Journal: Annals of Translational Medicine

Article Title: AZD9291-resistant non-small cell lung cancer cell-derived exosomal lnc-MZT2A-5:1 induces the activation of fibroblasts

doi: 10.21037/atm-21-5186

Figure Lengend Snippet: Characterization of exosomes derived from wild type or AZD9291-resistant H1975/PC9 cells. (A) NTA analysis of the size distribution, and TNM images of exosomes shown by the red arrows. (B) Western blotting analysis for exosomal markers (CD81, CD63, Hsp27, and TSG101) of exosomes. NTA, nanoparticle tracking analysis.

Article Snippet: Exosomal lncRNAs microarray analysis was performed at the Shanghai Biotechnology Corporation (Shanghai, China), using Agilent Human miRNA 8*60K V21.0 microarray (Agilent Technologies, USA).

Techniques: Derivative Assay, Western Blot

Journal: Annals of Translational Medicine

Article Title: AZD9291-resistant non-small cell lung cancer cell-derived exosomal lnc-MZT2A-5:1 induces the activation of fibroblasts

doi: 10.21037/atm-21-5186

Figure Lengend Snippet: Exosomal lnc-MZT2A-5:1 promoted the migration and inflammation of MRC-5 cells. (A) Scatter plot of differentially expressed lncRNAs in exosomes derived from wild type and AZD9291-resistant NSCLC cells stained by crystal violet (red dot: significantly increased; blue dot: significantly decreased). (B) The expression levels of five candidate lncRNAs in exosomes derived from wild type or AZD9291-resistant NSCLC cells. (C) Representative images and statistical analysis of transwell assays analysis of MRC-5 cells overexpressed with or without lnc-MZT2A-5:1 or lnc-CCDC103-7:1 (n=3), stained by crystal violet. (D) The mRNA levels of IL-1β, IL-6, and IL-8 in MRC-5 cells overexpressed with or without lnc-MZT2A-5:1 (n=3). A length of 50 µm was shown by scale bar and statistical significance was distinguished with P value: *, P<0.05; **, P<0.01; ***, P<0.001. NSCLC, non-small cell lung cancer.

Article Snippet: Exosomal lncRNAs microarray analysis was performed at the Shanghai Biotechnology Corporation (Shanghai, China), using Agilent Human miRNA 8*60K V21.0 microarray (Agilent Technologies, USA).

Techniques: Migration, Derivative Assay, Staining, Expressing

Journal: Annals of Translational Medicine

Article Title: AZD9291-resistant non-small cell lung cancer cell-derived exosomal lnc-MZT2A-5:1 induces the activation of fibroblasts

doi: 10.21037/atm-21-5186

Figure Lengend Snippet: Top 30 dysregulated exosomal lncRNAs in resistant cells

Article Snippet: Exosomal lncRNAs microarray analysis was performed at the Shanghai Biotechnology Corporation (Shanghai, China), using Agilent Human miRNA 8*60K V21.0 microarray (Agilent Technologies, USA).

Techniques:

Journal: Annals of Translational Medicine

Article Title: AZD9291-resistant non-small cell lung cancer cell-derived exosomal lnc-MZT2A-5:1 induces the activation of fibroblasts

doi: 10.21037/atm-21-5186

Figure Lengend Snippet: 15 candidate lncRNAs

Article Snippet: Exosomal lncRNAs microarray analysis was performed at the Shanghai Biotechnology Corporation (Shanghai, China), using Agilent Human miRNA 8*60K V21.0 microarray (Agilent Technologies, USA).

Techniques:

Journal: Frontiers in Aging Neuroscience

Article Title: Emerging Potential of Exosomal Non-coding RNA in Parkinson’s Disease: A Review

doi: 10.3389/fnagi.2022.819836

Figure Lengend Snippet: The role of exosomal ncRNA in neuroprotection, and pathogenesis, diagnosis, and treatment of PD.

Article Snippet: A study on Peripheral blood exosomal lncRNA expression levels in 32 PD patients and 13 healthy volunteers from Beijing Tiantan Hospital found 15 upregulated and 24 downregulated exosomal lncRNAs ( ).

Techniques: Biomarker Discovery

Journal: Frontiers in Aging Neuroscience

Article Title: Emerging Potential of Exosomal Non-coding RNA in Parkinson’s Disease: A Review

doi: 10.3389/fnagi.2022.819836

Figure Lengend Snippet: Overview of exosomal ncRNA in several PD related pathogenesis.

Article Snippet: A study on Peripheral blood exosomal lncRNA expression levels in 32 PD patients and 13 healthy volunteers from Beijing Tiantan Hospital found 15 upregulated and 24 downregulated exosomal lncRNAs ( ).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus

doi: 10.3389/fendo.2022.822221

Figure Lengend Snippet: Primer list. F- refers to the forward sequence, R- refers to the reverse sequence, and P- refers to the probe sequence.

Article Snippet: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Techniques: Sequencing

Journal: Frontiers in Endocrinology

Article Title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus

doi: 10.3389/fendo.2022.822221

Figure Lengend Snippet: The procedures of exosomal lncRNA sequencing and bioinformatics analysis.

Article Snippet: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Techniques: Sequencing

Journal: Frontiers in Endocrinology

Article Title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus

doi: 10.3389/fendo.2022.822221

Figure Lengend Snippet: Plasma-derived exosomal lncRNA profiles of case and control group. A heatmap (A) , volcano diagram (B) , and MA plot (C) of the 162 differentially expressed exosomal lncRNAs in T1DM and control subjects. G1: control group; G2: case group.

Article Snippet: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Techniques: Clinical Proteomics, Derivative Assay, Control

Journal: Frontiers in Endocrinology

Article Title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus

doi: 10.3389/fendo.2022.822221

Figure Lengend Snippet: The detailed information of the differentially expressed exosomal lncRNAs.

Article Snippet: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus

doi: 10.3389/fendo.2022.822221

Figure Lengend Snippet: GO terms of cis-acting genes of differentially expressed exosomal lncRNAs. GO categories of differentially expressed lncRNAs (A) . Top 20 significant enriched biological processes (B) , cellular components (C) , and molecular functions (D) of differentially expressed lncRNAs.

Article Snippet: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus

doi: 10.3389/fendo.2022.822221

Figure Lengend Snippet: GO terms of trans-acting genes of differentially expressed exosomal lncRNAs. GO categories of differentially expressed lncRNAs (A) . Top 20 significant enriched biological processes (B) , cellular components (C) , and molecular functions (D) of differentially expressed lncRNAs.

Article Snippet: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Techniques:

Journal: Frontiers in Endocrinology

Article Title: Characterization of lncRNA Profiles of Plasma-Derived Exosomes From Type 1 Diabetes Mellitus

doi: 10.3389/fendo.2022.822221

Figure Lengend Snippet: The qRT-PCR analysis of selected six exosomal lncRNAs in T1DM and control subjects (T1DM subjects N=30; age-, sex- matched Control subjects N=30). G1: control group; G2: case group.

Article Snippet: Exosomal lncRNA expression profiles were detected by Illumina Hiseq platform (T1DM subjects N=10; age-, sex- matched Control subjects N=10).

Techniques: Quantitative RT-PCR, Control