the growth of BCC cells. Cell proliferation was (A) inhibited by SOX2 knockdown and (B) promoted by SOX2 overexpression. (C) SOX2 knockdown upregulated the expression of epithelial marker E-cadherin, and decreased the levels of mesenchymal markers Vimentin and <t>Fibronectin</t> in BCC cells. (D) SOX2 overexpression downregulated the expression of epithelial marker E-cadherin, and increased the levels of mesenchymal markers Vimentin and Fibronectin in BCC cells. The effect of (E) siRNA-SOX2 and (F) pSOX2 transfection on the expression levels of EMT markers in BCC cells was determined by immunofluorescence assay. Magnification for all images, ×40. **P
    Fibronectin, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fibronectin/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fibronectin - by Bioz Stars, 2021-04
    99/100 stars
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    Highly purified Fibronectin protein
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    Category Protein Products Peptides Fibronectin Analog Size 5 mg
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    Chronic and acute exposure to fibronectin increases tyrosine phosphorylation of src tyrosine kinase and TRPV1 in trigeminal ganglia cells. A . Immunoprecipitation of primary cultured trigeminal ganglia grown on fibronectin (FN) or poly-D-lysine (PDL) with antibodies directed against phospho-tyrosine residues (P-Tyr), and blotted for TRPV1 and src. B . Immunoprecipitation of primary cultured trigeminal ganglia treated with FN (10 μg/ml, 20 min) following pre-treatment with PP2 (src tyrosine kinase inhibitor, 1mM, 5 min) with antibodies directed against phospho-tyrosine residues (P-Tyr), and blotted for TRPV1 and src expression.

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Chronic and acute exposure to fibronectin increases tyrosine phosphorylation of src tyrosine kinase and TRPV1 in trigeminal ganglia cells. A . Immunoprecipitation of primary cultured trigeminal ganglia grown on fibronectin (FN) or poly-D-lysine (PDL) with antibodies directed against phospho-tyrosine residues (P-Tyr), and blotted for TRPV1 and src. B . Immunoprecipitation of primary cultured trigeminal ganglia treated with FN (10 μg/ml, 20 min) following pre-treatment with PP2 (src tyrosine kinase inhibitor, 1mM, 5 min) with antibodies directed against phospho-tyrosine residues (P-Tyr), and blotted for TRPV1 and src expression.

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Immunoprecipitation, Cell Culture, Expressing

    Acute exposure to fibronectin increases CAP-responsive neuropeptide release in trigeminal ganglia cells in a PP2-sensitive manner. Primary cultures of trigeminal ganglia were prr-treated with vehicle, fibronectin (FN, 10 μg/ml, 20 min), or PP2 (1 μM, 5 min) and FN (10 μg/ml, 20 min), and then treated with increasing concentrations of capsaicin (CAP) to stimulate calcitonin gene-related peptide (CGRP) release, as determined by RIA.

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Acute exposure to fibronectin increases CAP-responsive neuropeptide release in trigeminal ganglia cells in a PP2-sensitive manner. Primary cultures of trigeminal ganglia were prr-treated with vehicle, fibronectin (FN, 10 μg/ml, 20 min), or PP2 (1 μM, 5 min) and FN (10 μg/ml, 20 min), and then treated with increasing concentrations of capsaicin (CAP) to stimulate calcitonin gene-related peptide (CGRP) release, as determined by RIA.

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques:

    Acute exposure to fibronectin increases plasma membrane surface expression of TRPV1 in trigeminal ganglia cells. A . Primary cultures of trigeminal ganglia were treated with vehicle or fibronectin (FN, 10 μg/ml, 20 min), and biotinylated to identify surface proteins. Streptavidin-agarose precipitates (Strept) and 50 μg total cell lysate (CL) aliquots were probed for TRPV1, β1 integrin, and α5 integrin expression. Densitometry measurements of streptavidin-precipitated TRPV1 normalized to those for streptavidin-precipitated β1 integrin ( B ), streptavidin-precipitated α5 integrin ( C ), and CL TRPV1 ( D ). E. Primary cultures of trigeminal ganglia were pre-treated with the src tyrosine kinase inhibitor PP2 (1 μM, 5 min), and then with FN (10 μg/ml, 20 min), and biotinylated to identify surface proteins. Streptavidin-agarose precipitates (Strept) and 50 μg total cell lysate (CL) aliquots were probed for TRPV1, and β1 integrin. *p

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Acute exposure to fibronectin increases plasma membrane surface expression of TRPV1 in trigeminal ganglia cells. A . Primary cultures of trigeminal ganglia were treated with vehicle or fibronectin (FN, 10 μg/ml, 20 min), and biotinylated to identify surface proteins. Streptavidin-agarose precipitates (Strept) and 50 μg total cell lysate (CL) aliquots were probed for TRPV1, β1 integrin, and α5 integrin expression. Densitometry measurements of streptavidin-precipitated TRPV1 normalized to those for streptavidin-precipitated β1 integrin ( B ), streptavidin-precipitated α5 integrin ( C ), and CL TRPV1 ( D ). E. Primary cultures of trigeminal ganglia were pre-treated with the src tyrosine kinase inhibitor PP2 (1 μM, 5 min), and then with FN (10 μg/ml, 20 min), and biotinylated to identify surface proteins. Streptavidin-agarose precipitates (Strept) and 50 μg total cell lysate (CL) aliquots were probed for TRPV1, and β1 integrin. *p

