Journal: Nature Communications

Article Title: Pyridoxine induces glutathione synthesis via PKM2-mediated Nrf2 transactivation and confers neuroprotection

doi: 10.1038/s41467-020-14788-x

Figure Lengend Snippet: a GSH levels in astrocytes and neurons after treatment with 0, 5, 10, 20, 40, or 80 µM cabergoline for 24 h. b GSH levels in astrocytes and neurons after treatment with 10 µM cabergoline for 6, 12, 18, or 24 h. c GSH levels in astrocytes treated with 10 μM cabergoline for 12 h after pretreatment with SCH23390 (10 μM) or sulpiride (10 μM). d GSH levels in astrocytes from wild-type mice or Drd2 -knockout mice after quinpirole (10 μM), quinelorane (20 μM), or bromocriptine (40 μM) treatment for 12 h. Six independent experiments per condition in a – d . e Striatal GSH levels in Drd2 flox/flox mice and Drd2 hGFAP cKO mice after 5 mg kg −1 quinpirole administration for 7 days. n = 6 mice per group. f Representative dot plots of ACSA-2 labeling of astrocytes (gated in blue circles) collected after microbead kit separation. g , h Gene set enrichment analysis of upregulated pathways ( g ) and heat map of the expression of Nrf2 targets (h) identified by RNA sequencing in astrocytes from mice after quinpirole administration. i Astrocytes were treated with 10 μM quinpirole, and Nrf2 target expression was determined by qRT-PCR. Three independent experiments per condition. j , k Immunoblotting with actin as a loading control ( j ) and GSH levels ( k ) in wild-type and Nrf2 -null astrocytes after 10 μM quinpirole treatment. Immunoblotting: three independent experiments; GSH assay: six independent experiments. l Diagram of the Gclc and Gclm promoters showing the locations of the different fragments tested. The numbers indicate the base pairs upstream of the transcriptional start site. m , n ChIP assays showing enrichment of the Gclc (m) and Gclm ( n ) promoters in DNA isolated from astrocytes treated with 10 μM quinpirole and precipitated with anti-Nrf2 antibodies. Three independent experiments per condition. Data are presented as the mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001, NS, not significant. One-way ANOVA with Tukey’s multiple comparisons test ( c ), two-way ANOVA with Dunnett’s multiple comparisons test ( a , b and d ) or with Sidak’s multiple comparisons test ( e , k ), Student’s two-tailed unpaired t -test ( i , m and n ). Source data are provided as a Source Data file.

Article Snippet: Cabergoline (HY-15296), bromocriptine (HY-12705A), SCH23390 (HY-19545A), DASA-58 (HY-19330), and pyridoxine (HY-B1328) were purchased from MedChemExpress.

Techniques: Knock-Out, Labeling, Expressing, RNA Sequencing Assay, Quantitative RT-PCR, Western Blot, Control, GSH Assay, Isolation, Two Tailed Test

Journal: Scientific Reports

Article Title: Label-free high-throughput screening assay for the identification of norepinephrine transporter (NET/SLC6A2) inhibitors

doi: 10.1038/s41598-021-91700-7

Figure Lengend Snippet: Inhibitory potency (pIC 50 ) values of NET inhibitors determined in the TRACT assay and fluorescent substrate uptake assay.

Article Snippet: GBR12909 dihydrochloride and reboxetine mesylate were purchased from Toronto Research Chemicals (Toronto, ON, Canada).

Techniques:

Journal: Data in Brief

Article Title: Proteomics dataset of liver tissue from spinal muscular atrophy, heterozygous, and wild-type mice, enabling pathway identification

doi: 10.1016/j.dib.2026.112632

Figure Lengend Snippet: Validation of proteomics-identified protein target with Western blot. ( A ) Western blot analysis of RIPA-extracted WT, HET, and SMA liver samples (n = 3 per group), using antibodies against ferrochelatase (FECH), survival motor neuron (SMN) protein and Vinculin (which served as loading control). ( B ) Quantification of FECH normalized to Vinculin, expressed as fold change relative to WT. Bars represent ± SD. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test (ns = not significant, ****p < 0.0001).

Article Snippet: The blot was incubated for SMN (same as above), FECH (rabbit, proteintech, Cat#: 14466-1-AP, dilution 1:1000), and vinculin (rabbit, Cat#:129002, Abcam, dilution 1:1000) served as the loading control.

Techniques: Biomarker Discovery, Western Blot, Control