ethanol etoh Search Results


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  • 99
    Millipore ethanol etoh
    Atomic force microscopy height images of a phenytoin sample prepared from <t>EtOH</t> solution (a) or from <t>THF</t> solution (b). A reflection mode optical microscopy image taken under crossed polarizers of the THF sample is shown in (c). The dotted lines indicate the grain boundaries of the surface-induced phase, and arrows indicate the position of trenches in between two common areas.
    Ethanol Etoh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    PHARMCO-AAPER ethyl alcohol etoh
    GA, <t>EtG</t> and <t>EtOH</t> all cause a significant increase HEK-TLR4 cell SEAP reporter expression. GA caused a significant increase relative to vehicle at 10 and 100 μM concentrations (F=19.16 p
    Ethyl Alcohol Etoh, supplied by PHARMCO-AAPER, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore absolute ethanol etoh
    GA, <t>EtG</t> and <t>EtOH</t> all cause a significant increase HEK-TLR4 cell SEAP reporter expression. GA caused a significant increase relative to vehicle at 10 and 100 μM concentrations (F=19.16 p
    Absolute Ethanol Etoh, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Avantor anhydrous ethyl alcohol etoh
    GA, <t>EtG</t> and <t>EtOH</t> all cause a significant increase HEK-TLR4 cell SEAP reporter expression. GA caused a significant increase relative to vehicle at 10 and 100 μM concentrations (F=19.16 p
    Anhydrous Ethyl Alcohol Etoh, supplied by Avantor, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore proof ethyl alcohol etoh
    GA, <t>EtG</t> and <t>EtOH</t> all cause a significant increase HEK-TLR4 cell SEAP reporter expression. GA caused a significant increase relative to vehicle at 10 and 100 μM concentrations (F=19.16 p
    Proof Ethyl Alcohol Etoh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore ethyl alcohol etoh pbs
    GA, <t>EtG</t> and <t>EtOH</t> all cause a significant increase HEK-TLR4 cell SEAP reporter expression. GA caused a significant increase relative to vehicle at 10 and 100 μM concentrations (F=19.16 p
    Ethyl Alcohol Etoh Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Avantor ethanol etoh
    HPTLC chromatogram of PE, <t>EtOAc,</t> and <t>EtOH</t> extracts of B. acutangula leaf, (PE- Petroleum ether extract, EtOAc- Ethyl acetate extract, EtOH- Ethanol extract)
    Ethanol Etoh, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher ethanol etoh
    HPTLC chromatogram of PE, <t>EtOAc,</t> and <t>EtOH</t> extracts of B. acutangula leaf, (PE- Petroleum ether extract, EtOAc- Ethyl acetate extract, EtOH- Ethanol extract)
    Ethanol Etoh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Fisher Scientific ethanol etoh
    Schematic Overview of Stepwise Polymer Brush Formation from Gold Surfaces and Dynabeads Notes: (a) MBMP, <t>EtOH,</t> 24 h, rt; (b and d) for SBMAA-3, SBMAA-2, CBMAA-2, and PCMA-2: Cu(I)/Cu(II) (90/10), 2,2′-bipyridyl, isopropanol/water (20/80), 12 min, rt, for HPMAA: Cu(I)/Cu(II) (90/10), Me 4 Cyclam, EtOH, 2.5 h, 30 °C; (c) α-bromoisobutyryl bromide, DIPEA, <t>DCM,</t> overnight, rt.
    Ethanol Etoh, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co ethanol etoh
    Schematic Overview of Stepwise Polymer Brush Formation from Gold Surfaces and Dynabeads Notes: (a) MBMP, <t>EtOH,</t> 24 h, rt; (b and d) for SBMAA-3, SBMAA-2, CBMAA-2, and PCMA-2: Cu(I)/Cu(II) (90/10), 2,2′-bipyridyl, isopropanol/water (20/80), 12 min, rt, for HPMAA: Cu(I)/Cu(II) (90/10), Me 4 Cyclam, EtOH, 2.5 h, 30 °C; (c) α-bromoisobutyryl bromide, DIPEA, <t>DCM,</t> overnight, rt.
    Ethanol Etoh, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Decon Laboratories ethanol etoh
    Schematic Overview of Stepwise Polymer Brush Formation from Gold Surfaces and Dynabeads Notes: (a) MBMP, <t>EtOH,</t> 24 h, rt; (b and d) for SBMAA-3, SBMAA-2, CBMAA-2, and PCMA-2: Cu(I)/Cu(II) (90/10), 2,2′-bipyridyl, isopropanol/water (20/80), 12 min, rt, for HPMAA: Cu(I)/Cu(II) (90/10), Me 4 Cyclam, EtOH, 2.5 h, 30 °C; (c) α-bromoisobutyryl bromide, DIPEA, <t>DCM,</t> overnight, rt.
    Ethanol Etoh, supplied by Decon Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sinopharm ethanol etoh
