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Image Search Results
Journal: Endocrinology
Article Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues
doi: 10.1210/en.2016-1607
Figure Lengend Snippet: List of Antibodies
Article Snippet: Eva1 , Eva1 antibody ,
Techniques:
Journal: Endocrinology
Article Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues
doi: 10.1210/en.2016-1607
Figure Lengend Snippet: Primer Sequences Used for Quantitative Real-Time PCR
Article Snippet: After the treatment with enhanced chemiluminescence reagent (Pierce; Thermo Scientific), the membranes were exposed to an X-ray film (Kodak; Sigma Aldrich, St. Louis, MO) and Image by Image Quant (GE Lifescience, Pittsburgh, PA) software for densitometric analysis ( 28, 32 ). table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Peptide/Protein Target Name of Antibody Manufacturer, Catalog Number Antibody RRID Species Dilution Used UCP1 Uncoupling protein1 antibody Abcam ab10983 AB_2241462 Rabbit polyclonal 1:1000 UCP2 Uncoupling protein2 antibody Novus Biologicals NB100-78377 AB_1085929 Rabbit polyclonal 1:1000 UCP3 Uncoupling protein3 antibody Abcam ab3477 AB_2304253 Rabbit polyclonal 1:1000 PRDM16 PRDM16 antibody (N16) Santa Cruz Biotech sc-130243 AB_2284344 Rabbit polyclonal 1:500 PGC-1 α PGC-1 α antibody (H-300) Santa Cruz Biotech sc-13067 AB_2166218 Rabbit polyclonal 1:1000 Adiponectin Adiponectin antibody R & D Systems MAB1119 AB_2305045 Rat monoclonal 1:1000 AMPK α AMPK α antibody Cell Signaling 2532 AB_330331 Rabbit monoclonal 1:1000 pAMPK α pAMPK α antibody Cell Signaling 2535 AB_331250 Rabbit monoclonal 1:1000 Glut4 Glucose transporter 4 antibody Abcam ab65267 AB_1140009 Mouse monoclonal 1:1000 p38MAPK p38MAPK antibody Cell Signaling 9212S AB_330713 Rabbit polyclonal 1:1000 pp38MAPK pp38MAPK antibody Cell Signaling 9211S AB_331641 Rabbit polyclonal 1:1000 ERK1/2 ERK1/2 antibody Cell Signaling 9102S AB_330744 Rabbit polyclonal 1:1000 pERK1/2 pERK1/2 antibody Cell Signaling 9101S AB_331646 Rabbit polyclonal 1:1000 Myf5 Myf5 antibody Santa Cruz Biotech sc-302 AB_631994 Rabbit polyclonal 1:1000 Cd137 Cd137 antibody Abcam ab3169 AB_303572 Mouse monoclonal 1:1000 BMP7 BMP7 antibody Abcam ab54904 AB_940598 Mouse monoclonal 1:1000
Techniques:
Journal: Endocrinology
Article Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues
doi: 10.1210/en.2016-1607
Figure Lengend Snippet: List of Antibodies
Article Snippet: After the treatment with enhanced chemiluminescence reagent (Pierce; Thermo Scientific), the membranes were exposed to an X-ray film (Kodak; Sigma Aldrich, St. Louis, MO) and Image by Image Quant (GE Lifescience, Pittsburgh, PA) software for densitometric analysis ( 28, 32 ). table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Peptide/Protein Target Name of Antibody Manufacturer, Catalog Number Antibody RRID Species Dilution Used UCP1 Uncoupling protein1 antibody Abcam ab10983 AB_2241462 Rabbit polyclonal 1:1000 UCP2 Uncoupling protein2 antibody Novus Biologicals NB100-78377 AB_1085929 Rabbit polyclonal 1:1000 UCP3 Uncoupling protein3 antibody Abcam ab3477 AB_2304253 Rabbit polyclonal 1:1000 PRDM16 PRDM16 antibody (N16) Santa Cruz Biotech sc-130243 AB_2284344 Rabbit polyclonal 1:500 PGC-1 α PGC-1 α antibody (H-300) Santa Cruz Biotech sc-13067 AB_2166218 Rabbit polyclonal 1:1000 Adiponectin Adiponectin antibody R & D Systems MAB1119 AB_2305045 Rat monoclonal 1:1000 AMPK α AMPK α antibody Cell Signaling 2532 AB_330331 Rabbit monoclonal 1:1000 pAMPK α pAMPK α antibody Cell Signaling 2535 AB_331250 Rabbit monoclonal 1:1000 Glut4 Glucose transporter 4 antibody Abcam ab65267 AB_1140009 Mouse monoclonal 1:1000 p38MAPK p38MAPK antibody Cell Signaling 9212S AB_330713 Rabbit polyclonal 1:1000 pp38MAPK pp38MAPK antibody Cell Signaling 9211S AB_331641 Rabbit polyclonal 1:1000 ERK1/2 ERK1/2 antibody Cell Signaling 9102S AB_330744 Rabbit polyclonal 1:1000 pERK1/2 pERK1/2 antibody Cell Signaling 9101S AB_331646 Rabbit polyclonal 1:1000 Myf5 Myf5 antibody Santa Cruz Biotech sc-302 AB_631994 Rabbit polyclonal 1:1000 Cd137 Cd137 antibody Abcam ab3169 AB_303572 Mouse monoclonal 1:1000 BMP7 BMP7 antibody Abcam ab54904 AB_940598 Mouse monoclonal 1:1000
Techniques:
Journal: Endocrinology
Article Title: Follistatin Targets Distinct Pathways To Promote Brown Adipocyte Characteristics in Brown and White Adipose Tissues
doi: 10.1210/en.2016-1607
Figure Lengend Snippet: Upregulation of beige/brown adipose specific markers in 3T3-L1 and BAT cells after follistatin treatment. (a) 3T3-L1 cells were allowed to differentiate under modified adipogenic differentiation condition for 6 days either in absence or presence of recombinant Fst protein (0.5 µg/mL) as described in materials and methods. Protein expression of CD137 and UCP1 was analyzed by western blot analysis and a representative data are shown. (b) Densitometric analysis showing relative increase in UCP1 and CD137 protein levels (n = 3). **P ≤ 0.01. (c) Mouse BAT cells were allowed to differentiate under adipogenic differentiation conditions 6 days either in absence or presence of recombinant Fst protein (0.5 µg/mL) as described in materials and methods. Protein expression of UCP1, Eva1, and Myf5 was analyzed by western blot analysis and a representative data are shown. (d) Densitometric analysis showing relative increase in UCP1, Eva1, and Myf5 protein levels following Fst treatment (n = 3). *P ≤ 0.05; **P ≤ 0.01. (e) Quantitative gene expression analysis of key beige markers in differentiating 3T3-L1 cells treated with or without recombinant Fst (n = 3). *P ≤ 0.05; **P ≤ 0.01. (f) Quantitative gene expression analysis of key BAT markers in differentiating BAT cells treated with or without recombinant Fst (n = 3). *P ≤ 0.05; **P ≤ 0.01.
