Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 1 | Decreased REV-ERBα protein abundance and increased protein levels of COL1A1 and LOX in IPF lungs compared to healthy control. Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc. Healthy controls contained 100% normal lung architecture with 85% alveoli surface area. IPF patient samples contained at least 50% lesion surface area. The protein abundance of REV-ERBα, COL1A1, and LOX were visualized and

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Quantitative Proteomics, Control

Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 7 | IAV infection induced dysregulation of profibrotic progression exa- cerbated in Rev-erbα Het mice. WT and Rev-erbα Het mice (equal number of male and female) infected (103 PFU/mouse) with IAV for 15 days, and lungs were sepa- rated for RNA/protein isolation, or fixed with 10% formalin for FFPE sections. a The protein abundance of COL1A2, VIM and activated LOX were measured by western blot. Representative blot images were shown. Different targets were run on the same membrane: COL1A2, VIM and activated LOX were probed in the same membrane and β-ACTIN was used as an endogenous control (n = 5–6 mice per group). b The localizations of COL1A1 and LOX were determined by

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Infection, Isolation, Quantitative Proteomics, Western Blot, Membrane, Control