    (a) The in vitro release profile of RM from microspheres prepared by W/O/W double emulsion–solvent evaporation method (MS-B) and W/O/O emulsion-phase separation method with the drug loading of 7.20% (MS-E1), 17.34% (MS-E2), 26.42% (MS-E3) and 30.12% (MS-optimal formulation). (b) and (c) The effect of solvent type and volume in gel matrix solution on the in vitro release of RM-microsphere-Gel in situ forming implant, 15% <t>EtOH</t> (S1), 20% EtOH (S2), 25% EtOH (S3), 25% <t>NMP</t> (S4), 30% NMP (S5), 40% NMP (S6). (d) The in vitro release profile of RM from in situ gel with the solvent of 15% EtOH (S7) and 25% NMP (S8). Graphs symbolize mean ± SD. ( n = 3).
    Ethanol Etoh, supplied by Sinopharm, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Gold Shield Distributors ethanol etoh
    Ligand-dependent transformation and DNA binding of guinea pig hepatic cytosolic AhR by <t>DMSO,</t> <t>ETOH,</t> and water extracts of newspaper inks. Guinea pig hepatic cystosol (8 mg/ml in HEDG) was incubated with DMSO (2.0%), TCDD (20nM), or a DMSO (D), ethanol (E), or water (W) extract of the indicated ink (1:10 wt/vol) for 2 h at room temperature. Aliquots of each reaction were incubated with [ 32 P]DRE and protein:DNA complexes resolved by gel retardation analysis as described under “Materials and Methods.” The amount of induced protein-[ 32 P]DRE complex was determined by phosphorimager analysis and values represent the mean ± SD of the amount of ink extract induced protein-[ 32 P]DRE complex formation expressed relative to the maximal amount of TCDD-induced protein-[ 32 P]DRE complex.
    Ethanol Etoh, supplied by Gold Shield Distributors, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM ethanol etoh
    Ligand-dependent transformation and DNA binding of guinea pig hepatic cytosolic AhR by <t>DMSO,</t> <t>ETOH,</t> and water extracts of newspaper inks. Guinea pig hepatic cystosol (8 mg/ml in HEDG) was incubated with DMSO (2.0%), TCDD (20nM), or a DMSO (D), ethanol (E), or water (W) extract of the indicated ink (1:10 wt/vol) for 2 h at room temperature. Aliquots of each reaction were incubated with [ 32 P]DRE and protein:DNA complexes resolved by gel retardation analysis as described under “Materials and Methods.” The amount of induced protein-[ 32 P]DRE complex was determined by phosphorimager analysis and values represent the mean ± SD of the amount of ink extract induced protein-[ 32 P]DRE complex formation expressed relative to the maximal amount of TCDD-induced protein-[ 32 P]DRE complex.
    Ethanol Etoh, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA ethanol etoh
    Total phenolic contents of <t>MF_EtOH</t> and <t>MF_EtOAc</t> extracts. MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers; GAE, gallic acid equivalents.
    Ethanol Etoh, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethanol etoh/product/Merck KGaA
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    91
    Sinopharm absolute ethyl alcohol etoh
    Total phenolic contents of <t>MF_EtOH</t> and <t>MF_EtOAc</t> extracts. MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers; GAE, gallic acid equivalents.
    Absolute Ethyl Alcohol Etoh, supplied by Sinopharm, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    PHARMCO-AAPER anhydrous ethyl alcohol etoh
    Total phenolic contents of <t>MF_EtOH</t> and <t>MF_EtOAc</t> extracts. MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers; GAE, gallic acid equivalents.
    Anhydrous Ethyl Alcohol Etoh, supplied by PHARMCO-AAPER, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 2 article reviews
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    86
    Avantor 200proof ethanol etoh
    Total phenolic contents of <t>MF_EtOH</t> and <t>MF_EtOAc</t> extracts. MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers; GAE, gallic acid equivalents.
    200proof Ethanol Etoh, supplied by Avantor, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Atomic force microscopy height images of a phenytoin sample prepared from EtOH solution (a) or from THF solution (b). A reflection mode optical microscopy image taken under crossed polarizers of the THF sample is shown in (c). The dotted lines indicate the grain boundaries of the surface-induced phase, and arrows indicate the position of trenches in between two common areas.

