ethylenediaminetetraacetic acid Millipore Search Results


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  • 95
    Millipore ethylenediaminetetraacetic acid disodium salt
    Sample preparation and instrumental analysis of varietal thiols in white and red wine through 4,4-dithiodipyridine (DTDP) derivatization. EDTA, <t>ethylenediaminetetraacetic</t> acid disodium salt; SPE, solid phase extraction; RT, room temperature; UPC 2 , ultraperformance convergence chromatography; MS/MS, tandem mass spectrometry; 3-MH: 3-mercaptohexanol; 3-MHA: 3-mercaptohexyl acetate; 4-MMP: 4-mercapto-4-methylpentane-2-one; FMT; furanmethanethiol; the DTDP appendix designates the respective derivative.
    Ethylenediaminetetraacetic Acid Disodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore edta
    TEM images of particles formed by various <t>DNA</t> samples upon condensation with the Tat-NLS peptide. ( A ) Condensates formed by the nicked-DNA duplexes of oligonucleotides N1 and N2. ( B ) Condensates formed by the gapped-DNA duplexes of oligonucleotides G1 and G2. ( C ) Condensates formed by the nicked-gapped-DNA duplex of oligonucleotides N1 and G2. ( D ) Condensates formed by 3kbDNA . ( E ) Condensates formed by 21mer duplex. For all samples, DNA was 15 μM in base pair, and was condensed by mixing with the Tat-NLS peptide at a charge ratio of 1:2 (DNA phosphate:cationic charged group of the peptide) in 5 mM Bis-Tris, 50 μM <t>EDTA</t> (pH 7.0). Scale bar is 100 nm.
    Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 32400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore ethylenediaminetetraacetic acid dipotassium salt dehydrate
    TEM images of particles formed by various <t>DNA</t> samples upon condensation with the Tat-NLS peptide. ( A ) Condensates formed by the nicked-DNA duplexes of oligonucleotides N1 and N2. ( B ) Condensates formed by the gapped-DNA duplexes of oligonucleotides G1 and G2. ( C ) Condensates formed by the nicked-gapped-DNA duplex of oligonucleotides N1 and G2. ( D ) Condensates formed by 3kbDNA . ( E ) Condensates formed by 21mer duplex. For all samples, DNA was 15 μM in base pair, and was condensed by mixing with the Tat-NLS peptide at a charge ratio of 1:2 (DNA phosphate:cationic charged group of the peptide) in 5 mM Bis-Tris, 50 μM <t>EDTA</t> (pH 7.0). Scale bar is 100 nm.
    Ethylenediaminetetraacetic Acid Dipotassium Salt Dehydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore ethylenediaminetetraacetic acid edta disodium salt dihydrate
    Flowchart showing the method of sample preparation for analyzing nucleotides in samples. TMP, thymidine 5′-monophosphate; SPE, solid phase extraction; <t>EDTA,</t> <t>ethylenediaminetetraacetic</t> acid; ISTD, internal standard.
    Ethylenediaminetetraacetic Acid Edta Disodium Salt Dihydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore ethylenediaminetetraacetic dianhydride edta
    Flowchart showing the method of sample preparation for analyzing nucleotides in samples. TMP, thymidine 5′-monophosphate; SPE, solid phase extraction; <t>EDTA,</t> <t>ethylenediaminetetraacetic</t> acid; ISTD, internal standard.
    Ethylenediaminetetraacetic Dianhydride Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore edta solution
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Edta Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 590 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore ethylenediaminetretraacetic acid
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylenediaminetretraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore ethylenediaminetetraacetic acid trisodium salt
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylenediaminetetraacetic Acid Trisodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ethylenediaminetetraacetate edta
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylenediaminetetraacetate Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore ethylenediaminetetraacetic acid tripotassium salt dihydrate edta
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylenediaminetetraacetic Acid Tripotassium Salt Dihydrate Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore ethylenediaminetetraacetic acid etda disodium salt
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylenediaminetetraacetic Acid Etda Disodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore ethylenediaminetetracetic acid
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylenediaminetetracetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Millipore ethylenediaminetetraacetic acid calcium disodium salt hydrate ca edta
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylenediaminetetraacetic Acid Calcium Disodium Salt Hydrate Ca Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Millipore ethylendiaminetetraacetic acid
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Ethylendiaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore dl dithiothreitol
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Dl Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore fe iii edta ethylenediaminetetraacetic acid iron iii sodium hydrate
    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × <t>PBS</t> solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM <t>EDTA</t> at scan rate 50 mV s −1 .
    Fe Iii Edta Ethylenediaminetetraacetic Acid Iron Iii Sodium Hydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore ethylenediaminetetraacetic acid edta disodium salt
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Ethylenediaminetetraacetic Acid Edta Disodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore ethylenediamineteraacetic acid
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Ethylenediamineteraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore 0 5x tris borate ethylenediaminetetraacetic acid
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    0 5x Tris Borate Ethylenediaminetetraacetic Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore trypsin ethylenediamminetetraacetate
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Trypsin Ethylenediamminetetraacetate, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore magnesium chloride
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Magnesium Chloride, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore disodium dihydrogen ethylenediaminetetraacetate dihydrate
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Disodium Dihydrogen Ethylenediaminetetraacetate Dihydrate, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore cyclophosphamide
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Cyclophosphamide, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore m edta
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    M Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 6525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore edta free
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Edta Free, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore edta ferric sodium salt
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Edta Ferric Sodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore ethylenediaminetetraacetic acid tetrasodium salt hydrate
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
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    79
    Millipore molecular grade 1m tris ethylenediaminetetraacetate edta 0 5 1 25 ml
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
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    97
    Merck KGaA edta
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Edta, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 729 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore ethylenediaminetetraacetic acid iron iii sodium salt fe iii edta
    Experimental diagram. Progressive demineralization each day. <t>EDTA</t> - <t>Ethylenediaminetetraacetic</t> Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.
    Ethylenediaminetetraacetic Acid Iron Iii Sodium Salt Fe Iii Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Sample preparation and instrumental analysis of varietal thiols in white and red wine through 4,4-dithiodipyridine (DTDP) derivatization. EDTA, ethylenediaminetetraacetic acid disodium salt; SPE, solid phase extraction; RT, room temperature; UPC 2 , ultraperformance convergence chromatography; MS/MS, tandem mass spectrometry; 3-MH: 3-mercaptohexanol; 3-MHA: 3-mercaptohexyl acetate; 4-MMP: 4-mercapto-4-methylpentane-2-one; FMT; furanmethanethiol; the DTDP appendix designates the respective derivative.

