fast sybr green master mix Thermo Fisher Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher sybr master mix
    Assessment of BRCA1 loss (A) Mutation screening showing the abnormal denaturing high performance liquid chromatography profile corresponding to the 1351delAT mutation in tumor 223. The single blue line represents the electropherogram from a normal control, while the purple line represents the abnormal profile formed by the mutated exon 11c in tumor 223. (B) Direct <t>DNA</t> sequencing demonstrating the 185delAG mutation in tumor 283. Only the mutant allele is seen in the tumor because LOH is present. (C-E) Loss of heterozygosity (LOH) analysis using BRCA1-associated microsatellite markers visualized on an ABI Prism 3100 Genetic Analyzer, where LOH is defined as > 50% decrease in area under the curve when germline DNA (upper tracing) and tumor DNA (lower tracing) are compared. (C) The lack of LOH in tumor 240 demonstrated using microsatellite marker D17S1185, (D) LOH in tumor 283 demonstrated using microsatellite marker D17S855. (E) Microsatellite instability demonstrated in tumor 156 using microsatellite marker D17S1185. (F, G, H, and I) Methylation analysis of BRCA1 gene using fluorescence-based, quantitative, real-time PCR (TaqMan) using <t>SYBR</t> Green 1 as detection method. Two sets of primers, designed specifically for bisulfite converted DNA, were used: a methylated set for the BRCA1 gene and a reference set (MYOD1) to control for input DNA. Specificity of the reactions for methylated DNA were confirmed separately using human genomic DNA (unmethyated; F) and CpG methylated Jurkat genomic DNA (methylated; G), respectively. H and I show representative examples of results from assessment of BRCA1 loss through promoter hypermethyation. Tumor 178 shows only unmethylated BRCA1 promoter, while tumor 345 shows evidence of BRCA1 promoter hypermethylation.
    Sybr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr master mix/product/Thermo Fisher
    Average 99 stars, based on 10358 article reviews
    Price from $9.99 to $1999.99
    sybr master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher taqman fast advanced master mix
    ASL Is Highly Expressed in the LC and Regulates TH Levels (A) Left: in situ hybridization with Asl anti-sense mRNA probe showing in purple Asl prominent expression in the LC. Right: scheme of brain stem coronal section is shown. LC region is highlighted in purple (image adapted from The Mouse Brain Atlas). (B) Immunostaining of mouse brainstem for Asl (left, red), TH (center, green), and their merged co-localization (right). (C) Immunostaining of human brainstem for ASL (left, red), TH (center, green), and their merged co-localization (right). (D and E) Quantification of Asl mRNA (D) and TH mRNA (E) isolated by laser microdissection from the LC of Asl f/f ;TH Cre +/− and from Asl f/f control mice as measured by <t>RT-PCR</t> with specific <t>TaqMan</t> probes (n = 100 cells from 7 animals). (F) Immunohistochemistry quantification of TH protein normalized to cells number (n = 4 in each group). (G) Quantification of western blots for TH levels in LC regions taken by punch biopsies (n = 4 in each group). The bottom panels of (F) and (G) show representative images for each detection method, respectively. Data represent mean ± SEM ( ∗ p
    Taqman Fast Advanced Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taqman fast advanced master mix/product/Thermo Fisher
    Average 99 stars, based on 6206 article reviews
    Price from $9.99 to $1999.99
    taqman fast advanced master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Assessment of BRCA1 loss (A) Mutation screening showing the abnormal denaturing high performance liquid chromatography profile corresponding to the 1351delAT mutation in tumor 223. The single blue line represents the electropherogram from a normal control, while the purple line represents the abnormal profile formed by the mutated exon 11c in tumor 223. (B) Direct DNA sequencing demonstrating the 185delAG mutation in tumor 283. Only the mutant allele is seen in the tumor because LOH is present. (C-E) Loss of heterozygosity (LOH) analysis using BRCA1-associated microsatellite markers visualized on an ABI Prism 3100 Genetic Analyzer, where LOH is defined as > 50% decrease in area under the curve when germline DNA (upper tracing) and tumor DNA (lower tracing) are compared. (C) The lack of LOH in tumor 240 demonstrated using microsatellite marker D17S1185, (D) LOH in tumor 283 demonstrated using microsatellite marker D17S855. (E) Microsatellite instability demonstrated in tumor 156 using microsatellite marker D17S1185. (F, G, H, and I) Methylation analysis of BRCA1 gene using fluorescence-based, quantitative, real-time PCR (TaqMan) using SYBR Green 1 as detection method. Two sets of primers, designed specifically for bisulfite converted DNA, were used: a methylated set for the BRCA1 gene and a reference set (MYOD1) to control for input DNA. Specificity of the reactions for methylated DNA were confirmed separately using human genomic DNA (unmethyated; F) and CpG methylated Jurkat genomic DNA (methylated; G), respectively. H and I show representative examples of results from assessment of BRCA1 loss through promoter hypermethyation. Tumor 178 shows only unmethylated BRCA1 promoter, while tumor 345 shows evidence of BRCA1 promoter hypermethylation.

    Journal: BMC Cancer

    Article Title: Ovarian carcinomas with genetic and epigenetic BRCA1 loss have distinct molecular abnormalities

    doi: 10.1186/1471-2407-8-17

    Figure Lengend Snippet: Assessment of BRCA1 loss (A) Mutation screening showing the abnormal denaturing high performance liquid chromatography profile corresponding to the 1351delAT mutation in tumor 223. The single blue line represents the electropherogram from a normal control, while the purple line represents the abnormal profile formed by the mutated exon 11c in tumor 223. (B) Direct DNA sequencing demonstrating the 185delAG mutation in tumor 283. Only the mutant allele is seen in the tumor because LOH is present. (C-E) Loss of heterozygosity (LOH) analysis using BRCA1-associated microsatellite markers visualized on an ABI Prism 3100 Genetic Analyzer, where LOH is defined as > 50% decrease in area under the curve when germline DNA (upper tracing) and tumor DNA (lower tracing) are compared. (C) The lack of LOH in tumor 240 demonstrated using microsatellite marker D17S1185, (D) LOH in tumor 283 demonstrated using microsatellite marker D17S855. (E) Microsatellite instability demonstrated in tumor 156 using microsatellite marker D17S1185. (F, G, H, and I) Methylation analysis of BRCA1 gene using fluorescence-based, quantitative, real-time PCR (TaqMan) using SYBR Green 1 as detection method. Two sets of primers, designed specifically for bisulfite converted DNA, were used: a methylated set for the BRCA1 gene and a reference set (MYOD1) to control for input DNA. Specificity of the reactions for methylated DNA were confirmed separately using human genomic DNA (unmethyated; F) and CpG methylated Jurkat genomic DNA (methylated; G), respectively. H and I show representative examples of results from assessment of BRCA1 loss through promoter hypermethyation. Tumor 178 shows only unmethylated BRCA1 promoter, while tumor 345 shows evidence of BRCA1 promoter hypermethylation.

    Article Snippet: Samples (10 ng bisulfite-treated DNA) were run in triplicate containing 5 μL SYBR Green Master Mix (Applied Biosystems, Foster City, CA) and 5 pmol of each forward and reverse primer.

    Techniques: Mutagenesis, High Performance Liquid Chromatography, DNA Sequencing, Marker, Methylation, Fluorescence, Real-time Polymerase Chain Reaction, SYBR Green Assay

    The BAC NEUROG3-SeAP/EGFP transgenes are expressed in the developing pancreas. (A) Homologous recombination ( Yang et al., 1997 ) was used to replace the human neurogenin-3 coding sequence in the NEUROG3 BAC (RP11-343J3T) with two reporter genes, SeAP and EGFP, flanked on the 5′ end by the human β-globulin intron and the 3′ end by the SV40 polyadenylation signal, and separated by a viral IRES. (B) Levels of neurogenin-3 mRNA in mouse pancreas were measured by real-time TaqMan RT-PCR at the embryonic dates shown and in adult islets, and are expressed relative to levels of histone H3.3a mRNA. (C) Levels of the SeAP / EGF P mRNA in mouse pancreas were measured by real-time SYBR Green RT-PCR at the embryonic dates shown and are expressed relative to levels of mouse β-actin mRNA. (D) Tissue SeAP activity was measured in pancreas homogenates at the embryonic dates shown and is expressed relative to total protein. All data represent mean + s.e.m. from at least three independent experiments.

    Journal: Disease Models & Mechanisms

    Article Title: A mouse model for monitoring islet cell genesis and developing therapies for diabetes

    doi: 10.1242/dmm.002998

    Figure Lengend Snippet: The BAC NEUROG3-SeAP/EGFP transgenes are expressed in the developing pancreas. (A) Homologous recombination ( Yang et al., 1997 ) was used to replace the human neurogenin-3 coding sequence in the NEUROG3 BAC (RP11-343J3T) with two reporter genes, SeAP and EGFP, flanked on the 5′ end by the human β-globulin intron and the 3′ end by the SV40 polyadenylation signal, and separated by a viral IRES. (B) Levels of neurogenin-3 mRNA in mouse pancreas were measured by real-time TaqMan RT-PCR at the embryonic dates shown and in adult islets, and are expressed relative to levels of histone H3.3a mRNA. (C) Levels of the SeAP / EGF P mRNA in mouse pancreas were measured by real-time SYBR Green RT-PCR at the embryonic dates shown and are expressed relative to levels of mouse β-actin mRNA. (D) Tissue SeAP activity was measured in pancreas homogenates at the embryonic dates shown and is expressed relative to total protein. All data represent mean + s.e.m. from at least three independent experiments.

    Article Snippet: Quantification of SeAP /EGFP cDNA was performed with SYBR Green using Fast SYBR Master Mix (Applied Biosystems) and reported relative to levels of the cDNA encoding mouse β-actin.

    Techniques: BAC Assay, Homologous Recombination, Sequencing, Reverse Transcription Polymerase Chain Reaction, SYBR Green Assay, Activity Assay

    Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased PCR sensitivity for CC75-08 in the hydrolysis probe PCR. Analytical sensitivity equal for S . aureus (CCUG31966) in hydrolysis probe PCR and SybrGreen PCR. Analytical sensitivity increased for CC75 lineage/ S . argenteus (CC75-08) strains in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Journal: PLoS ONE

    Article Title: Introduction of a hydrolysis probe PCR assay for high-throughput screening of methicillin-resistant Staphylococcus aureus with the ability to include or exclude detection of Staphylococcus argenteus

    doi: 10.1371/journal.pone.0192782

    Figure Lengend Snippet: Increased stability using hydrolysis probe PCR. Cq values from PCR control collected during one year from hydrolysis probe PCR (a) and SybrGreen PCR (b) show increased stability in hydrolysis probe PCR.

    Article Snippet: Primary detection amplification conditions Amplification of the nuc gene using SybrGreen was carried out in a 20 μL reaction mix using 2x Fast SybrGreen Master Mix (ThermoFisher), 0,5 μM of each nuc -SG primer (Eurogentec, Seraing, Belgium) and 5 μL of DNA template.

    Techniques: Polymerase Chain Reaction

    HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Journal: Heart rhythm

    Article Title: HuR-mediated SCN5A mRNA stability reduces arrhythmic risk in heart failure

    doi: 10.1016/j.hrthm.2018.02.018

    Figure Lengend Snippet: HuR associates with SCN5A mRNA (A) Schematic representation of the SCN5A mRNA ARE sites in its 3′-UTR. The length of the mRNA is indicated by a kilo base (kb)-scale. (B) HuR binds to the SCN5A mRNA. After IP of RNA-protein complexes from cardiomyocytes using either anti-HuR antibody (Anti-HuR) or control IgG, RNA was isolated, and then, the cDNA was synthesized in a presence or absence of the reverse transcription enzyme. Two sets of primers to different regions of SCN5A were used to amplify SCN5A fragments. The PCR products were visualized with SYBR ™ Safe DNA Gel Stain in agarose gels. (C) qRT-PCR was performed to confirm the results in (B).

    Article Snippet: Quantitative real-time reverse-transcriptase polymerase chain reaction (qRT-PCR) was carried out using gene-specific primers, Fast SYBR® Green Master Mix and 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Isolation, Synthesized, Polymerase Chain Reaction, Staining, Quantitative RT-PCR

    Genotypic levels of total RpL 14 mRNA expression in groups of whole individuals. Amount of RpL 14 mRNA in adults and larvae relative to three normalization genes. Height of bars indicate total amount of RpL 14 mRNA based on SYBR green-based quantitative reverse-transcription PCR and each bar is split to represent the proportion of total RpL 14 expressed from the RpL 14 [r] gene in { Ud }86 (white) and the endogenous RpL 14 [+] gene (gray), based on gene-specific TaqMan probes. Error bars represent 1 standard error for three biological replicates.