    Journal: Crystal Growth & Design

    Article Title: Surface-Induced Polymorphism as a Tool for Enhanced Dissolution: The Example of Phenytoin

    doi: 10.1021/acs.cgd.5b01002

    Figure Lengend Snippet: Atomic force microscopy height images of a phenytoin sample prepared from EtOH solution (a) or from THF solution (b). A reflection mode optical microscopy image taken under crossed polarizers of the THF sample is shown in (c). The dotted lines indicate the grain boundaries of the surface-induced phase, and arrows indicate the position of trenches in between two common areas.

    Article Snippet: Spectral grade tetrahydrofuran (THF) (Fluka, Germany) and ethanol (EtOH) (Aldrich, Germany) were used as solvents.

    Techniques: Microscopy

    Specular X-ray diffraction scans of phenytoin samples prepared from THF (bottom) and EtOH (center) solutions compared to a calculated spectra of an ideal powder of the bulk polymorph (top).

    Journal: Crystal Growth & Design

    Article Title: Surface-Induced Polymorphism as a Tool for Enhanced Dissolution: The Example of Phenytoin

    doi: 10.1021/acs.cgd.5b01002

    Figure Lengend Snippet: Specular X-ray diffraction scans of phenytoin samples prepared from THF (bottom) and EtOH (center) solutions compared to a calculated spectra of an ideal powder of the bulk polymorph (top).

    Article Snippet: Spectral grade tetrahydrofuran (THF) (Fluka, Germany) and ethanol (EtOH) (Aldrich, Germany) were used as solvents.

    Techniques:

    GA, EtG and EtOH all cause a significant increase HEK-TLR4 cell SEAP reporter expression. GA caused a significant increase relative to vehicle at 10 and 100 μM concentrations (F=19.16 p

    Journal: Brain, behavior, and immunity

    Article Title: Glucuronic acid and the ethanol metabolite ethyl-glucuronide cause Toll-like receptor 4 activation and enhanced pain

    doi: 10.1016/j.bbi.2013.01.005

    Figure Lengend Snippet: GA, EtG and EtOH all cause a significant increase HEK-TLR4 cell SEAP reporter expression. GA caused a significant increase relative to vehicle at 10 and 100 μM concentrations (F=19.16 p

    Article Snippet: Ethyl glucuronide (EtG) was purchased from Aurora Analytics (Baltimore, MD) and ethyl alcohol (EtOH) from Pharmco (Brookfield, CT).

    Techniques: Expressing

    HPTLC chromatogram of PE, EtOAc, and EtOH extracts of B. acutangula leaf, (PE- Petroleum ether extract, EtOAc- Ethyl acetate extract, EtOH- Ethanol extract)

    Journal: International Journal of Ayurveda Research

    Article Title: Pharmacognostical evaluation of Barringtonia acutangula leaf

    doi: 10.4103/0974-7788.83189

    Figure Lengend Snippet: HPTLC chromatogram of PE, EtOAc, and EtOH extracts of B. acutangula leaf, (PE- Petroleum ether extract, EtOAc- Ethyl acetate extract, EtOH- Ethanol extract)

    Article Snippet: Solvents viz ., 95% Ethanol (EtOH), Petroleum ether (PE), Ethyl acetate (EtOAc) and reagents such as phloroglucinol, glycerin, hydrochloric acid, chloral hydrate, and sodium hydroxide were procured from Ranbaxy Fine Chemicals Ltd., Mumbai, India.

