Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Schematic diagram for sample preparation of bulk stool (A) and FecalSwab (B) methods.

Article Snippet: Rojas et al. ( ) enhance our findings, as the authors evaluated the compatibility of the FecalSwab with additional pathogens ( Yersinia enterocolitica , enterotoxigenic Escherichia coli , Vibrio spp., and Plesiomonas shigelloides ) using the BD Max extended EBP panels; however, there are still several pathogens, especially intestinal parasites, that require investigation.

Techniques: Sample Prep

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Evaluation of various FecalSwab sample volumes (10 and 50 μl) to achieve equivalent detection to the BD Max standard-of-care (bulk stool) protocols for each EVP and EBP pathogen target. Standard of care recommends 5 μl of unpreserved stool for the EVP assay and 10 μl of specimen preserved in Cary-Blair for the EBP assay. Bars are representative of a single specimen positive for each target presented as mean C T values of triplicates ± standard deviation (SD). Comparison in molecular detection ( C T values) between bulk stool and 10-μl FecalSwab inoculums for each pathogen target were significant (**, P < 0.01). Equivalent detection to bulk stool was found for FecalSwab volumes of 50 μl ( P > 0.05) for all targets with the exception of sapovirus (*, P < 0.05).

Article Snippet: Rojas et al. ( ) enhance our findings, as the authors evaluated the compatibility of the FecalSwab with additional pathogens ( Yersinia enterocolitica , enterotoxigenic Escherichia coli , Vibrio spp., and Plesiomonas shigelloides ) using the BD Max extended EBP panels; however, there are still several pathogens, especially intestinal parasites, that require investigation.

Techniques: Standard Deviation

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Clinical evaluation of the FecalSwab as a specimen type for the BD Max enteric viral and enteric bacterial panels

Article Snippet: Rojas et al. ( ) enhance our findings, as the authors evaluated the compatibility of the FecalSwab with additional pathogens ( Yersinia enterocolitica , enterotoxigenic Escherichia coli , Vibrio spp., and Plesiomonas shigelloides ) using the BD Max extended EBP panels; however, there are still several pathogens, especially intestinal parasites, that require investigation.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Effect of FecalSwab storage time and temperature on the molecular detection of viral and bacterial enteropathogens in stool specimens. FecalSwab samples were prepared from clinical specimens positive for each pathogen target and stored at 4°C (A), 22°C (B), and 35°C (C) for 14 days. Samples were collected from each FecalSwab at baseline and at days 1, 2, 7, and 14. Cycle threshold ( C T ) values were determined using the BD Max EVP and EBP assays. Bars are representative of experimental triplicates and are presented as mean Δ C T values ± SD from baseline.

Article Snippet: Rojas et al. ( ) enhance our findings, as the authors evaluated the compatibility of the FecalSwab with additional pathogens ( Yersinia enterocolitica , enterotoxigenic Escherichia coli , Vibrio spp., and Plesiomonas shigelloides ) using the BD Max extended EBP panels; however, there are still several pathogens, especially intestinal parasites, that require investigation.

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Schematic diagram for sample preparation of bulk stool (A) and FecalSwab (B) methods.

Article Snippet: In this study, we evaluated the FecalSwab as a specimen type for the BD Max system using the viral and bacterial enteric panels (BD Diagnostics, Baltimore, MD, USA).

Techniques: Sample Prep

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Evaluation of various FecalSwab sample volumes (10 and 50 μl) to achieve equivalent detection to the BD Max standard-of-care (bulk stool) protocols for each EVP and EBP pathogen target. Standard of care recommends 5 μl of unpreserved stool for the EVP assay and 10 μl of specimen preserved in Cary-Blair for the EBP assay. Bars are representative of a single specimen positive for each target presented as mean C T values of triplicates ± standard deviation (SD). Comparison in molecular detection ( C T values) between bulk stool and 10-μl FecalSwab inoculums for each pathogen target were significant (**, P < 0.01). Equivalent detection to bulk stool was found for FecalSwab volumes of 50 μl ( P > 0.05) for all targets with the exception of sapovirus (*, P < 0.05).

Article Snippet: In this study, we evaluated the FecalSwab as a specimen type for the BD Max system using the viral and bacterial enteric panels (BD Diagnostics, Baltimore, MD, USA).

Techniques: Standard Deviation

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Clinical evaluation of the FecalSwab as a specimen type for the BD Max enteric viral and enteric bacterial panels

Article Snippet: In this study, we evaluated the FecalSwab as a specimen type for the BD Max system using the viral and bacterial enteric panels (BD Diagnostics, Baltimore, MD, USA).

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of the FecalSwab for Stool Specimen Storage and Molecular Detection of Enteropathogens on the BD Max System

doi: 10.1128/JCM.00178-20

Figure Lengend Snippet: Effect of FecalSwab storage time and temperature on the molecular detection of viral and bacterial enteropathogens in stool specimens. FecalSwab samples were prepared from clinical specimens positive for each pathogen target and stored at 4°C (A), 22°C (B), and 35°C (C) for 14 days. Samples were collected from each FecalSwab at baseline and at days 1, 2, 7, and 14. Cycle threshold ( C T ) values were determined using the BD Max EVP and EBP assays. Bars are representative of experimental triplicates and are presented as mean Δ C T values ± SD from baseline.

Article Snippet: In this study, we evaluated the FecalSwab as a specimen type for the BD Max system using the viral and bacterial enteric panels (BD Diagnostics, Baltimore, MD, USA).

Techniques: