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  • 86
    Becton Dickinson facsaria flow cytometer
    Cellular uptake of newly-synthesized hybrid NPs. Two million DCs were treated with 500 μg hybrid NPs, which were labeled with NBD (green) in the lipid layer and Alexa 647 (red) in the PLGA core, for 30, 60, and 120 min, respectively. The cellular uptake of the NPs were quantitatively and qualitatively studied using a BD <t>FACSARIA</t> flow cytometer and an Amnis ® ImageStream flow cytometer, respectively. (A) The percentage of cells that internalized NPs. (B) The NBD median intensity in the cells. (C) The Alexa 647 median intensity in the cells. (D) Representative microscopic images of cellular uptake of the hybrid NPs by the DCs.
    Facsaria Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facsaria flow cytometer/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facsaria flow cytometer - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    99
    Becton Dickinson flow cytometer
    Cellular uptake of newly-synthesized hybrid NPs. Two million DCs were treated with 500 μg hybrid NPs, which were labeled with NBD (green) in the lipid layer and Alexa 647 (red) in the PLGA core, for 30, 60, and 120 min, respectively. The cellular uptake of the NPs were quantitatively and qualitatively studied using a BD <t>FACSARIA</t> flow cytometer and an Amnis ® ImageStream flow cytometer, respectively. (A) The percentage of cells that internalized NPs. (B) The NBD median intensity in the cells. (C) The Alexa 647 median intensity in the cells. (D) Representative microscopic images of cellular uptake of the hybrid NPs by the DCs.
    Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flow cytometer/product/Becton Dickinson
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    flow cytometer - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    86
    Becton Dickinson facsaria iii flow cytometer
    API5 expression is increased in cancer stem-like cell-enriched populations. ( a ) Flow cytometry analysis of CD44 expression in CaSki/D b P0 and P3 cells. The bar graph depicts the percentage of CD44 (mean±s.d.). ( b ) Western blot analysis of expression of API5, FGF2 and NANOG in CaSki/D b P0 and P3 cells. ( c ) CD44 high or CD44 low cells were sorted from CaSki/D b P3 cells by using <t>FACSAria</t> <t>III</t> flow cytometer (left panel). Flow cytometry analysis of CD44 expression in CD44 high or CD44 low cells (right panel). ( d ) Western blot analysis of expression of API5, FGF2 and NANOG in CD44 high or CD44 low cells. ( e ) Flow cytometry analysis of CD44 in CaSki cells grown in monolayer cultures (monolayers) and spheres isolated from CaSki cells under suspension conditions (spheres). The bar graph depicts the percentage of CD44 (mean±s.d.). ( f ) Western blot analysis of expression of API5, FGF2 and NANOG in spheres and monolayers of CaSki cells. ( b , d , f ) Numbers below blots indicate the expression as measured by fold change.
    Facsaria Iii Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/facsaria iii flow cytometer/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    facsaria iii flow cytometer - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier


    Image Search Results


    Cellular uptake of newly-synthesized hybrid NPs. Two million DCs were treated with 500 μg hybrid NPs, which were labeled with NBD (green) in the lipid layer and Alexa 647 (red) in the PLGA core, for 30, 60, and 120 min, respectively. The cellular uptake of the NPs were quantitatively and qualitatively studied using a BD FACSARIA flow cytometer and an Amnis ® ImageStream flow cytometer, respectively. (A) The percentage of cells that internalized NPs. (B) The NBD median intensity in the cells. (C) The Alexa 647 median intensity in the cells. (D) Representative microscopic images of cellular uptake of the hybrid NPs by the DCs.

    Journal: Biomaterials

    Article Title: Paradox of PEGylation in fabricating hybrid nanoparticle-based nicotine vaccines

    doi: 10.1016/j.biomaterials.2018.08.015

    Figure Lengend Snippet: Cellular uptake of newly-synthesized hybrid NPs. Two million DCs were treated with 500 μg hybrid NPs, which were labeled with NBD (green) in the lipid layer and Alexa 647 (red) in the PLGA core, for 30, 60, and 120 min, respectively. The cellular uptake of the NPs were quantitatively and qualitatively studied using a BD FACSARIA flow cytometer and an Amnis ® ImageStream flow cytometer, respectively. (A) The percentage of cells that internalized NPs. (B) The NBD median intensity in the cells. (C) The Alexa 647 median intensity in the cells. (D) Representative microscopic images of cellular uptake of the hybrid NPs by the DCs.

    Article Snippet: Cell samples were immediately analyzed by a BD FACSARIA flow cytometer (BD, Franklin Lakes, NJ) and a Amnis® ImageStream® X Mark II flow cytometer (MilliporeSigma, Billerica, MA), respectively.

    Techniques: Synthesized, Labeling, Flow Cytometry, Cytometry

    Cellular uptake of stored hybrid NPs. Two million DCs were incubated with 500 μg hybrid NPs that underwent 30 days’ storage at 4 °C in PBS buffer for 120 min. The cellular uptake of the NPs which were labeled with Alexa 647 were quantitatively and qualitatively studied using a BD FACSARIA flow cytometer and an Amnis ® ImageStream flow cytometer, respectively. (A) Dots plot of cellular uptake of NPs. (B) The Alexa 647 median intensity in the DCs. (C) Representative microscopic images of cellular uptake of the hybrid NPs. *** in Fig. 4. A means the percentage of cells internalized NPs that contained more PEG was significantly higher than those with less PEG in the lipid layer. *** denotes a P-value that is less than 0.01.

    Journal: Biomaterials

    Article Title: Paradox of PEGylation in fabricating hybrid nanoparticle-based nicotine vaccines

    doi: 10.1016/j.biomaterials.2018.08.015

    Figure Lengend Snippet: Cellular uptake of stored hybrid NPs. Two million DCs were incubated with 500 μg hybrid NPs that underwent 30 days’ storage at 4 °C in PBS buffer for 120 min. The cellular uptake of the NPs which were labeled with Alexa 647 were quantitatively and qualitatively studied using a BD FACSARIA flow cytometer and an Amnis ® ImageStream flow cytometer, respectively. (A) Dots plot of cellular uptake of NPs. (B) The Alexa 647 median intensity in the DCs. (C) Representative microscopic images of cellular uptake of the hybrid NPs. *** in Fig. 4. A means the percentage of cells internalized NPs that contained more PEG was significantly higher than those with less PEG in the lipid layer. *** denotes a P-value that is less than 0.01.

    Article Snippet: Cell samples were immediately analyzed by a BD FACSARIA flow cytometer (BD, Franklin Lakes, NJ) and a Amnis® ImageStream® X Mark II flow cytometer (MilliporeSigma, Billerica, MA), respectively.

    Techniques: Incubation, Labeling, Flow Cytometry, Cytometry

    API5 expression is increased in cancer stem-like cell-enriched populations. ( a ) Flow cytometry analysis of CD44 expression in CaSki/D b P0 and P3 cells. The bar graph depicts the percentage of CD44 (mean±s.d.). ( b ) Western blot analysis of expression of API5, FGF2 and NANOG in CaSki/D b P0 and P3 cells. ( c ) CD44 high or CD44 low cells were sorted from CaSki/D b P3 cells by using FACSAria III flow cytometer (left panel). Flow cytometry analysis of CD44 expression in CD44 high or CD44 low cells (right panel). ( d ) Western blot analysis of expression of API5, FGF2 and NANOG in CD44 high or CD44 low cells. ( e ) Flow cytometry analysis of CD44 in CaSki cells grown in monolayer cultures (monolayers) and spheres isolated from CaSki cells under suspension conditions (spheres). The bar graph depicts the percentage of CD44 (mean±s.d.). ( f ) Western blot analysis of expression of API5, FGF2 and NANOG in spheres and monolayers of CaSki cells. ( b , d , f ) Numbers below blots indicate the expression as measured by fold change.

    Journal: Oncogenesis

    Article Title: API5 confers cancer stem cell-like properties through the FGF2-NANOG axis

    doi: 10.1038/oncsis.2016.87

    Figure Lengend Snippet: API5 expression is increased in cancer stem-like cell-enriched populations. ( a ) Flow cytometry analysis of CD44 expression in CaSki/D b P0 and P3 cells. The bar graph depicts the percentage of CD44 (mean±s.d.). ( b ) Western blot analysis of expression of API5, FGF2 and NANOG in CaSki/D b P0 and P3 cells. ( c ) CD44 high or CD44 low cells were sorted from CaSki/D b P3 cells by using FACSAria III flow cytometer (left panel). Flow cytometry analysis of CD44 expression in CD44 high or CD44 low cells (right panel). ( d ) Western blot analysis of expression of API5, FGF2 and NANOG in CD44 high or CD44 low cells. ( e ) Flow cytometry analysis of CD44 in CaSki cells grown in monolayer cultures (monolayers) and spheres isolated from CaSki cells under suspension conditions (spheres). The bar graph depicts the percentage of CD44 (mean±s.d.). ( f ) Western blot analysis of expression of API5, FGF2 and NANOG in spheres and monolayers of CaSki cells. ( b , d , f ) Numbers below blots indicate the expression as measured by fold change.

    Article Snippet: To isolate CD44high or CD44low cells, stained cells were washed twice, resuspended in PBS and sorted by using FACSAria III flow cytometer (BD Biosciences, San Jose, CA, USA).

    Techniques: Expressing, Flow Cytometry, Cytometry, Western Blot, Isolation