    Journal: PLoS ONE

    Article Title: First Steps towards Underdominant Genetic Transformation of Insect Populations

    doi: 10.1371/journal.pone.0097557

    Figure Lengend Snippet: Genotypic levels of total RpL 14 mRNA expression in groups of whole individuals. Amount of RpL 14 mRNA in adults and larvae relative to three normalization genes. Height of bars indicate total amount of RpL 14 mRNA based on SYBR green-based quantitative reverse-transcription PCR and each bar is split to represent the proportion of total RpL 14 expressed from the RpL 14 [r] gene in { Ud }86 (white) and the endogenous RpL 14 [+] gene (gray), based on gene-specific TaqMan probes. Error bars represent 1 standard error for three biological replicates.

    Article Snippet: 1 µL of the resulting reaction was used as a template for qPCR, using TaqMan Fast Master Mix (Applied Biosystems) for determining the relative ratios of wild-type and rescue transcripts or SYBR Green Fast Master Mix (Applied Biosystems) for total RpL14 mRNA levels.

    Techniques: Expressing, SYBR Green Assay, Polymerase Chain Reaction

    ASL Is Highly Expressed in the LC and Regulates TH Levels (A) Left: in situ hybridization with Asl anti-sense mRNA probe showing in purple Asl prominent expression in the LC. Right: scheme of brain stem coronal section is shown. LC region is highlighted in purple (image adapted from The Mouse Brain Atlas). (B) Immunostaining of mouse brainstem for Asl (left, red), TH (center, green), and their merged co-localization (right). (C) Immunostaining of human brainstem for ASL (left, red), TH (center, green), and their merged co-localization (right). (D and E) Quantification of Asl mRNA (D) and TH mRNA (E) isolated by laser microdissection from the LC of Asl f/f ;TH Cre +/− and from Asl f/f control mice as measured by RT-PCR with specific TaqMan probes (n = 100 cells from 7 animals). (F) Immunohistochemistry quantification of TH protein normalized to cells number (n = 4 in each group). (G) Quantification of western blots for TH levels in LC regions taken by punch biopsies (n = 4 in each group). The bottom panels of (F) and (G) show representative images for each detection method, respectively. Data represent mean ± SEM ( ∗ p

    Journal: Cell Reports

    Article Title: ASL Metabolically Regulates Tyrosine Hydroxylase in the Nucleus Locus Coeruleus

    doi: 10.1016/j.celrep.2019.10.043

    Figure Lengend Snippet: ASL Is Highly Expressed in the LC and Regulates TH Levels (A) Left: in situ hybridization with Asl anti-sense mRNA probe showing in purple Asl prominent expression in the LC. Right: scheme of brain stem coronal section is shown. LC region is highlighted in purple (image adapted from The Mouse Brain Atlas). (B) Immunostaining of mouse brainstem for Asl (left, red), TH (center, green), and their merged co-localization (right). (C) Immunostaining of human brainstem for ASL (left, red), TH (center, green), and their merged co-localization (right). (D and E) Quantification of Asl mRNA (D) and TH mRNA (E) isolated by laser microdissection from the LC of Asl f/f ;TH Cre +/− and from Asl f/f control mice as measured by RT-PCR with specific TaqMan probes (n = 100 cells from 7 animals). (F) Immunohistochemistry quantification of TH protein normalized to cells number (n = 4 in each group). (G) Quantification of western blots for TH levels in LC regions taken by punch biopsies (n = 4 in each group). The bottom panels of (F) and (G) show representative images for each detection method, respectively. Data represent mean ± SEM ( ∗ p

    Article Snippet: Quantitative PCR was performed using SYBR green PCR master mix (Thermo Fisher scientific 4385612) or with TaqMan Real-Time PCR Master Mix (Thermo Fisher scientific 4444557).

    Techniques: In Situ Hybridization, Expressing, Immunostaining, Isolation, Laser Capture Microdissection, Mouse Assay, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Western Blot