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R&D Systems trkb fc chimera proteins
Figure 8 | TRPV1-induced excitatory synaptogenesis requires calcium influx, NGF and BDNF. (a) Immunostain of rat hippocampal cultures with TRPV1 and BDNF. (b) Quantitation of BDNF signal in somas of TRPV1-expressing neurons normalized to surrounding non-TRPV1-expressing cells (n ¼ 15 images from three cultures; error ¼ s.e.m., significance determined by unpaired Student’s t-test with Welch’s correction, **Po0.01). (c) Immunostain of WT and TRPV1 knockout mouse cultures with the C-terminal TRPV1 antibody (which detects a remainins splice isoform in the TRPV1 knockouts and marks TRPV1-expressing cells—Fig. 1e,f), BDNF, and DAPI. (d) Quantitation of BDNF puncta/mm (left panel) and BDNF puncta intensity (right panel) on OLM neurons marked by the C-terminal TRPV1 antibody, in WT and TRPV1 knockout cultures, normalized to WT. (e) Immunostains of TRPV1, vGluT and MAP2 in cultures in control conditions, and in cultures treated with capsaicin in the presence of absence of 2 mM EGTA to block calcium influx, 1 mg ml 1 TrkA-Fc to scavenge NGF, or 0.4mg ml 1 <t>TrkB-Fc</t> to scavenge BDNF. (f) Quantitation of excitatory synapse number (vGluT puncta number) on TRPV1-expressing hippocampal neurons in the indicated conditions. Images used for quantitation were: control n ¼ 28, 1 mM cap. n ¼ 14, 2 mM EGTA n ¼ 21, 2 mM EGTA þ 1 mM cap. n ¼ 21, 1 mgml 1
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Figure 8 | TRPV1-induced excitatory synaptogenesis requires calcium influx, NGF and BDNF. (a) Immunostain of rat hippocampal cultures with TRPV1 and BDNF. (b) Quantitation of BDNF signal in somas of TRPV1-expressing neurons normalized to surrounding non-TRPV1-expressing cells (n ¼ 15 images from three cultures; error ¼ s.e.m., significance determined by unpaired Student’s t-test with Welch’s correction, **Po0.01). (c) Immunostain of WT and TRPV1 knockout mouse cultures with the C-terminal TRPV1 antibody (which detects a remainins splice isoform in the TRPV1 knockouts and marks TRPV1-expressing cells—Fig. 1e,f), BDNF, and DAPI. (d) Quantitation of BDNF puncta/mm (left panel) and BDNF puncta intensity (right panel) on OLM neurons marked by the C-terminal TRPV1 antibody, in WT and TRPV1 knockout cultures, normalized to WT. (e) Immunostains of TRPV1, vGluT and MAP2 in cultures in control conditions, and in cultures treated with capsaicin in the presence of absence of 2 mM EGTA to block calcium influx, 1 mg ml 1 TrkA-Fc to scavenge NGF, or 0.4mg ml 1 <t>TrkB-Fc</t> to scavenge BDNF. (f) Quantitation of excitatory synapse number (vGluT puncta number) on TRPV1-expressing hippocampal neurons in the indicated conditions. Images used for quantitation were: control n ¼ 28, 1 mM cap. n ¼ 14, 2 mM EGTA n ¼ 21, 2 mM EGTA þ 1 mM cap. n ¼ 21, 1 mgml 1
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Figure 8 | TRPV1-induced excitatory synaptogenesis requires calcium influx, NGF and BDNF. (a) Immunostain of rat hippocampal cultures with TRPV1 and BDNF. (b) Quantitation of BDNF signal in somas of TRPV1-expressing neurons normalized to surrounding non-TRPV1-expressing cells (n ¼ 15 images from three cultures; error ¼ s.e.m., significance determined by unpaired Student’s t-test with Welch’s correction, **Po0.01). (c) Immunostain of WT and TRPV1 knockout mouse cultures with the C-terminal TRPV1 antibody (which detects a remainins splice isoform in the TRPV1 knockouts and marks TRPV1-expressing cells—Fig. 1e,f), BDNF, and DAPI. (d) Quantitation of BDNF puncta/mm (left panel) and BDNF puncta