    Journal: Foods

    Article Title: Measuring Thiols in Single Cultivar South African Red Wines Using 4,4-Dithiodipyridine (DTDP) Derivatization and Ultraperformance Convergence Chromatography-Tandem Mass Spectrometry

    doi: 10.3390/foods7090138

    Figure Lengend Snippet: Sample preparation and instrumental analysis of varietal thiols in white and red wine through 4,4-dithiodipyridine (DTDP) derivatization. EDTA, ethylenediaminetetraacetic acid disodium salt; SPE, solid phase extraction; RT, room temperature; UPC 2 , ultraperformance convergence chromatography; MS/MS, tandem mass spectrometry; 3-MH: 3-mercaptohexanol; 3-MHA: 3-mercaptohexyl acetate; 4-MMP: 4-mercapto-4-methylpentane-2-one; FMT; furanmethanethiol; the DTDP appendix designates the respective derivative.

    Article Snippet: All reagents—3-MH, 6-mercapto-1-hexanol (6-MH), 3-MHA, 4-MMP, FMT, 98% DTDP, ethylenediaminetetraacetic acid disodium salt (EDTA–Na2 ), methanol, 96% ethanol, sodium hydroxide (NaOH), tartaric acid, anhydrous acetaldehyde ≥98%, and 37% hydrochloric acid (HCl)—were purchased from Sigma-Aldrich (Louisville, MO, USA) and the solid phase extraction (SPE) cartridges (Supelclean ENVI-18 SPE) from Supelco (Bellefonte, PA, USA ).

