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  • 99
    Thermo Fisher extraction buffer
    OAP-ELISA development workflow. Plasma membrane proteins were extracted under native conditions from rat brain plasma membrane <t>vescicles</t> and subjected to <t>nSEC</t> to isolate AQP4-OAPs. Native AQP4-OAPs were then immobilized on a plastic plate using a commercial AQP4 antibody with the sandwich approach. Indirect anti-human-biotin/streptavidin-HRP based AQP4-IgG detection was performed. Note that the commercial antibody recognizes the intracellular region of AQP4, while AQP4-IgG autoantibodies recognize the extracellular regions. Thus, the C-terminal anti-AQP4 antibody allows the correct orientation of AQP4-OAPs for AQP4-IgG binding.
    Extraction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4868 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extraction buffer/product/Thermo Fisher
    Average 99 stars, based on 4868 article reviews
    Price from $9.99 to $1999.99
    extraction buffer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    92
    Millipore extraction buffer
    OAP-ELISA development workflow. Plasma membrane proteins were extracted under native conditions from rat brain plasma membrane <t>vescicles</t> and subjected to <t>nSEC</t> to isolate AQP4-OAPs. Native AQP4-OAPs were then immobilized on a plastic plate using a commercial AQP4 antibody with the sandwich approach. Indirect anti-human-biotin/streptavidin-HRP based AQP4-IgG detection was performed. Note that the commercial antibody recognizes the intracellular region of AQP4, while AQP4-IgG autoantibodies recognize the extracellular regions. Thus, the C-terminal anti-AQP4 antibody allows the correct orientation of AQP4-OAPs for AQP4-IgG binding.
    Extraction Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 6906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extraction buffer/product/Millipore
    Average 92 stars, based on 6906 article reviews
    Price from $9.99 to $1999.99
    extraction buffer - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    94
    Abcam extraction buffer
    OAP-ELISA development workflow. Plasma membrane proteins were extracted under native conditions from rat brain plasma membrane <t>vescicles</t> and subjected to <t>nSEC</t> to isolate AQP4-OAPs. Native AQP4-OAPs were then immobilized on a plastic plate using a commercial AQP4 antibody with the sandwich approach. Indirect anti-human-biotin/streptavidin-HRP based AQP4-IgG detection was performed. Note that the commercial antibody recognizes the intracellular region of AQP4, while AQP4-IgG autoantibodies recognize the extracellular regions. Thus, the C-terminal anti-AQP4 antibody allows the correct orientation of AQP4-OAPs for AQP4-IgG binding.
    Extraction Buffer, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/extraction buffer/product/Abcam
    Average 94 stars, based on 123 article reviews
    Price from $9.99 to $1999.99
    extraction buffer - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    99
    Millipore sigma extraction buffer
    OAP-ELISA development workflow. Plasma membrane proteins were extracted under native conditions from rat brain plasma membrane <t>vescicles</t> and subjected to <t>nSEC</t> to isolate AQP4-OAPs. Native AQP4-OAPs were then immobilized on a plastic plate using a commercial AQP4 antibody with the sandwich approach. Indirect anti-human-biotin/streptavidin-HRP based AQP4-IgG detection was performed. Note that the commercial antibody recognizes the intracellular region of AQP4, while AQP4-IgG autoantibodies recognize the extracellular regions. Thus, the C-terminal anti-AQP4 antibody allows the correct orientation of AQP4-OAPs for AQP4-IgG binding.
    Sigma Extraction Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sigma extraction buffer/product/Millipore
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    sigma extraction buffer - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    OAP-ELISA development workflow. Plasma membrane proteins were extracted under native conditions from rat brain plasma membrane vescicles and subjected to nSEC to isolate AQP4-OAPs. Native AQP4-OAPs were then immobilized on a plastic plate using a commercial AQP4 antibody with the sandwich approach. Indirect anti-human-biotin/streptavidin-HRP based AQP4-IgG detection was performed. Note that the commercial antibody recognizes the intracellular region of AQP4, while AQP4-IgG autoantibodies recognize the extracellular regions. Thus, the C-terminal anti-AQP4 antibody allows the correct orientation of AQP4-OAPs for AQP4-IgG binding.

