Journal: Polymers

Article Title: Innovative Electrospun Nanofiber Mats Based on Polylactic Acid Composited with Silver Nanoparticles for Medical Applications

doi: 10.3390/polym16030409

Figure Lengend Snippet: Percentage of cell viability of L929 mouse fibroblast cells by MTT assay of different mats over 24 h of culture. NC: Cells and culture media without any samples. The experiments were repeated in triplicate.

Article Snippet: L-929 cells, a mouse fibroblast cell line (Elabscience, Houston, TX, USA), were cultured in 96-well plates at a density of 7.5 × 10 4 cells/mL in a completed MEM medium containing 10% FBS and 1% penicillin–streptomycin ( v / v ) and incubated for 24 h in a humidified atmosphere of 5% CO 2 in an incubator at 37 °C.

Techniques: MTT Assay

Journal: ACS Nano

Article Title: Multifunctional Injectable Bioadhesive with Toll-like Receptor 4 and Myeloid Differentiation Factor 2 Antagonistic Anti-inflammatory Potential for Periodontal Regeneration

doi: 10.1021/acsnano.4c15922

Figure Lengend Snippet: Biocompatibility of the iCPC@MgO composite hydrogel and its effects on hPDLSCs osteogenic differentiation. (A) CCK8 assay and (B) live/dead staining for hPDLSCs’ viability after culturing on the 4 mg mL –1 hydrogel in each well. (C) Alkaline phosphatase activity assay was used to evaluate the early osteogenic abilities after culturing on 4 mg mL –1 hydrogel at 4 and 7 days. (D) ARS at 21 days of culturing on the 4 mg mL –1 hydrogel. (E) Semiquantification of calcium nodules ( n = 6 biologically independent replicates). (F,G) Western blot assay to detect the protein levels of the Wnt/β-catenin pathway at 21 days of culturing on the hydrogel. (H) Runx2, β catenin, and GSK 3β relative mRNA expression in hPDLSCs at 21 days of culturing on the hydrogel. Data are mean ± s.d. Statistical significance was analyzed by one-way ANOVA (* p < 0.05, ** p < 0.01, *** p < 0.001, ns represents nonsignificance).

Article Snippet: Human periodontal ligament cells (hPDLSCs) and human gingival fibroblasts (HGFs) were commercially obtained from the Elabscience Biotechnology Co. Ltd., Wuhan, China.

Techniques: CCK-8 Assay, Staining, ALP Activity Assay, Western Blot, Expressing