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  • 99
    Thermo Fisher facs buffer
    Facs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 11601 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore facs buffer
    Facs Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson facs buffer
    Impact of CD71 + erythroid cells on inflammatory-mediated mortality in neonates A. Survival after adoptive transfer with endotoxin exposure. Neonatal mice received 3×10 6 highly enriched neonatal CD71 + erythroid (Ter119 + ) cells via IP injection 30 minutes prior to endotoxin challenge (LD 40 ). B. Survival after adoptive transfer with polymicrobial sepsis. Neonatal mice received 5×10 6 highly enriched neonatal splenic CD71 + erythroid cells, <t>B220</t> + cells, or PBS via IP injection 30 minutes prior to sepsis (LD 60 ). C. Gross spleen appearance and splenic CD71 + erythroid cell representation by <t>FACS</t> in previously healthy neonatal mice following anti-CD71 or isotype antibody treatment. Mean with std dev shown. *-p
    Facs Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 30035 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson facs staining buffer
    Different γ-irradiation regimes induce different modalities of cell death and senescence in breast cancer cell lines. (A) Induction of apoptosis, necrosis, and senescence. Breast cancer cell lines were left untreated or γ-irradiated at single doses of 2 Gy, 20 Gy, or daily fractions of 2 Gy, respectively. Induction of apoptosis and necrosis was determined 1–4 days after irradiation by annexin <t>V-FITC/PI</t> staining and <t>FACS</t> analysis. Annexin V-FITC positive, PI negative cells were considered apoptotic, double positive cells were considered necrotic. Senescence induction was measured by flow cytometric SA-β-gal staining with the fluorogenic substrate C12-FDG-FITC. Cells with high C12-FDG-FITC and high SSC signal were considered senescent. Means ± s.d. of triplicates are depicted. (B) Induction of apoptosis and necrosis in the presence of zVAD-fmk. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the poly-caspase inhibitor zVAD-fmk. Means ± s.d. of triplicates are shown. (C) Induction of apoptosis and necrosis in the presence of necrostatin-1. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the necroptosis inhibitor necrostatin-1. Means ± s.d. of triplicates are shown. (D) Representative dot plots of HCC1937 cells stained for SA-β-gal activity with the fluorogenic substrate C12-FDG-FITC.
    Facs Staining Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 1424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson facs wash buffer
    Different γ-irradiation regimes induce different modalities of cell death and senescence in breast cancer cell lines. (A) Induction of apoptosis, necrosis, and senescence. Breast cancer cell lines were left untreated or γ-irradiated at single doses of 2 Gy, 20 Gy, or daily fractions of 2 Gy, respectively. Induction of apoptosis and necrosis was determined 1–4 days after irradiation by annexin <t>V-FITC/PI</t> staining and <t>FACS</t> analysis. Annexin V-FITC positive, PI negative cells were considered apoptotic, double positive cells were considered necrotic. Senescence induction was measured by flow cytometric SA-β-gal staining with the fluorogenic substrate C12-FDG-FITC. Cells with high C12-FDG-FITC and high SSC signal were considered senescent. Means ± s.d. of triplicates are depicted. (B) Induction of apoptosis and necrosis in the presence of zVAD-fmk. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the poly-caspase inhibitor zVAD-fmk. Means ± s.d. of triplicates are shown. (C) Induction of apoptosis and necrosis in the presence of necrostatin-1. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the necroptosis inhibitor necrostatin-1. Means ± s.d. of triplicates are shown. (D) Representative dot plots of HCC1937 cells stained for SA-β-gal activity with the fluorogenic substrate C12-FDG-FITC.
    Facs Wash Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 604 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson facs lysis buffer
    Effect fixative-containing <t>RBC</t> lysis buffer on cell yield. ( A ) Typical scatter plot of samples treated with fixative-containing lysis buffer. ( B ) Typical scatter plot of samples treated with fixative-free lysis buffer. ( C ) Effect of fixative in RBC lysis buffer on cell yield during <t>FACS</t> acquisition using counting beads compensation. ( D ) Effect of fixative in RBC lysis buffer on the percentage of lymphocyte and monocyte populations. Data are presented as geometric means with 95% confidence intervals.
    Facs Lysis Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Becton Dickinson facs lysing buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Lysing Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 357 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher fluorescence activated cell sorter staining buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Fluorescence Activated Cell Sorter Staining Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson facs stain buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Stain Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher facs wash buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Becton Dickinson facs flow buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Flow Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Becton Dickinson facs lyse buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Lyse Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Pharmingen facs buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Buffer, supplied by Pharmingen, used in various techniques. Bioz Stars score: 92/100, based on 607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson fluorescence activated cell sorting facs permeabilization buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Fluorescence Activated Cell Sorting Facs Permeabilization Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher fluorescence activated cell sorting facs buffer buffer pbs
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Fluorescence Activated Cell Sorting Facs Buffer Buffer Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Atlanta Biologicals facs buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Buffer, supplied by Atlanta Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cellgro facs buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Buffer, supplied by Cellgro, used in various techniques. Bioz Stars score: 92/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    facs buffer - by Bioz Stars, 2020-11
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    88
    Becton Dickinson facs perm buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Perm Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biochrom facs buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Buffer, supplied by Biochrom, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Corning Life Sciences facs buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Buffer, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    BioLegend facs buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Buffer, supplied by BioLegend, used in various techniques. Bioz Stars score: 96/100, based on 3727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Carl Roth GmbH facs buffer
    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + <t>/CD45</t> − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and <t>FACS</t> isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P
    Facs Buffer, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Impact of CD71 + erythroid cells on inflammatory-mediated mortality in neonates A. Survival after adoptive transfer with endotoxin exposure. Neonatal mice received 3×10 6 highly enriched neonatal CD71 + erythroid (Ter119 + ) cells via IP injection 30 minutes prior to endotoxin challenge (LD 40 ). B. Survival after adoptive transfer with polymicrobial sepsis. Neonatal mice received 5×10 6 highly enriched neonatal splenic CD71 + erythroid cells, B220 + cells, or PBS via IP injection 30 minutes prior to sepsis (LD 60 ). C. Gross spleen appearance and splenic CD71 + erythroid cell representation by FACS in previously healthy neonatal mice following anti-CD71 or isotype antibody treatment. Mean with std dev shown. *-p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Neonatal CD71+ erythroid cells do not modify murine sepsis mortality

    doi: 10.4049/jimmunol.1500771

    Figure Lengend Snippet: Impact of CD71 + erythroid cells on inflammatory-mediated mortality in neonates A. Survival after adoptive transfer with endotoxin exposure. Neonatal mice received 3×10 6 highly enriched neonatal CD71 + erythroid (Ter119 + ) cells via IP injection 30 minutes prior to endotoxin challenge (LD 40 ). B. Survival after adoptive transfer with polymicrobial sepsis. Neonatal mice received 5×10 6 highly enriched neonatal splenic CD71 + erythroid cells, B220 + cells, or PBS via IP injection 30 minutes prior to sepsis (LD 60 ). C. Gross spleen appearance and splenic CD71 + erythroid cell representation by FACS in previously healthy neonatal mice following anti-CD71 or isotype antibody treatment. Mean with std dev shown. *-p

    Article Snippet: Isolated or enriched murine splenic leukocytes were phenotyped by cell surface staining with B220, CD71, Ter119, 7-aminoactinomycin D (7-AAD, eBioscience, BD biosciences) in FACS buffer (PBS with 3% FBS with no azide) on a BD Fortessa.

    Techniques: Adoptive Transfer Assay, Mouse Assay, Injection, FACS

    CD71 + CD235a + cells are prominent in human neonatal cord blood and are predominantly enucleated reticulocytes A. Representative FACS plots for post-Ficoll mononuclear fraction of healthy adult peripheral blood, healthy term neonate cord blood, healthy term neonate cord blood after hypotonic lysis, enrichment of neonatal cord blood CD71 + 235a + cells, H E stain of enriched CD71 + CD235a + cells (top), methylene blue stain of enriched CD71 + CD235a + cells (bottom, inset showing appearance of nucleated cell). B. Effect of hypotonic lysis on CD71 + CD235a + cells from healthy adult peripheral blood and healthy neonatal cord blood after Ficoll-based isolation of mononuclear cells.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Neonatal CD71+ erythroid cells do not modify murine sepsis mortality

    doi: 10.4049/jimmunol.1500771

    Figure Lengend Snippet: CD71 + CD235a + cells are prominent in human neonatal cord blood and are predominantly enucleated reticulocytes A. Representative FACS plots for post-Ficoll mononuclear fraction of healthy adult peripheral blood, healthy term neonate cord blood, healthy term neonate cord blood after hypotonic lysis, enrichment of neonatal cord blood CD71 + 235a + cells, H E stain of enriched CD71 + CD235a + cells (top), methylene blue stain of enriched CD71 + CD235a + cells (bottom, inset showing appearance of nucleated cell). B. Effect of hypotonic lysis on CD71 + CD235a + cells from healthy adult peripheral blood and healthy neonatal cord blood after Ficoll-based isolation of mononuclear cells.

