Journal: Cancers

Article Title: Drug Repurposing to Identify a Synergistic High-Order Drug Combination to Treat Sunitinib-Resistant Renal Cell Carcinoma

doi: 10.3390/cancers13163978

Figure Lengend Snippet: Drug–drug interactions and efficacy in cancer treatment.

Article Snippet: Acetylsalicyl acid (HY-107831), disulfiram (HY-B0240), famotidine (HY-B0377), ivermectin (HY-15310), metformin (HY-17471A), parthenolide (HY-N0141) and verapamil (HY-A0064) were purchased through MedChemExpress (Monmouth Junction, NJ, USA).

Techniques: Inhibition, Reflux

Journal: Scientific Reports

Article Title: NanoBRET binding assay for histamine H 2 receptor ligands using live recombinant HEK293T cells

doi: 10.1038/s41598-020-70332-3

Figure Lengend Snippet: Representative isotherms from NanoBRET saturation binding experiments with 8 – 10 at the NLuc-hH 2 R, stably expressed in HEK293T cells. Non-specific binding was determined in presence of a 300-fold excess of famotidine. *: Indicated K d -values are results from single experiments. Error bars of total and non-specific binding represent SEM. The error of the specific binding was calculated according to the Gaussian law of error propagation. Each experiment was performed in triplicate (N = 3).

Article Snippet: Famotidine and ranitidine hydrochloride were from Tocris Bioscience (Ellisville, MO, USA).

Techniques: Binding Assay, Stable Transfection

Journal: Scientific Reports

Article Title: NanoBRET binding assay for histamine H 2 receptor ligands using live recombinant HEK293T cells

doi: 10.1038/s41598-020-70332-3

Figure Lengend Snippet: BRET-based specific binding kinetics of the fluorescent ligand 8 at the NLuc-hH 2 R, stably expressed in HEK293T cells. Left panel: Association of 8 (c = 50 nM) to the receptor; Right panel: Dissociation of 8 (c = 50 nM) after preincubation for 60 min. Dissociation was started by addition of famotidine (300-fold excess, c = 15 µM). Graphs show representative experiments (N = 4), each performed in triplicate. Error bars of specific binding represent propagated errors.

Article Snippet: Famotidine and ranitidine hydrochloride were from Tocris Bioscience (Ellisville, MO, USA).

Techniques: Binding Assay, Stable Transfection

Journal: Scientific Reports

Article Title: NanoBRET binding assay for histamine H 2 receptor ligands using live recombinant HEK293T cells

doi: 10.1038/s41598-020-70332-3

Figure Lengend Snippet: Binding data (p K i values) of histamine ( 1 ), cimetidine ( 2 ), ranitidine ( 3 ), famotidine ( 4 ), UR-Po444 ( 11 ) and UR-Po448 ( 12 ) determined at the human H 2 R in the NanoBRET binding assay and radioligand competition binding assays.

Article Snippet: Famotidine and ranitidine hydrochloride were from Tocris Bioscience (Ellisville, MO, USA).

Techniques: Binding Assay

Journal:

Article Title: Characterization of changes in mechanical responses to histamine in omental resistance arteries in pre-eclampsia

doi: 10.1038/sj.bjp.0703529

Figure Lengend Snippet: Actual tracings of the effect of famotidine (3 μM) on the relaxation induced by histamine (1 μM) in the presence of STA2 in endothelium-denuded strips from normotensive pregnant (a) and pre-eclamptic women (b). Each agent was applied as indicated by the bar. Famotidine (3 μM) was applied in the presence of histamine at the peak of the histamine-induced relaxation observed in the presence of STA2. The concentration of STA2 was 3 nM for the strip obtained from a normotensive pregnant woman and 1 nM for the strip from a pre-eclamptic woman.

Article Snippet: Famotidine was kindly provided by Yamanouchi Pharmaceutical Co. Ltd. (Tokyo, Japan).

Techniques: Concentration Assay, Stripping Membranes