Article Snippet: After the treatment with enhanced chemiluminescence reagent (Pierce; Thermo Scientific), the membranes were exposed to an X-ray film (Kodak; Sigma Aldrich, St. Louis, MO) and Image by Image Quant (GE Lifescience, Pittsburgh, PA) software for densitometric analysis ( 28, 32 ). table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Peptide/Protein Target Name of Antibody Manufacturer, Catalog Number Antibody RRID Species Dilution Used UCP1 Uncoupling protein1 antibody Abcam ab10983 AB_2241462 Rabbit polyclonal 1:1000 UCP2 Uncoupling protein2 antibody Novus Biologicals NB100-78377 AB_1085929 Rabbit polyclonal 1:1000 UCP3 Uncoupling protein3 antibody Abcam ab3477 AB_2304253 Rabbit polyclonal 1:1000 PRDM16 PRDM16 antibody (N16) Santa Cruz Biotech sc-130243 AB_2284344 Rabbit polyclonal 1:500 PGC-1 α PGC-1 α antibody (H-300) Santa Cruz Biotech sc-13067 AB_2166218 Rabbit polyclonal 1:1000 Adiponectin Adiponectin antibody R & D Systems MAB1119 AB_2305045 Rat monoclonal 1:1000 AMPK α AMPK α antibody Cell Signaling 2532 AB_330331 Rabbit monoclonal 1:1000 pAMPK α pAMPK α antibody Cell Signaling 2535 AB_331250 Rabbit monoclonal 1:1000 Glut4 Glucose transporter 4 antibody Abcam ab65267 AB_1140009 Mouse monoclonal 1:1000 p38MAPK p38MAPK antibody Cell Signaling 9212S AB_330713 Rabbit polyclonal 1:1000 pp38MAPK pp38MAPK antibody Cell Signaling 9211S AB_331641 Rabbit polyclonal 1:1000 ERK1/2 ERK1/2 antibody Cell Signaling 9102S AB_330744 Rabbit polyclonal 1:1000 pERK1/2 pERK1/2 antibody Cell Signaling 9101S AB_331646 Rabbit polyclonal 1:1000 Myf5 Myf5 antibody Santa Cruz Biotech sc-302 AB_631994 Rabbit polyclonal 1:1000 Cd137 Cd137 antibody Abcam ab3169 AB_303572 Mouse monoclonal 1:1000 BMP7 BMP7 antibody Abcam ab54904 AB_940598 Mouse monoclonal 1:1000
Techniques: Modification, Recombinant, Expressing, Western Blot, Gene Expression
Journal: Nanomaterials
Article Title: Rapid Production of Carbon Nanotube Film for Bioelectronic Applications
doi: 10.3390/nano13111749
Figure Lengend Snippet: Raw CNT was synthesized ( a ) displays a graphic of the equipment used for CNT development by CVD in its simplest form. The technique requires passing a hydrocarbon vapor (usually for 15 to 60 min) through a tube furnace in which a catalyst material is present at sufficiently high temperature (500–1000 °C) to breakdown the hydrocarbon. When the system is cooled to room temperature, the CNTs that have grown over the catalyst may be collected. Heating a flask containing the liquid hydrocarbon (benzene, alcohol, etc.) causes it to evaporate, and the resulting vapor is then sucked into the reaction furnace through an inert gas. ( b ) Top-view scanning electron microscopic (SEM) images of continuously grown CNT films (scale bar 100 µm) with Morphological and electrical characterizations in various areas. ( c ) Raman spectra acquired at an excitation of 532 nm of the as-prepared carbon nanotube array. ( d ) Typical XRD analysis for CNTs. ( e ) Results of Energy dispersive spectroscopy (EDS) point analysis of CNT arrays.
Article Snippet: The diffraction pattern produced by the CNTs is analyzed using software such as
Techniques: Synthesized, Spectroscopy