    Techniques: Sample Prep, Chromatography, Mass Spectrometry

    TEM images of particles formed by various DNA samples upon condensation with the Tat-NLS peptide. ( A ) Condensates formed by the nicked-DNA duplexes of oligonucleotides N1 and N2. ( B ) Condensates formed by the gapped-DNA duplexes of oligonucleotides G1 and G2. ( C ) Condensates formed by the nicked-gapped-DNA duplex of oligonucleotides N1 and G2. ( D ) Condensates formed by 3kbDNA . ( E ) Condensates formed by 21mer duplex. For all samples, DNA was 15 μM in base pair, and was condensed by mixing with the Tat-NLS peptide at a charge ratio of 1:2 (DNA phosphate:cationic charged group of the peptide) in 5 mM Bis-Tris, 50 μM EDTA (pH 7.0). Scale bar is 100 nm.

    Journal: Nucleic Acids Research

    Article Title: Condensation of oligonucleotides assembled into nicked and gapped duplexes: potential structures for oligonucleotide delivery

    doi: 10.1093/nar/gki156

    Figure Lengend Snippet: TEM images of particles formed by various DNA samples upon condensation with the Tat-NLS peptide. ( A ) Condensates formed by the nicked-DNA duplexes of oligonucleotides N1 and N2. ( B ) Condensates formed by the gapped-DNA duplexes of oligonucleotides G1 and G2. ( C ) Condensates formed by the nicked-gapped-DNA duplex of oligonucleotides N1 and G2. ( D ) Condensates formed by 3kbDNA . ( E ) Condensates formed by 21mer duplex. For all samples, DNA was 15 μM in base pair, and was condensed by mixing with the Tat-NLS peptide at a charge ratio of 1:2 (DNA phosphate:cationic charged group of the peptide) in 5 mM Bis-Tris, 50 μM EDTA (pH 7.0). Scale bar is 100 nm.

    Article Snippet: The linearized plasmid DNA was rinsed five times with 5 mM Bis-Tris and 50 μM EDTA (pH 7.0) using a Microcon YM-30 spin column (Millipore, Bedford, MA) to remove excess salt introduced during restriction digestion.

    Techniques: Transmission Electron Microscopy

    Condensation of 3kbDNA , nicked-DNA and a 21mer duplex by hexammine cobalt chloride, as monitored by light scattering. For each data point shown, DNA concentration was 7.5 μM in base pair (5 mM Bis-Tris, 50 μM EDTA, pH 7.0). Light-scattering intensities shown are averages from measurements taken over a 5 min period. Inset : 3kbDNA and nicked-DNA scattering intensities, as a function of hexammine cobalt chloride concentration, normalized to maximum observed intensity. Note : nicked-DNA condensation occurs at a lower hexammine cobalt chloride concentration than 3kbDNA .

    Journal: Nucleic Acids Research

    Article Title: Condensation of oligonucleotides assembled into nicked and gapped duplexes: potential structures for oligonucleotide delivery

    doi: 10.1093/nar/gki156

    Figure Lengend Snippet: Condensation of 3kbDNA , nicked-DNA and a 21mer duplex by hexammine cobalt chloride, as monitored by light scattering. For each data point shown, DNA concentration was 7.5 μM in base pair (5 mM Bis-Tris, 50 μM EDTA, pH 7.0). Light-scattering intensities shown are averages from measurements taken over a 5 min period. Inset : 3kbDNA and nicked-DNA scattering intensities, as a function of hexammine cobalt chloride concentration, normalized to maximum observed intensity. Note : nicked-DNA condensation occurs at a lower hexammine cobalt chloride concentration than 3kbDNA .

