Journal: Frontiers in Pharmacology

Article Title: Endoplasmic reticulum stress aggravates ferroptosis via PERK/ATF4/HSPA5 pathway in UUO-induced renal fibrosis

doi: 10.3389/fphar.2025.1545972

Figure Lengend Snippet: HSPA5 mediated TGF-β1-induced Ferroptosis and EMT in HK-2 cells. (A) Cell viability measured via an MTT assay exhibited a noticeable elevation in cell death of HK-2 cells exposed to TGF-β1 (10 ng/mL) in a dose-dependent manner of Fer-1. (B–E) The MDA, GSH, GSSG, and iron level were detected in each group (n = 5, *P < 0.05, **P < 0.05) (F, I) C11-BODIPY 581/591 was applied to detect lipid peroxidase in HK-2 cells treated with TGF-β1 in combination with Fer-1. (G–H) Representative blots of Col1a1, E-cad, α-SMA, GPX4 and HSPA5 expression (n = 5). (J–K) The GSH and MDA levels were detected in each group (n = 5, *P < 0.05, **P < 0.05). The results are expressed as the mean ± standard error of the mean (S.E.M.).

Article Snippet: Moreover, prior to TGF-β stimulation, cells in certain experimental groups were pre-exposed to Ferrostatin-1 (Fer-1) (1–100 μM, HY-100579, MedChemExpress, United States) or TUDCA (200 μM, HY-19696, MedChemExpress, United States).

Techniques: MTT Assay, Expressing

Journal: Immunity, Inflammation and Disease

Article Title: Kaempferol Attenuates Oxidative Stress‐Induced Injury in Gastric Mucosal Cells by Activating Nrf2/GPX4 Axis to Inhibit Ferroptosis

doi: 10.1002/iid3.70352

Figure Lengend Snippet: KAE alleviates oxidative stress and inhibits ferroptosis in H₂O₂‐treated GES‐1 cells. Con: control group; Model: H₂O₂‐treated group; Model + KAE: KAE‐treated group; Model + Fer‐1: ferrostatin‐1‐treated group. (A) Intracellular ROS levels measured by DCFH‐DA fluorescent probe. (B) Lipid peroxidation evaluated by BODIPY‐C11 staining. (C) MDA content determined by biochemical assay. (D) GSH/GSSG ratio reflecting antioxidant capacity. (E) Intracellular Fe²⁺ levels measured by colorimetric assay. (F) Western blot analysis of GPX4, SLC7A11, and ACSL4 protein levels. GAPDH was used as a loading control. Data are expressed as mean ± SD ( n = 3). Scale bar = 20 μm. Data are presented as mean ± SD ( n = 3 independent experiments). ** p < 0.01, *** p < 0.001 versus Con group; # p < 0.05, ## p < 0.01 versus Model group.

Article Snippet: Prior to H2O2 stimulation, cells were pretreated with various concentrations of KAE (0, 5, 10, 20, 40 μM; MCE, USA) for 2 h. In certain groups, the ferroptosis inhibitor Ferrostatin‐1 (Fer‐1, 1 μM, MCE) or the Nrf2 inhibitor ML385 (5 μM, MCE) was co‐incubated to verify the underlying mechanisms.

Techniques: Control, Staining, Colorimetric Assay, Western Blot