Journal: Biology of reproduction

Article Title: Immunolocalization of acidic and basic fibroblast growth factors in porcine uterine and conceptus tissues.

doi: 10.1095/biolreprod56.6.1527

Figure Lengend Snippet: FIG. 1. Western blot analysis of porcine uterine protein extract at Day 12 of gestation. a) Molecular weight markers (x 10 3) (lane 1), total CM-50 sephadex-extracted uterine protein (25 g, lanes 2, 4, and 6), human recombinant bFGF (100 ng, lanes 3 and 5). Lanes 1 and 2 were stained for total protein by colloidal gold stain. Lanes 3 and 4 were in- cubated with anti-bFGF antibody (0.15 Ixg/ml). Lanes 5 and 6 were in- cubated with rabbit IgG (0.15 .Lg/ml). b) Sephadex-extracted uterine pro- tein (25 jIg, stained for total protein by colloidal gold) in nonreducing 7.5-15% PAGE. Bovine aFGF (500 ng, lanes 2, 4, and 6) and sephadex extract (25 g, lanes 3, 5, and 7). Immunoreactive aFGF bands are ob- served in lanes 2 and 3 (incubated with anti-aFGF antibody). The absence of these bands is noted in lanes 4 and 5 (anti-aFGF antibody immunoab- sorbed with bovine aFGF) and in lanes 6 and 7 (incubated with rabbit IgG).

Article Snippet: For aFGE PAGE was performed using 7.5-15% acrylamide gel and bovine aFGF (cat. #132-FA; R&D Systems, Minneapolis, MN) as standard.

Techniques: Western Blot, Molecular Weight, Recombinant, Staining, Incubation