    Article Snippet: The linearized plasmid DNA was rinsed five times with 5 mM Bis-Tris and 50 μM EDTA (pH 7.0) using a Microcon YM-30 spin column (Millipore, Bedford, MA) to remove excess salt introduced during restriction digestion.

    Techniques: Concentration Assay

    TEM images of particles formed by various DNA samples upon condensation with hexammine cobalt chloride. ( A ) Condensates formed by the nicked-DNA duplexes of oligonucleotides N1 and N2. ( B ) Condensates formed by the gapped-DNA duplexes of oligonucleotides G1 and G2. ( C ) Condensates formed by the nicked-gapped-DNA duplex of oligonucleotides N1 and G2. ( D ) Condensates formed by 3kbDNA . For all samples, DNA was 15 μM in base pair, and condensed by mixing with an equal volume of 200 μM hexammine cobalt chloride in 5 mM Bis-Tris, 50 μM EDTA (pH 7.0). Scale bar is 100 nm.

    Journal: Nucleic Acids Research

    Article Title: Condensation of oligonucleotides assembled into nicked and gapped duplexes: potential structures for oligonucleotide delivery

    doi: 10.1093/nar/gki156

    Figure Lengend Snippet: TEM images of particles formed by various DNA samples upon condensation with hexammine cobalt chloride. ( A ) Condensates formed by the nicked-DNA duplexes of oligonucleotides N1 and N2. ( B ) Condensates formed by the gapped-DNA duplexes of oligonucleotides G1 and G2. ( C ) Condensates formed by the nicked-gapped-DNA duplex of oligonucleotides N1 and G2. ( D ) Condensates formed by 3kbDNA . For all samples, DNA was 15 μM in base pair, and condensed by mixing with an equal volume of 200 μM hexammine cobalt chloride in 5 mM Bis-Tris, 50 μM EDTA (pH 7.0). Scale bar is 100 nm.

    Article Snippet: The linearized plasmid DNA was rinsed five times with 5 mM Bis-Tris and 50 μM EDTA (pH 7.0) using a Microcon YM-30 spin column (Millipore, Bedford, MA) to remove excess salt introduced during restriction digestion.

    Techniques: Transmission Electron Microscopy

    Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Journal: International Journal of Nanomedicine

    Article Title: Enhanced uptake and transport of (+)-catechin and (-)-epigallocatechin gallate in niosomal formulation by human intestinal Caco-2 cells

    doi: 10.2147/IJN.S59331

    Figure Lengend Snippet: Transport of catechin, EGCG, and their niosomes by human intestinal Caco-2 cells. Notes: Schematic diagram of transcellular transport and different transporters in Caco-2 cells ( A ); effect of concentration on the flux of catechin, EGCG, and their niosomes (0, 10, 20, 50, and 100 μg/mL) through Caco-2 monolayer from apical to basolateral chamber ( B ); effect of temperature (4°C and 37°C), ATP inhibitor (sodium azide), P-gp inhibitor (verapamil), MRP2 inhibitor (MK-571) and absorption enhancer (EDTA) on transport of 100 μg/mL different drugs after 6 hours ( C ). Data are presented as mean ± SD (n=3). Abbreviations: EDTA, ethylenediaminetetraacetic acid; EGCG, (−)-epigallocatechin gallate; MRP2, multidrug resistance-associated protein 2; P-gp, permeability glycoprotein; SD, standard deviation.

    Article Snippet: Chemicals and reagents Catechin, EGCG, sorbitan monostearate (Span 60), CH, dihexadecyl phosphate, fluorescein isothiocyanate (FITC), sulforhodamine B (SRB), fluorescein sodium salt, ascorbic acid (AA), sodium azide, verapamil, 5-(3-(2-(7-chloroquinolin-2-yl)ethenyl)phenyl)-8-dimethylcarbamyl-4,6-dithiaoctanoic acid sodium salt hydrate (MK-571), and ethylenediaminetetraacetic acid (EDTA) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Concentration Assay, Permeability, Standard Deviation

    Flowchart showing the method of sample preparation for analyzing nucleotides in samples. TMP, thymidine 5′-monophosphate; SPE, solid phase extraction; EDTA, ethylenediaminetetraacetic acid; ISTD, internal standard.