    Journal: PLoS ONE

    Article Title: Development of an Aquaporin-4 Orthogonal Array of Particle-Based ELISA for Neuromyelitis Optica Autoantibodies Detection

    doi: 10.1371/journal.pone.0143679

    Figure Lengend Snippet: OAP-ELISA development workflow. Plasma membrane proteins were extracted under native conditions from rat brain plasma membrane vescicles and subjected to nSEC to isolate AQP4-OAPs. Native AQP4-OAPs were then immobilized on a plastic plate using a commercial AQP4 antibody with the sandwich approach. Indirect anti-human-biotin/streptavidin-HRP based AQP4-IgG detection was performed. Note that the commercial antibody recognizes the intracellular region of AQP4, while AQP4-IgG autoantibodies recognize the extracellular regions. Thus, the C-terminal anti-AQP4 antibody allows the correct orientation of AQP4-OAPs for AQP4-IgG binding.

    Article Snippet: Native Size Exclusion Chromatography (nSEC) For nSEC analysis, rat brain plasma membrane vescicles were extracted in nSEC-Extraction buffer (Extraction buffer added with 150mM NaCl) and the protein content was measured by BCA protein assay (Thermo, www.thermofisher.com ).

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    Extraction evaluation for maintaining OAP integrity and AQP4-IgG binding. (A) AQP4 immunoblotting after BN-PAGE of rat brain membrane vescicles extracted in native conditions using five different 3% DDM containing buffers. Buffers containing 0%, 2% or 10% of glycerol in combination with 12 or 150 mM NaCl were tested. Note that all tested extraction conditions were able to preserve OAP structure. (B) Left, AQP4 immunoblotting after immunoprecipitation with commercial AQP4 antibody, three NMO and one MS sera, using BN-Bufferand nSEC compatible buffer (arrowhead in A). High AQP4-IgG titer (NMO1 and 2), and low titer (NMO3) were used. Right, densitometric analysis showing the commercial anti-AQP4 normalized AQP4 immunoprecipitated by AQP4-IgG (n = 3, *p

    Journal: PLoS ONE

    Article Title: Development of an Aquaporin-4 Orthogonal Array of Particle-Based ELISA for Neuromyelitis Optica Autoantibodies Detection

    doi: 10.1371/journal.pone.0143679

    Figure Lengend Snippet: Extraction evaluation for maintaining OAP integrity and AQP4-IgG binding. (A) AQP4 immunoblotting after BN-PAGE of rat brain membrane vescicles extracted in native conditions using five different 3% DDM containing buffers. Buffers containing 0%, 2% or 10% of glycerol in combination with 12 or 150 mM NaCl were tested. Note that all tested extraction conditions were able to preserve OAP structure. (B) Left, AQP4 immunoblotting after immunoprecipitation with commercial AQP4 antibody, three NMO and one MS sera, using BN-Bufferand nSEC compatible buffer (arrowhead in A). High AQP4-IgG titer (NMO1 and 2), and low titer (NMO3) were used. Right, densitometric analysis showing the commercial anti-AQP4 normalized AQP4 immunoprecipitated by AQP4-IgG (n = 3, *p

    Article Snippet: Native Size Exclusion Chromatography (nSEC) For nSEC analysis, rat brain plasma membrane vescicles were extracted in nSEC-Extraction buffer (Extraction buffer added with 150mM NaCl) and the protein content was measured by BCA protein assay (Thermo, www.thermofisher.com ).

    Techniques: Binding Assay, Polyacrylamide Gel Electrophoresis, Immunoprecipitation, Mass Spectrometry