    Techniques: High Performance Thin Layer Chromatography

    Schematic Overview of Stepwise Polymer Brush Formation from Gold Surfaces and Dynabeads Notes: (a) MBMP, EtOH, 24 h, rt; (b and d) for SBMAA-3, SBMAA-2, CBMAA-2, and PCMA-2: Cu(I)/Cu(II) (90/10), 2,2′-bipyridyl, isopropanol/water (20/80), 12 min, rt, for HPMAA: Cu(I)/Cu(II) (90/10), Me 4 Cyclam, EtOH, 2.5 h, 30 °C; (c) α-bromoisobutyryl bromide, DIPEA, DCM, overnight, rt.

    Journal: Langmuir

    Article Title: Systematic Comparison of Zwitterionic and Non-Zwitterionic Antifouling Polymer Brushes on a Bead-Based Platform

    doi: 10.1021/acs.langmuir.8b01832

    Figure Lengend Snippet: Schematic Overview of Stepwise Polymer Brush Formation from Gold Surfaces and Dynabeads Notes: (a) MBMP, EtOH, 24 h, rt; (b and d) for SBMAA-3, SBMAA-2, CBMAA-2, and PCMA-2: Cu(I)/Cu(II) (90/10), 2,2′-bipyridyl, isopropanol/water (20/80), 12 min, rt, for HPMAA: Cu(I)/Cu(II) (90/10), Me 4 Cyclam, EtOH, 2.5 h, 30 °C; (c) α-bromoisobutyryl bromide, DIPEA, DCM, overnight, rt.

    Article Snippet: Acetone (HPLC grade; 99.9%, BIOSOLVE BV), N ,N -dimethylformamide (DMF) (Fisher Scientific), methanol (MeOH) (HPLC grade, Rathburn Chemicals Ltd.), dichloromethane (DCM) (VWR International S.A.S.), isopropanol (HPLC grade; BIOSOLVE), absolute ethanol (EtOH) (Fisher Scientific), dry tetrahydrofuran (Sigma-Aldrich), and deionized water produced with a Milli-Q Integral 3 system (Millipore, Molsheim, France) were used as solvents.

    Techniques:

    (a) The in vitro release profile of RM from microspheres prepared by W/O/W double emulsion–solvent evaporation method (MS-B) and W/O/O emulsion-phase separation method with the drug loading of 7.20% (MS-E1), 17.34% (MS-E2), 26.42% (MS-E3) and 30.12% (MS-optimal formulation). (b) and (c) The effect of solvent type and volume in gel matrix solution on the in vitro release of RM-microsphere-Gel in situ forming implant, 15% EtOH (S1), 20% EtOH (S2), 25% EtOH (S3), 25% NMP (S4), 30% NMP (S5), 40% NMP (S6). (d) The in vitro release profile of RM from in situ gel with the solvent of 15% EtOH (S7) and 25% NMP (S8). Graphs symbolize mean ± SD. ( n = 3).

    Journal: Drug Delivery

    Article Title: Preparation and evaluation of injectable Rasagiline mesylate dual-controlled drug delivery system for the treatment of Parkinson’s disease

    doi: 10.1080/10717544.2017.1419514

    Figure Lengend Snippet: (a) The in vitro release profile of RM from microspheres prepared by W/O/W double emulsion–solvent evaporation method (MS-B) and W/O/O emulsion-phase separation method with the drug loading of 7.20% (MS-E1), 17.34% (MS-E2), 26.42% (MS-E3) and 30.12% (MS-optimal formulation). (b) and (c) The effect of solvent type and volume in gel matrix solution on the in vitro release of RM-microsphere-Gel in situ forming implant, 15% EtOH (S1), 20% EtOH (S2), 25% EtOH (S3), 25% NMP (S4), 30% NMP (S5), 40% NMP (S6). (d) The in vitro release profile of RM from in situ gel with the solvent of 15% EtOH (S7) and 25% NMP (S8). Graphs symbolize mean ± SD. ( n = 3).

