Journal: Frontiers in Immunology

Article Title: The Nrf2-SLPI axis in aging and its role in the pathophysiology of pulmonary Mycobacterium avium complex disease

doi: 10.3389/fimmu.2026.1733057

Figure Lengend Snippet: Localization of SLPI. (A) Validation of RNA-seq data by performing RT-qPCR for SLPI in lung tissues 2 months after MAC infection. Experiments were performed in duplicate with three mice per group. (B) Representative photographs of immunostaining for SLPI in the lungs of young and old mice 2 months after MAC infection (×40 and ×200). (C) Representative images showing SLPI expression in the lungs of young and old mice 2 months after MAC infection (immunofluorescence, SLPI in green, F4/80 in red, and DAPI in blue) (×200). Significant difference between each group (p < 0.05). Data are expressed as mean ± SEM values.

Article Snippet: For immunofluorescence staining, lung sections were incubated with appropriately diluted primary antibodies (SLPI [NOVUS: NBP1-76803] and F4/80 [Cell Signaling TECHNOLOGY: #70076S]).

Techniques: Biomarker Discovery, RNA Sequencing, Quantitative RT-PCR, Infection, Immunostaining, Expressing, Immunofluorescence