    Journal: Food Science of Animal Resources

    Article Title: Nucleotide Analysis in Korean Dairy Products Using High- Performance Liquid Chromatography with Diode Array Detector

    doi: 10.5851/kosfa.2019.e7

    Figure Lengend Snippet: Flowchart showing the method of sample preparation for analyzing nucleotides in samples. TMP, thymidine 5′-monophosphate; SPE, solid phase extraction; EDTA, ethylenediaminetetraacetic acid; ISTD, internal standard.

    Article Snippet: Water and methanol used for all experiments were purchased from Budick and Jackson (Ulsan, Korea), potassium bromide and ethylenediaminetetraacetic acid (EDTA) disodium salt dihydrate were purchased from Sigma, potassium dihydrogen phosphate was purchased from Kanto (Tokyo, Japan), phosphoric acid was purchased from Junsei (Tokyo, Japan), and potassium hydroxide and sodium chloride were purchased from Junsei.

    Techniques: Sample Prep

    Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1 .

    Journal: Biosensors & bioelectronics

    Article Title: An electrochemical immunosensing method for detecting melanoma cells

    doi: 10.1016/j.bios.2015.01.022

    Figure Lengend Snippet: Schematic of (A) fabrication of the immunosensor and (B) reaction occurring at the working electrode surface in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1 .

    Article Snippet: Cells were dislodged from the surface of the culture dish by using 0.25% Trypsin–EDTA (Life Technologies, Grand Island, NY), or 2% EDTA solution in 1 × PBS (Sigma, St. Louis, MO), or a cell scraper (Sarstedt, Newton, NC).

    Techniques:

    (A) Typical cyclic voltammograms of (a) bare SPE (b) PPy/SPE, (c) n-SiNPs/PPy/SPE and (d) MC1R-Ab/n-SiNPs/PPy/SPE in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1

    Journal: Biosensors & bioelectronics

    Article Title: An electrochemical immunosensing method for detecting melanoma cells

    doi: 10.1016/j.bios.2015.01.022

    Figure Lengend Snippet: (A) Typical cyclic voltammograms of (a) bare SPE (b) PPy/SPE, (c) n-SiNPs/PPy/SPE and (d) MC1R-Ab/n-SiNPs/PPy/SPE in 1 × PBS solution at pH 7.4 containing 1 mM[Fe(CN) 6 ] 3− , 0.1 M KCl, and 100 μM EDTA at scan rate 50 mV s −1

    Article Snippet: Cells were dislodged from the surface of the culture dish by using 0.25% Trypsin–EDTA (Life Technologies, Grand Island, NY), or 2% EDTA solution in 1 × PBS (Sigma, St. Louis, MO), or a cell scraper (Sarstedt, Newton, NC).

    Techniques:

    Experimental diagram. Progressive demineralization each day. EDTA - Ethylenediaminetetraacetic Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.

    Journal: Scientific Reports

    Article Title: Monitoring bone changes due to calcium, magnesium, and phosphorus loss in rat femurs using Quantitative Ultrasound

    doi: 10.1038/s41598-018-30327-7

    Figure Lengend Snippet: Experimental diagram. Progressive demineralization each day. EDTA - Ethylenediaminetetraacetic Acid solutions; QUS - Quantitative Ultrasound; QCT - Quantitative Computed Tomography; ICP OES - coupled plasma optical emission spectrometry. Day 0 - integer bone. Demineralization occurs from Day 1 to Day 5.

    Article Snippet: The demineralization protocol consisted of immersing the rat femur samples in a 25-mL solution of Ethylenediaminetetraacetic Acid (EDTA) disodium salt (Sigma-Aldrich®, Missouri, USA) at pH = 8 and 0.376 M concentration for 24 h at 25.0 ± 1.5 °C, and a new solution and new reservoir were used each day .

    Techniques: Computed Tomography