Journal: Cancers

Article Title: Assessment of Ovarian Tumor Growth in Wild-Type and Lumican-Deficient Mice: Insights Using Infrared Spectral Imaging, Histopathology, and Immunohistochemistry

doi: 10.3390/cancers13235950

Figure Lengend Snippet: Analysis of collagen organization in ovarian tumor sections of wild-type and lumican-deficient mice. ( a , b ) Representative microphotographs of s.c. allograft sections stained with HES (top panel, original magnification 20×, scale bar 500 µm) in Lum +/+ ( a ) and Lum −/− mice ( b ); ( c – f ) Picrosirius red and SHG image analyses of ovarian allografts in tumors implanted in Lum +/+ mice ( c , d ) and in tumors from Lum −/− mice ( e , f ); ( c , e ) Collagen SHG images from ID8 ovarian tumors (original magnification 20×); ( d , f ) Ovarian tumor sections stained with Picrosirius red and viewed under widefield cross-polar optics (original magnification 20×, scale bar 50 µm). Birefringence of collagen fibers allows distinction between type I (red) and type III (green) collagens; ( g ) Analysis of collagen fibers intensity by SHG in tumors and healthy tissues present in each section (mean ± SD, ns: not significant); ( h ) Analysis of tumor ECM collagen organization from images derived from Gabor filtering and FFT, processed on Picrosirius red images (mean ± SD, ns: not significant); ( i ) Quantification on Picrosirius red stained sections of the relative distribution of red pixels (corresponding to type I collagen) in tumor ECM of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05); ( j ) Quantification on Picrosirius red stained sections of the relative distribution of green pixels (corresponding to type III collagen) within tumors of Lum +/+ and Lum −/− sections (mean ± SD, * p < 0.05).

Article Snippet: To assess the basketweave structure of collagen, an innovative bioimaging approach combining Fast Fourier Transform (FFT) with Gabor filtering was applied [ ].

Techniques: Staining, Derivative Assay