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Expressing

    Fibronectin integrin receptor subunits co-express with TRPV1 in intact trigeminal ganglia. Fixed slices of intact trigeminal ganglia probed for TRPV1 expression (red) with integrin subunits known to heterodimerize and recognize fibronectin (green): A . β1, B . β3, C . β5, D . β6, E . β7, F . β8, G . α4, H . α5, I . αv, and J . α11b. Yellow bars indicate 100 μm.

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Fibronectin integrin receptor subunits co-express with TRPV1 in intact trigeminal ganglia. Fixed slices of intact trigeminal ganglia probed for TRPV1 expression (red) with integrin subunits known to heterodimerize and recognize fibronectin (green): A . β1, B . β3, C . β5, D . β6, E . β7, F . β8, G . α4, H . α5, I . αv, and J . α11b. Yellow bars indicate 100 μm.

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Expressing

    Chronic exposure to fibronectin increases capsaicin-mediated neuropeptide release from trigeminal ganglia. A . Primary cultures of trigeminal ganglia were grown on poly-D-lysine (PDL), laminin (Lam), collagen type IV (Coll IV) or fibronectin (FN), and treated with increasing concentrations of capsaicin (CAP) to stimulate calcitonin gene-related peptide (CGRP) release, as determined by RIA. B. Primary cultures of trigeminal ganglia grown on PDL, Lam, Coll IV or FN subjected to 0.01N HCL- or 50 mM KCl-stimulated CGRP release. C. Primary cultures of trigeminal ganglia grown on PDL, Lam, Coll IV or FN revealed similar staining patterns of CGRP in TRPV1-positve cells (CGRP/TRPV1), and of TRPV1 in CGRP-positive cells (TRPV1/CGRP). *p

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Chronic exposure to fibronectin increases capsaicin-mediated neuropeptide release from trigeminal ganglia. A . Primary cultures of trigeminal ganglia were grown on poly-D-lysine (PDL), laminin (Lam), collagen type IV (Coll IV) or fibronectin (FN), and treated with increasing concentrations of capsaicin (CAP) to stimulate calcitonin gene-related peptide (CGRP) release, as determined by RIA. B. Primary cultures of trigeminal ganglia grown on PDL, Lam, Coll IV or FN subjected to 0.01N HCL- or 50 mM KCl-stimulated CGRP release. C. Primary cultures of trigeminal ganglia grown on PDL, Lam, Coll IV or FN revealed similar staining patterns of CGRP in TRPV1-positve cells (CGRP/TRPV1), and of TRPV1 in CGRP-positive cells (TRPV1/CGRP). *p

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Laser Capture Microdissection, Staining

    Chronic exposure to fibronectin increases the number of capsaicin-sensitive trigeminal ganglia cells. A . Quantification of total calcium accumulation in primary cultured trigeminal ganglia cells grown on PDL (light grey) or FN (dark grey) in response to 50 nM CAP. B . Percentage of CAP (50nM)-responsive neurons observed of total pre-determined population of primary cultured trigeminal ganglia, grown on either PDL or FN (actual number shown in parentheses). C . Percentage of TRPV1-positive neurons of total NeuN-positive neurons from primary cultured trigeminal ganglia grown on PDL or FN. *p

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Chronic exposure to fibronectin increases the number of capsaicin-sensitive trigeminal ganglia cells. A . Quantification of total calcium accumulation in primary cultured trigeminal ganglia cells grown on PDL (light grey) or FN (dark grey) in response to 50 nM CAP. B . Percentage of CAP (50nM)-responsive neurons observed of total pre-determined population of primary cultured trigeminal ganglia, grown on either PDL or FN (actual number shown in parentheses). C . Percentage of TRPV1-positive neurons of total NeuN-positive neurons from primary cultured trigeminal ganglia grown on PDL or FN. *p

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Cell Culture

    Chronic exposure to fibronectin increases plasma membrane surface expression of TRPV1 in trigeminal ganglia cells. A . Primary cultures of trigeminal ganglia were grown on poly-D-lysine (PDL) or fibronectin (FN), and biotinylated to identify surface proteins. Streptavidin-agarose precipitates (Strept) and 50 μg total cell lysate (CL) aliquots were probed for TRPV1, β1 integrin, α5 integrin, and β-actin expression. Densitometry measurements of streptavidin-precipitated TRPV1 normalized to those for streptavidin-precipitated β1 integrin ( B ), streptavidin-precipitated α5 integrin ( C ), and CL TRPV1 ( D ). *p

