Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway

doi: 10.1111/jcmm.17934

Figure Lengend Snippet: FGF5 overexpression prevents SCI in mice. (A) Mice were exposed to Ctrl or SCI surgery, and FGF5 mRNA level in the spinal cord was detected at indicating times. (B) FGF5 level was detected using an ELISA kit. (C, D) Mice were intraspinally injected with 2 μL lentivirus carrying FGF5 to overexpress FGF5 in the spinal cord, and FGF5 mRNA and protein levels were detected 2 weeks post‐injection. (E) Mice were overexpressed with FGF5 using lentiviral vectors and then were exposed to SCI or sham surgery 2 weeks post‐injection. BMS score, from 0 (no ankle movement) to 9 (normal gait), was determined at indicating times. (F, G) Sensitivities to mechanical and thermal stimulation were determined 28 days after SCI. (H) Extravasation of Evans blue dye was determined 28 days after SCI. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. * p < 0.05.

Article Snippet: FGF5 in mouse spinal cord and human serum samples were detected using Mouse FGF5 ELISA Kit (#CSB‐EL008632MO; CUSABIO) or Human FGF5 ELISA Kit (#CSB‐EL008632HU; CUSABIO), respectively according to the manufacturer's instructions.

Techniques: Over Expression, Enzyme-linked Immunosorbent Assay, Injection, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway

doi: 10.1111/jcmm.17934

Figure Lengend Snippet: FGF5 knockdown exacerbates SCI in mice. (A, B) Mice were intraspinally injected with 2 μL lentivirus carrying shFGF5 to knockdown FGF5 in the spinal cord, and FGF5 mRNA and protein levels were detected 2 weeks post‐injection. (C) Mice were injected with shFGF5, and then were exposed to SCI or sham surgery 2 weeks post‐injection. BMS score, from 0 (no ankle movement) to 9 (normal gait), was determined at indicating times. (D, E) Sensitivities to mechanical and thermal stimulation were determined 28 days after SCI. (F) Extravasation of Evans blue dye was determined 28 days after SCI. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. * p < 0.05.

Article Snippet: FGF5 in mouse spinal cord and human serum samples were detected using Mouse FGF5 ELISA Kit (#CSB‐EL008632MO; CUSABIO) or Human FGF5 ELISA Kit (#CSB‐EL008632HU; CUSABIO), respectively according to the manufacturer's instructions.

Techniques: Knockdown, Injection, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway

doi: 10.1111/jcmm.17934

Figure Lengend Snippet: FGF5 overexpression reduces inflammation and oxidative stress in SCI mice. (A) Mice were overexpressed with FGF5 using lentiviral vectors, and then were exposed to SCI or sham surgery 2 weeks post‐injection. IL‐6 and TNF‐α levels in the spinal cord were detected. (B) MPO activity in the spinal cord. (C) Western blot images and quantification of p65 phosphorylation. (D) Relative NF‐κB transcription activity. (E, F) Relative levels of NRF2 protein and transcription activity. (G) ROS level detected by DCFH‐DA probe. (H) Quantification of hydrogen peroxide and superoxide anion in spinal cord. (I) The levels of MDA, 3‐NT and 8‐OHdG. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. * p < 0.05.

Article Snippet: FGF5 in mouse spinal cord and human serum samples were detected using Mouse FGF5 ELISA Kit (#CSB‐EL008632MO; CUSABIO) or Human FGF5 ELISA Kit (#CSB‐EL008632HU; CUSABIO), respectively according to the manufacturer's instructions.

Techniques: Over Expression, Injection, Activity Assay, Western Blot, Phospho-proteomics, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway

doi: 10.1111/jcmm.17934

Figure Lengend Snippet: FGF5 knockdown elevates inflammation and oxidative stress in SCI mice. (A) Mice were injected with shFGF5, and then were exposed to SCI or sham surgery 2 weeks post‐injection. IL‐6 and TNF‐α levels in the spinal cord were detected. (B) MPO activity in the spinal cord. (C) Western blot images and quantification of p65 phosphorylation. (D) Relative NF‐κB transcription activity. (E, F) Relative levels of NRF2 protein and transcription activity. (G) ROS level detected by DCFH‐DA probe. (H) Quantification of hydrogen peroxide and superoxide anion in spinal cord. (I) The levels of MDA, 3‐NT and 8‐OHdG. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. * p < 0.05.

Article Snippet: FGF5 in mouse spinal cord and human serum samples were detected using Mouse FGF5 ELISA Kit (#CSB‐EL008632MO; CUSABIO) or Human FGF5 ELISA Kit (#CSB‐EL008632HU; CUSABIO), respectively according to the manufacturer's instructions.