    Article Snippet: Isolated or enriched murine splenic leukocytes were phenotyped by cell surface staining with B220, CD71, Ter119, 7-aminoactinomycin D (7-AAD, eBioscience, BD biosciences) in FACS buffer (PBS with 3% FBS with no azide) on a BD Fortessa.

    Techniques: FACS, Lysis, Staining, Isolation

    Different γ-irradiation regimes induce different modalities of cell death and senescence in breast cancer cell lines. (A) Induction of apoptosis, necrosis, and senescence. Breast cancer cell lines were left untreated or γ-irradiated at single doses of 2 Gy, 20 Gy, or daily fractions of 2 Gy, respectively. Induction of apoptosis and necrosis was determined 1–4 days after irradiation by annexin V-FITC/PI staining and FACS analysis. Annexin V-FITC positive, PI negative cells were considered apoptotic, double positive cells were considered necrotic. Senescence induction was measured by flow cytometric SA-β-gal staining with the fluorogenic substrate C12-FDG-FITC. Cells with high C12-FDG-FITC and high SSC signal were considered senescent. Means ± s.d. of triplicates are depicted. (B) Induction of apoptosis and necrosis in the presence of zVAD-fmk. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the poly-caspase inhibitor zVAD-fmk. Means ± s.d. of triplicates are shown. (C) Induction of apoptosis and necrosis in the presence of necrostatin-1. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the necroptosis inhibitor necrostatin-1. Means ± s.d. of triplicates are shown. (D) Representative dot plots of HCC1937 cells stained for SA-β-gal activity with the fluorogenic substrate C12-FDG-FITC.

    Journal: Radiation Oncology (London, England)

    Article Title: Release of monocyte migration signals by breast cancer cell lines after ablative and fractionated ?-irradiation

    doi: 10.1186/1748-717X-9-85

    Figure Lengend Snippet: Different γ-irradiation regimes induce different modalities of cell death and senescence in breast cancer cell lines. (A) Induction of apoptosis, necrosis, and senescence. Breast cancer cell lines were left untreated or γ-irradiated at single doses of 2 Gy, 20 Gy, or daily fractions of 2 Gy, respectively. Induction of apoptosis and necrosis was determined 1–4 days after irradiation by annexin V-FITC/PI staining and FACS analysis. Annexin V-FITC positive, PI negative cells were considered apoptotic, double positive cells were considered necrotic. Senescence induction was measured by flow cytometric SA-β-gal staining with the fluorogenic substrate C12-FDG-FITC. Cells with high C12-FDG-FITC and high SSC signal were considered senescent. Means ± s.d. of triplicates are depicted. (B) Induction of apoptosis and necrosis in the presence of zVAD-fmk. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the poly-caspase inhibitor zVAD-fmk. Means ± s.d. of triplicates are shown. (C) Induction of apoptosis and necrosis in the presence of necrostatin-1. PS externalization and plasma membrane integrity were measured as in (A) in the presence of 50 μM of the necroptosis inhibitor necrostatin-1. Means ± s.d. of triplicates are shown. (D) Representative dot plots of HCC1937 cells stained for SA-β-gal activity with the fluorogenic substrate C12-FDG-FITC.

    Article Snippet: For ectonucleotidase surface staining, 1 × 105 cells were incubated with 2 μl anti-CD39-PE, anti-CD73-FITC, or anti-CD203c-APC in 50 μl FACS staining buffer (all from BD Biosciences) for 30 min on ice.

    Techniques: Irradiation, Staining, FACS, Flow Cytometry, Activity Assay

    Effect fixative-containing RBC lysis buffer on cell yield. ( A ) Typical scatter plot of samples treated with fixative-containing lysis buffer. ( B ) Typical scatter plot of samples treated with fixative-free lysis buffer. ( C ) Effect of fixative in RBC lysis buffer on cell yield during FACS acquisition using counting beads compensation. ( D ) Effect of fixative in RBC lysis buffer on the percentage of lymphocyte and monocyte populations. Data are presented as geometric means with 95% confidence intervals.

    Journal: PLoS ONE

    Article Title: Stepwise Optimization of the Procedure for Assessment of Circulating Progenitor Cells in Patients with Myocardial Infarction

    doi: 10.1371/journal.pone.0030389

    Figure Lengend Snippet: Effect fixative-containing RBC lysis buffer on cell yield. ( A ) Typical scatter plot of samples treated with fixative-containing lysis buffer. ( B ) Typical scatter plot of samples treated with fixative-free lysis buffer. ( C ) Effect of fixative in RBC lysis buffer on cell yield during FACS acquisition using counting beads compensation. ( D ) Effect of fixative in RBC lysis buffer on the percentage of lymphocyte and monocyte populations. Data are presented as geometric means with 95% confidence intervals.

