Journal: bioRxiv

Article Title: The proteomic and transcriptomic landscapes altered by Rgg2/3 activity in Streptococcus pyogenes

doi: 10.1101/2022.05.06.490990

Figure Lengend Snippet: qRT-PCR results verifying RNA-seq and examining if proteomics targets have altered transcript levels. Wild-type NZ131, Δ rgg2 , and Δ rgg3 strains were grown in biological triplicate with or without 100 nM SHP peptide (indicated by + or – sign below each graph) and RNA was harvested late exponential phase. RNA was processed for qRT-PCR and transcript levels were determine relative to the gyrA reference gene. Significance of transcript level changes were determined using a One-way ANOVA with Multiple Comparisons Post-test. *, P < 0.05; **, P < 0.005; ****, P < 0.0005; ns, non-significant. For further experimental details, see Materials and Methods . A) Relative transcript levels of spy49_0450 (aroE.2) . B) Relative transcript levels of stcA ( spy49_0414c ). C) Relative transcript levels of slo ( spy49_0146 ). D) Relative transcript levels of sfbX49 ( spy49_1683c ). E) Relative transcript levels of upp ( spy49_0322 ). F) Relative transcript levels of spy49_0877 .

Article Snippet: Data was plotted and doubling times were calculated by taking OD 600 s ∼0.015 – 0.9 and performing a Nonlinear regression analysis with Exponential growth equation fitting with Graph Pad Prism 9.2.0 (GraphPad Software).

Techniques: Quantitative RT-PCR, RNA Sequencing

Journal: Membranes

Article Title: An Assessment on Average Pressure Drop and Dust-Holding Capacity of Hollow-Fiber Membranes in Air Filtration

doi: 10.3390/membranes11070467

Figure Lengend Snippet: Dust loading curves fitted with different models: ( a ) various membranes and velocities at a constant loading rate of 4 g/h with exponential fit (the minor x -axis in red is for P60, at 40 cm/s), ( b ) the same fitted with 4th order polynomial (the minor x -axis in red is for P60, at 40 cm/s), ( c ) P80 membrane at 20 cm/s at different loading rates with exponential fit, ( d ) the same fitted with 4th order polynomial.

Article Snippet: The data obtained for this membrane was thus possible to approximate using the exponential curve fit, which is generally done in the ASHRAE dust-holding capacity test [ ] and which was previously used in several studies [ , ].

Techniques: Membrane

Journal: Membranes

Article Title: An Assessment on Average Pressure Drop and Dust-Holding Capacity of Hollow-Fiber Membranes in Air Filtration

doi: 10.3390/membranes11070467

Figure Lengend Snippet: Dust-holding capacity per cake pressure drop of different membranes at different permeate velocities at a constant loading rate of 4 g/h ( a ) and P80 membrane at 20 cm/s at different loading rates ( b ), DHC measured ( DHC meas ), DHC from exponential fit ( DHC exp ) and DHC from polynomial fit ( DHC pol ) are at the major axis, DHC theoretical ( DHC theor ) is at the minor axis.

Article Snippet: The data obtained for this membrane was thus possible to approximate using the exponential curve fit, which is generally done in the ASHRAE dust-holding capacity test [ ] and which was previously used in several studies [ , ].

Techniques: Membrane