intensity (right panel) on OLM neurons marked by the C-terminal TRPV1 antibody, in WT and TRPV1 knockout cultures, normalized to WT. (e) Immunostains of TRPV1, vGluT and MAP2 in cultures in control conditions, and in cultures treated with capsaicin in the presence of absence of 2 mM EGTA to block calcium influx, 1 mg ml 1 TrkA-Fc to scavenge NGF, or 0.4mg ml 1 <t>TrkB-Fc</t> to scavenge BDNF. (f) Quantitation of excitatory synapse number (vGluT puncta number) on TRPV1-expressing hippocampal neurons in the indicated conditions. Images used for quantitation were: control n ¼ 28, 1 mM cap. n ¼ 14, 2 mM EGTA n ¼ 21, 2 mM EGTA þ 1 mM cap. n ¼ 21, 1 mgml 1
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R&D Systems recombinant her2 fc chimera protein
Figure 8 | TRPV1-induced excitatory synaptogenesis requires calcium influx, NGF and BDNF. (a) Immunostain of rat hippocampal cultures with TRPV1 and BDNF. (b) Quantitation of BDNF signal in somas of TRPV1-expressing neurons normalized to surrounding non-TRPV1-expressing cells (n ¼ 15 images from three cultures; error ¼ s.e.m., significance determined by unpaired Student’s t-test with Welch’s correction, **Po0.01). (c) Immunostain of WT and TRPV1 knockout mouse cultures with the C-terminal TRPV1 antibody (which detects a remainins splice isoform in the TRPV1 knockouts and marks TRPV1-expressing cells—Fig. 1e,f), BDNF, and DAPI. (d) Quantitation of BDNF puncta/mm (left panel) and BDNF puncta intensity (right panel) on OLM neurons marked by the C-terminal TRPV1 antibody, in WT and TRPV1 knockout cultures, normalized to WT. (e) Immunostains of TRPV1, vGluT and MAP2 in cultures in control conditions, and in cultures treated with capsaicin in the presence of absence of 2 mM EGTA to block calcium influx, 1 mg ml 1 TrkA-Fc to scavenge NGF, or 0.4mg ml 1 <t>TrkB-Fc</t> to scavenge BDNF. (f) Quantitation of excitatory synapse number (vGluT puncta number) on TRPV1-expressing hippocampal neurons in the indicated conditions. Images used for quantitation were: control n ¼ 28, 1 mM cap. n ¼ 14, 2 mM EGTA n ¼ 21, 2 mM EGTA þ 1 mM cap. n ¼ 21, 1 mgml 1
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Figure 8 | TRPV1-induced excitatory synaptogenesis requires calcium influx, NGF and BDNF. (a) Immunostain of rat hippocampal cultures with TRPV1 and BDNF. (b) Quantitation of BDNF signal in somas of TRPV1-expressing neurons normalized to surrounding non-TRPV1-expressing cells (n ¼ 15 images from three cultures; error ¼ s.e.m., significance determined by unpaired Student’s t-test with Welch’s correction, **Po0.01). (c) Immunostain of WT and TRPV1 knockout mouse cultures with the C-terminal TRPV1 antibody (which detects a remainins splice isoform in the TRPV1 knockouts and marks TRPV1-expressing cells—Fig. 1e,f), BDNF, and DAPI. (d) Quantitation of BDNF puncta/mm (left panel) and BDNF puncta intensity (right panel) on OLM neurons marked by the C-terminal TRPV1 antibody, in WT and TRPV1 knockout cultures, normalized to WT. (e) Immunostains of TRPV1, vGluT and MAP2 in cultures in control conditions, and in cultures treated with capsaicin in the presence of absence of 2 mM EGTA to block calcium influx, 1 mg ml 1 TrkA-Fc to scavenge NGF, or 0.4mg ml 1 <t>TrkB-Fc</t> to scavenge BDNF. (f) Quantitation of excitatory synapse number (vGluT puncta number) on TRPV1-expressing hippocampal neurons in the indicated conditions. Images used for quantitation were: control n ¼ 28, 1 mM cap. n ¼ 14, 2 mM EGTA n ¼ 21, 2 mM EGTA þ 1 mM cap. n ¼ 21, 1 mgml 1
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Image Search Results