    Article Snippet: Ethanol (EtOH), n -methylpyrrolidone (NMP), dichloromethane (CH2 Cl2 ), and n-heptane were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China.

    Techniques: In Vitro, Evaporation, Mass Spectrometry, In Situ

    Ligand-dependent transformation and DNA binding of guinea pig hepatic cytosolic AhR by DMSO, ETOH, and water extracts of newspaper inks. Guinea pig hepatic cystosol (8 mg/ml in HEDG) was incubated with DMSO (2.0%), TCDD (20nM), or a DMSO (D), ethanol (E), or water (W) extract of the indicated ink (1:10 wt/vol) for 2 h at room temperature. Aliquots of each reaction were incubated with [ 32 P]DRE and protein:DNA complexes resolved by gel retardation analysis as described under “Materials and Methods.” The amount of induced protein-[ 32 P]DRE complex was determined by phosphorimager analysis and values represent the mean ± SD of the amount of ink extract induced protein-[ 32 P]DRE complex formation expressed relative to the maximal amount of TCDD-induced protein-[ 32 P]DRE complex.

    Journal: Toxicological sciences : an official journal of the Society of Toxicology

    Article Title: Newspapers and Newspaper Ink Contain Agonists for the Ah Receptor

    doi: 10.1093/toxsci/kfn011

    Figure Lengend Snippet: Ligand-dependent transformation and DNA binding of guinea pig hepatic cytosolic AhR by DMSO, ETOH, and water extracts of newspaper inks. Guinea pig hepatic cystosol (8 mg/ml in HEDG) was incubated with DMSO (2.0%), TCDD (20nM), or a DMSO (D), ethanol (E), or water (W) extract of the indicated ink (1:10 wt/vol) for 2 h at room temperature. Aliquots of each reaction were incubated with [ 32 P]DRE and protein:DNA complexes resolved by gel retardation analysis as described under “Materials and Methods.” The amount of induced protein-[ 32 P]DRE complex was determined by phosphorimager analysis and values represent the mean ± SD of the amount of ink extract induced protein-[ 32 P]DRE complex formation expressed relative to the maximal amount of TCDD-induced protein-[ 32 P]DRE complex.

    Article Snippet: Dimethyl sulfoxide (DMSO) was purchased from Sigma (St Louis, MO) and ethanol (ETOH) from GoldShield Chemical (Hayward, CA).

    Techniques: Transformation Assay, Binding Assay, Incubation, Electrophoretic Mobility Shift Assay

    Competitive binding of DMSO and ETOH extracts of printed and virgin newspaper to guinea pig hepatic cytosolic AhR. Guinea pig hepatic cytosol (2 mg/ml) was incubated with 2nM [ 3 H]TCDD in the absence or presence of 100-fold excess TCDF, or an aliquot (1:100 vol/vol) of DMSO or ethanol extract of printed or virgin newspaper for 2 h at room temperature. [ 3 H]TCDD specific binding was determined using the hydroxyapatite binding assay as described in the “Materials and Methods.” Data are presented as a mean percent displacement ± SD of [ 3 H]TCDD specific binding of at least triplicate incubations. All extracts significantly displaced [ 3 H]TCDD specific binding ( p

    Journal: Toxicological sciences : an official journal of the Society of Toxicology