Techniques: Knockdown, Injection, Activity Assay, Western Blot, Phospho-proteomics, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway

doi: 10.1111/jcmm.17934

Figure Lengend Snippet: FGF5 overexpression reduces inflammation, oxidative stress and SCI through activating AMPK. (A) Mice were overexpressed with FGF5 using lentiviral vectors, and then were exposed to SCI or sham surgery 2 weeks post‐injection. Western blot images and quantification of AMPK phosphorylation in the spinal cord were detected. (B) To inhibit AMPK, mice with or without FGF5 overexpression were intraperitoneally injected with CC every 2 days 1 week pre‐SCI. IL‐6 and TNF‐α levels in the spinal cord were detected. (C) ROS level detected by DCFH‐DA probe. (D) The levels of MDA, 3‐NT and 8‐OHdG. (E) BMS score, from 0 (no ankle movement) to 9 (normal gait), was determined at indicating times. (F, G) Sensitivities to mechanical and thermal stimulation were determined 28 days after SCI. (H) Extravasation of Evans blue dye was determined 28 days after SCI. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. * p < 0.05.

Article Snippet: FGF5 in mouse spinal cord and human serum samples were detected using Mouse FGF5 ELISA Kit (#CSB‐EL008632MO; CUSABIO) or Human FGF5 ELISA Kit (#CSB‐EL008632HU; CUSABIO), respectively according to the manufacturer's instructions.

Techniques: Over Expression, Injection, Western Blot, Phospho-proteomics, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway

doi: 10.1111/jcmm.17934

Figure Lengend Snippet: FGF5 overexpression activates AMPK through cAMP/PKA pathway. (A, B) Mice were intraspinally injected with 2 μL lentivirus carrying FGF5 to overexpress FGF5 in the spinal cord, and cAMP level and PKA activity were detected 2 weeks post‐injection. (C) To inhibit PKA, mice with or without FGF5 overexpression were intraperitoneally injected with H89 every 2 days 1 week pre‐SCI. Western blot images and quantification of AMPK phosphorylation in the spinal cord were detected. (D) ROS level detected by DCFH‐DA probe. (E) The levels of MDA, 3‐NT and 8‐OHdG. (F) IL‐6 and TNF‐α levels in the spinal cord were detected. (G) BMS score, from 0 (no ankle movement) to 9 (normal gait), was determined at indicating times. (H, I) Sensitivities to mechanical and thermal stimulation were determined 28 days after SCI. (J) Extravasation of Evans blue dye was determined 28 days after SCI. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. * p < 0.05.

Article Snippet: FGF5 in mouse spinal cord and human serum samples were detected using Mouse FGF5 ELISA Kit (#CSB‐EL008632MO; CUSABIO) or Human FGF5 ELISA Kit (#CSB‐EL008632HU; CUSABIO), respectively according to the manufacturer's instructions.

Techniques: Over Expression, Injection, Activity Assay, Western Blot, Phospho-proteomics, Standard Deviation

Journal: Journal of Cellular and Molecular Medicine

Article Title: Fibroblast growth factor 5 protects against spinal cord injury through activating AMPK pathway

doi: 10.1111/jcmm.17934

Figure Lengend Snippet: Serum FGF5 level positively correlates with the sensory and motor function in SCI patients. (A) Serum levels of FGF5 in Ctrl and SCI patients. (B, C) SCI patients were divided into four groups according to serum FGF5 levels, and ASIA sensation and motor scores were measured at indicating groups. n = 56 for Ctrl group and n = 92 for SCI group. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. * p < 0.05.

Article Snippet: FGF5 in mouse spinal cord and human serum samples were detected using Mouse FGF5 ELISA Kit (#CSB‐EL008632MO; CUSABIO) or Human FGF5 ELISA Kit (#CSB‐EL008632HU; CUSABIO), respectively according to the manufacturer's instructions.

Techniques: Standard Deviation

Journal: Biochemical and biophysical research communications

Article Title: FGF5 alleviated acute lung injury via AKT signal pathway in endothelial cells.

doi: 10.1016/j.bbrc.2022.09.112

Figure Lengend Snippet: Fig. 1. FGF5 was downregulated in endothelial cells under the stimulation of LPS. A: Double immunofluorescence staining of lungs for CD31 and FGF5 (Representative endothelial cells were indicated by white arrows). B: Immunohistochemical staining for FGF5 in the lungs. C: The relative mRNA level of FGF5 in HUVECs was shown as a bar chart (n ¼ 4). DeE: The representative bands and the bar graph were shown to reflect the expression level of FGF5 in HUVECs (n ¼ 3). (*: p < 0.05).

Article Snippet: The lung tissue from each group was made into paraffin slices, which were successively treated with dewaxing, Citrate Antigen Retrieval Solution (PH 6.0) to retrieve the antigen inmicrowave, the solution of 3% hydrogen peroxide to block endogenous peroxidase, 3% bovine serum albumin for 30min, and anti-FGF5 antibody (1:200, Proteintech, China) overnight.