Journal: Molecular Cell

Article Title: Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells

doi: 10.1016/j.molcel.2022.04.033

Figure Lengend Snippet:

Article Snippet: A549 ACE2 & TMPRSS2 Cells , a549-hace2tpsa , Invivogen.

Techniques: Virus, Variant Assay, Clinical Proteomics, Recombinant, Microscopy, Plasmid Preparation, Mutagenesis, Construct, Software

Figure 8 | TRPV1-induced excitatory synaptogenesis requires calcium influx, NGF and BDNF. (a) Immunostain of rat hippocampal cultures with TRPV1 and BDNF. (b) Quantitation of BDNF signal in somas of TRPV1-expressing neurons normalized to surrounding non-TRPV1-expressing cells (n ¼ 15 images from three cultures; error ¼ s.e.m., significance determined by unpaired Student’s t-test with Welch’s correction, **Po0.01). (c) Immunostain of WT and TRPV1 knockout mouse cultures with the C-terminal TRPV1 antibody (which detects a remainins splice isoform in the TRPV1 knockouts and marks TRPV1-expressing cells—Fig. 1e,f), BDNF, and DAPI. (d) Quantitation of BDNF puncta/mm (left panel) and BDNF puncta intensity (right panel) on OLM neurons marked by the C-terminal TRPV1 antibody, in WT and TRPV1 knockout cultures, normalized to WT. (e) Immunostains of TRPV1, vGluT and MAP2 in cultures in control conditions, and in cultures treated with capsaicin in the presence of absence of 2 mM EGTA to block calcium influx, 1 mg ml 1 TrkA-Fc to scavenge NGF, or 0.4mg ml 1 TrkB-Fc to scavenge BDNF. (f) Quantitation of excitatory synapse number (vGluT puncta number) on TRPV1-expressing hippocampal neurons in the indicated conditions. Images used for quantitation were: control n ¼ 28, 1 mM cap. n ¼ 14, 2 mM EGTA n ¼ 21, 2 mM EGTA þ 1 mM cap. n ¼ 21, 1 mgml 1

Journal: Nature communications

Article Title: TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus.

doi: 10.1038/ncomms15878

Figure Lengend Snippet: Figure 8 | TRPV1-induced excitatory synaptogenesis requires calcium influx, NGF and BDNF. (a) Immunostain of rat hippocampal cultures with TRPV1 and BDNF. (b) Quantitation of BDNF signal in somas of TRPV1-expressing neurons normalized to surrounding non-TRPV1-expressing cells (n ¼ 15 images from three cultures; error ¼ s.e.m., significance determined by unpaired Student’s t-test with Welch’s correction, **Po0.01). (c) Immunostain of WT and TRPV1 knockout mouse cultures with the C-terminal TRPV1 antibody (which detects a remainins splice isoform in the TRPV1 knockouts and marks TRPV1-expressing cells—Fig. 1e,f), BDNF, and DAPI. (d) Quantitation of BDNF puncta/mm (left panel) and BDNF puncta intensity (right panel) on OLM neurons marked by the C-terminal TRPV1 antibody, in WT and TRPV1 knockout cultures, normalized to WT. (e) Immunostains of TRPV1, vGluT and MAP2 in cultures in control conditions, and in cultures treated with capsaicin in the presence of absence of 2 mM EGTA to block calcium influx, 1 mg ml 1 TrkA-Fc to scavenge NGF, or 0.4mg ml 1 TrkB-Fc to scavenge BDNF. (f) Quantitation of excitatory synapse number (vGluT puncta number) on TRPV1-expressing hippocampal neurons in the indicated conditions. Images used for quantitation were: control n ¼ 28, 1 mM cap. n ¼ 14, 2 mM EGTA n ¼ 21, 2 mM EGTA þ 1 mM cap. n ¼ 21, 1 mgml 1

Article Snippet: Recombinant Human TrkA Fc and TrkB Fc Chimera Proteins were from R & D Systems.

Techniques: Quantitation Assay, Expressing, Knock-Out, Control, Blocking Assay