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Chronic exposure to fibronectin increases plasma membrane surface expression of TRPV1 in trigeminal ganglia cells. A . Primary cultures of trigeminal ganglia were grown on poly-D-lysine (PDL) or fibronectin (FN), and biotinylated to identify surface proteins. Streptavidin-agarose precipitates (Strept) and 50 μg total cell lysate (CL) aliquots were probed for TRPV1, β1 integrin, α5 integrin, and β-actin expression. Densitometry measurements of streptavidin-precipitated TRPV1 normalized to those for streptavidin-precipitated β1 integrin ( B ), streptavidin-precipitated α5 integrin ( C ), and CL TRPV1 ( D ). *p

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Expressing

    Fibronectin integrin receptor subunits co-express with TRPV1 in cultured trigeminal ganglia. Primary cultures of trigeminal ganglia probed for TRPV1 expression (red) with integrin subunits known to heterodimerize and recognize fibronectin (green): A . β1, B . β3, C . β5, D . β6, E . β7, F . β8, G . α4, H . α5, I . α11b, and J . αv. Yellow bars indicate 100 μm. K. 30 randomly chosen coverslips from each immunocytochemical pairing were counted for total TRPV1-positive cells, total integrin subunit-positive cells, and cells that co-express both TRPV1 and the targeted integrin subunit. Results are displayed as number of co-expressing cells per total of each singular protein, dark grey bars indicate percentage of integrin-subunit-positive cells that co-express TRPV1, light grey bars indicate percentage of TRPV1-positve cells that co-express the integrin subunit.

    Journal: Journal of neurochemistry

    Article Title: Fibronectin Stimulates TRPV1 Translocation in Primary Sensory Neurons

    doi: 10.1111/j.1471-4159.2008.05779.x

    Figure Lengend Snippet: Fibronectin integrin receptor subunits co-express with TRPV1 in cultured trigeminal ganglia. Primary cultures of trigeminal ganglia probed for TRPV1 expression (red) with integrin subunits known to heterodimerize and recognize fibronectin (green): A . β1, B . β3, C . β5, D . β6, E . β7, F . β8, G . α4, H . α5, I . α11b, and J . αv. Yellow bars indicate 100 μm. K. 30 randomly chosen coverslips from each immunocytochemical pairing were counted for total TRPV1-positive cells, total integrin subunit-positive cells, and cells that co-express both TRPV1 and the targeted integrin subunit. Results are displayed as number of co-expressing cells per total of each singular protein, dark grey bars indicate percentage of integrin-subunit-positive cells that co-express TRPV1, light grey bars indicate percentage of TRPV1-positve cells that co-express the integrin subunit.

    Article Snippet: TGG were plated onto 24-well plates coated with poly-D lysine, laminin, collagen type IV or fibronectin (BD Biosciences, Franklin Lakes, NJ).

    Techniques: Cell Culture, Expressing

    SOX2 knockdown inhibited the EMT process and the growth of BCC cells. Cell proliferation was (A) inhibited by SOX2 knockdown and (B) promoted by SOX2 overexpression. (C) SOX2 knockdown upregulated the expression of epithelial marker E-cadherin, and decreased the levels of mesenchymal markers Vimentin and Fibronectin in BCC cells. (D) SOX2 overexpression downregulated the expression of epithelial marker E-cadherin, and increased the levels of mesenchymal markers Vimentin and Fibronectin in BCC cells. The effect of (E) siRNA-SOX2 and (F) pSOX2 transfection on the expression levels of EMT markers in BCC cells was determined by immunofluorescence assay. Magnification for all images, ×40. **P

    Journal: Oncology Letters

    Article Title: SOX2 knockdown inhibits the migration and invasion of basal cell carcinoma cells by targeting the SRPK1-mediated PI3K/AKT signaling pathway

    doi: 10.3892/ol.2018.9810

    Figure Lengend Snippet: SOX2 knockdown inhibited the EMT process and the growth of BCC cells. Cell proliferation was (A) inhibited by SOX2 knockdown and (B) promoted by SOX2 overexpression. (C) SOX2 knockdown upregulated the expression of epithelial marker E-cadherin, and decreased the levels of mesenchymal markers Vimentin and Fibronectin in BCC cells. (D) SOX2 overexpression downregulated the expression of epithelial marker E-cadherin, and increased the levels of mesenchymal markers Vimentin and Fibronectin in BCC cells. The effect of (E) siRNA-SOX2 and (F) pSOX2 transfection on the expression levels of EMT markers in BCC cells was determined by immunofluorescence assay. Magnification for all images, ×40. **P

    Article Snippet: Subsequently, the cells were incubated for 2 h at 37°C with the indicated antibodies as follows: E-cadherin (1:1,000; ab40772), Vimentin (1:1,000; ab92547), Fibronectin (1:1,000; ab2413; all purchased from Abcam).

    Techniques: Over Expression, Expressing, Marker, Transfection, Immunofluorescence