Techniques: Staining, Immunohistochemical staining, Expressing

Journal: Biochemical and biophysical research communications

Article Title: FGF5 alleviated acute lung injury via AKT signal pathway in endothelial cells.

doi: 10.1016/j.bbrc.2022.09.112

Figure Lengend Snippet: Fig. 2. Overexpression of FGF5 ameliorated the pyroptosis level of HUVECs induced by LPS. AeB: Representative protein bands and statistical bar graph were shown to reflect FGF5 expression level in HUVECs with the FGF5 overexpression plasmid (n ¼ 3). C: Representative protein bands and statistical bar graph were applied to show the level of proteins which were related to pyroptosis (n ¼ 3). D: Representative images of flow cytometry and its statistical analysis bar graph (n ¼ 3). FeG: Representative images of TUNEL staining in HUVECs and its statistical analysis bar graph (n ¼ 3). (*: p < 0.05).

Article Snippet: The lung tissue from each group was made into paraffin slices, which were successively treated with dewaxing, Citrate Antigen Retrieval Solution (PH 6.0) to retrieve the antigen inmicrowave, the solution of 3% hydrogen peroxide to block endogenous peroxidase, 3% bovine serum albumin for 30min, and anti-FGF5 antibody (1:200, Proteintech, China) overnight.

Techniques: Over Expression, Expressing, Plasmid Preparation, Cytometry, TUNEL Assay, Staining

Journal: Biochemical and biophysical research communications

Article Title: FGF5 alleviated acute lung injury via AKT signal pathway in endothelial cells.

doi: 10.1016/j.bbrc.2022.09.112

Figure Lengend Snippet: Fig. 3. Overexpression of FGF5 alleviated the pathological changes of lung stimulated by LPS. A, C: Representative images of HE staining and the corresponding evaluation of lung injuries. B, D: Immunofluorescence staining of lungs for F4/80 and its statistical analysis bar graph (n ¼ 4). E: Statistical analysis of lung wet/dry ratio in different groups (n ¼ 4). (*: p < 0.05).

Article Snippet: The lung tissue from each group was made into paraffin slices, which were successively treated with dewaxing, Citrate Antigen Retrieval Solution (PH 6.0) to retrieve the antigen inmicrowave, the solution of 3% hydrogen peroxide to block endogenous peroxidase, 3% bovine serum albumin for 30min, and anti-FGF5 antibody (1:200, Proteintech, China) overnight.

Techniques: Over Expression, Staining

Journal: Biochemical and biophysical research communications

Article Title: FGF5 alleviated acute lung injury via AKT signal pathway in endothelial cells.

doi: 10.1016/j.bbrc.2022.09.112

Figure Lengend Snippet: Fig. 4. FGF5 overexpression in HUVECs activated AKT signal pathway. AeE: Representative images of protein bands and its statistical analysis (n ¼ 3e4). (*: p < 0.05).

Article Snippet: The lung tissue from each group was made into paraffin slices, which were successively treated with dewaxing, Citrate Antigen Retrieval Solution (PH 6.0) to retrieve the antigen inmicrowave, the solution of 3% hydrogen peroxide to block endogenous peroxidase, 3% bovine serum albumin for 30min, and anti-FGF5 antibody (1:200, Proteintech, China) overnight.

Techniques: Over Expression

Journal: Biochemical and biophysical research communications

Article Title: FGF5 alleviated acute lung injury via AKT signal pathway in endothelial cells.

doi: 10.1016/j.bbrc.2022.09.112

Figure Lengend Snippet: Fig. 5. The protection of FGF5 against pyroptosis was compromised with the inhibition of AKT signal. AeG: Representative images of protein bands and its statistical analysis (n ¼ 3e4). (*: p < 0.05).

Article Snippet: The lung tissue from each group was made into paraffin slices, which were successively treated with dewaxing, Citrate Antigen Retrieval Solution (PH 6.0) to retrieve the antigen inmicrowave, the solution of 3% hydrogen peroxide to block endogenous peroxidase, 3% bovine serum albumin for 30min, and anti-FGF5 antibody (1:200, Proteintech, China) overnight.

Techniques: Inhibition

Journal: Cell Stem Cell

Article Title: The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation

doi: 10.1016/j.stem.2018.12.001

Figure Lengend Snippet:

Article Snippet: FGF5 , TaqMan , Mm00438918_m1.

Techniques: Virus, Recombinant, SYBR Green Assay, Staining, RNA Amplification, Reverse Transcription, Flow Cytometry, Bicinchoninic Acid Protein Assay, Plasmid Preparation, Knock-In, shRNA, Software