    Article Snippet: For the lyse/wash procedure, samples are subjected to lysis of red blood cells (RBC) for 15 min at room temperature using either 1× FACS lysis buffer (BD Bioscience, San Jose, CA) or high yield fixative free lysis buffer (Invitrogen, Paisley, UK).

    Techniques: Lysis, FACS

    Comparison of lyse-wash and lyse-no-wash procedures. Whole blood samples were stained with progenitor cell markers and eFluor 780 fixable viability dye. Following RBC lysis with FCS lysis buffer (BD Bioscience), stained samples were washed with PBS ( A , C and E ) or stored at 4°C for subsequent FACS analysis within 5 days ( B , D and F ).

    Journal: PLoS ONE

    Article Title: Stepwise Optimization of the Procedure for Assessment of Circulating Progenitor Cells in Patients with Myocardial Infarction

    doi: 10.1371/journal.pone.0030389

    Figure Lengend Snippet: Comparison of lyse-wash and lyse-no-wash procedures. Whole blood samples were stained with progenitor cell markers and eFluor 780 fixable viability dye. Following RBC lysis with FCS lysis buffer (BD Bioscience), stained samples were washed with PBS ( A , C and E ) or stored at 4°C for subsequent FACS analysis within 5 days ( B , D and F ).

    Article Snippet: For the lyse/wash procedure, samples are subjected to lysis of red blood cells (RBC) for 15 min at room temperature using either 1× FACS lysis buffer (BD Bioscience, San Jose, CA) or high yield fixative free lysis buffer (Invitrogen, Paisley, UK).

    Techniques: Staining, Lysis, FACS

    Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + /CD45 − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and FACS isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Myeloid-epithelial cross talk coordinates synthesis of the tissue-protective cytokine leukemia inhibitory factor during pneumonia

    doi: 10.1152/ajplung.00482.2016

    Figure Lengend Snippet: Production of LIF RNA lung epithelial cells. A : SPC-GFP mice were infected with E. coli or saline control for 6 h. Left lobes were digested to single-cell suspension with elastase. Epithelial cells (CD326 + /CD45 − ) were subtyped into ATI (GFP − /T1α + /CD24 mid ), ATII (GFP + ), and ciliated cells (GFP − /T1α − /CD24 hi ) and FACS isolated. RNA was isolated from sorted cells, and Pdpn1 ( B ), SPC ( C ), and FoxJ1 ( D ) were measured in saline-treated controls. Values represent fold change compared with uninfected whole lung control, n = 3–6. * P

    Article Snippet: For peripheral blood, heparinized blood was collected from the inferior vena cava, and red blood cells were lysed with FACS lysing buffer (BD Biosciences) before neutrophils (7AAD− /CD45+ /CD11b+ /Ly6G+ ) and monocytes (7AAD− /CD45+ /CD11b+ /CD115+ ) were isolated.

    Techniques: Mouse Assay, Infection, FACS, Isolation

    Production of LIF RNA in various lung cell types. Left : representative flow plots of FACS strategies. Right : LIF RNA production in each cell type. Values represent fold change compared with uninfected whole lung control, n = 4–6. A : left lobes were digested to single-cell suspension with elastase, and epithelial cells (7AAD − /CD326 + /CD45 − ), neutrophils (7AAD − /CD45 + /Ly6G + /F4-80 − ), and macrophages (7AAD − /CD45 + /Ly6G − /F4-80 + ) were FACS isolated. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Myeloid-epithelial cross talk coordinates synthesis of the tissue-protective cytokine leukemia inhibitory factor during pneumonia

    doi: 10.1152/ajplung.00482.2016

    Figure Lengend Snippet: Production of LIF RNA in various lung cell types. Left : representative flow plots of FACS strategies. Right : LIF RNA production in each cell type. Values represent fold change compared with uninfected whole lung control, n = 4–6. A : left lobes were digested to single-cell suspension with elastase, and epithelial cells (7AAD − /CD326 + /CD45 − ), neutrophils (7AAD − /CD45 + /Ly6G + /F4-80 − ), and macrophages (7AAD − /CD45 + /Ly6G − /F4-80 + ) were FACS isolated. * P

    Article Snippet: For peripheral blood, heparinized blood was collected from the inferior vena cava, and red blood cells were lysed with FACS lysing buffer (BD Biosciences) before neutrophils (7AAD− /CD45+ /CD11b+ /Ly6G+ ) and monocytes (7AAD− /CD45+ /CD11b+ /CD115+ ) were isolated.

    Techniques: Flow Cytometry, FACS, Isolation