    Article Title: Newspapers and Newspaper Ink Contain Agonists for the Ah Receptor

    doi: 10.1093/toxsci/kfn011

    Figure Lengend Snippet: Competitive binding of DMSO and ETOH extracts of printed and virgin newspaper to guinea pig hepatic cytosolic AhR. Guinea pig hepatic cytosol (2 mg/ml) was incubated with 2nM [ 3 H]TCDD in the absence or presence of 100-fold excess TCDF, or an aliquot (1:100 vol/vol) of DMSO or ethanol extract of printed or virgin newspaper for 2 h at room temperature. [ 3 H]TCDD specific binding was determined using the hydroxyapatite binding assay as described in the “Materials and Methods.” Data are presented as a mean percent displacement ± SD of [ 3 H]TCDD specific binding of at least triplicate incubations. All extracts significantly displaced [ 3 H]TCDD specific binding ( p

    Article Snippet: Dimethyl sulfoxide (DMSO) was purchased from Sigma (St Louis, MO) and ethanol (ETOH) from GoldShield Chemical (Hayward, CA).

    Techniques: Binding Assay, Incubation

    Transient induction of AhR-dependent luciferase reporter gene expression by DMSO and ETOH extracts of printed newspaper, virgin paper, and black ink. Recombinant mouse hepatoma (H1L6.1c2) cells were plated (75,000 cells/well) in a 96-well microplate and incubated overnight at 37°C followed by treatment with solvent control (1.0%), TCDD (1nM), DMSO or ethanol extract of printed or virgin newspaper (1:10 w/v), or of black newspaper ink (1:100 wt/vol), further incubation for 24 h at 37°C followed by analysis of luciferase activity. Data are presented as a mean percent ± SD of the maximal induction obtained with 1nM TCDD and represent the results of at least triplicate incubations. At 24 h, luciferase activity of all paper and ink incubations (except for DMSO newspaper 1) were significantly less that that observed at 4 h of incubations ( p

    Journal: Toxicological sciences : an official journal of the Society of Toxicology

    Article Title: Newspapers and Newspaper Ink Contain Agonists for the Ah Receptor

    doi: 10.1093/toxsci/kfn011

    Figure Lengend Snippet: Transient induction of AhR-dependent luciferase reporter gene expression by DMSO and ETOH extracts of printed newspaper, virgin paper, and black ink. Recombinant mouse hepatoma (H1L6.1c2) cells were plated (75,000 cells/well) in a 96-well microplate and incubated overnight at 37°C followed by treatment with solvent control (1.0%), TCDD (1nM), DMSO or ethanol extract of printed or virgin newspaper (1:10 w/v), or of black newspaper ink (1:100 wt/vol), further incubation for 24 h at 37°C followed by analysis of luciferase activity. Data are presented as a mean percent ± SD of the maximal induction obtained with 1nM TCDD and represent the results of at least triplicate incubations. At 24 h, luciferase activity of all paper and ink incubations (except for DMSO newspaper 1) were significantly less that that observed at 4 h of incubations ( p

    Article Snippet: Dimethyl sulfoxide (DMSO) was purchased from Sigma (St Louis, MO) and ethanol (ETOH) from GoldShield Chemical (Hayward, CA).

    Techniques: Luciferase, Expressing, Recombinant, Incubation, Activity Assay

    Induction of AhR-dependent luciferase reporter gene expression by DMSO, ETOH, and distilled water extracts of printed newspaper, virgin paper, and black ink. Stably transfected guinea pig adenocarcinoma (G16L1.1c8) cells (A) or mouse hepatoma (H1L1.1c2) cells (B) were plated (75,000 cells/well) in a 96-well microplate and incubated overnight at 37°C followed by treatment with solvent control (1.0%), TCDD (1nM), or DMSO, ethanol, or distilled water extract of printed or virgin newspaper (1:10 wt/vol), or of black newspaper ink (1:1 wt/vol) for 4 h at 37°C. The papers vary by percentage of recycled material they contain, 40% and 100% for newspapers 1 and 2, respectively. Data are presented as a mean percent ± SD of the maximal induction obtained with 1nM TCDD and represent the results of triplicate incubations. All treatments induced luciferase activity significantly above the solvent control ( p

    Journal: Toxicological sciences : an official journal of the Society of Toxicology

    Article Title: Newspapers and Newspaper Ink Contain Agonists for the Ah Receptor

    doi: 10.1093/toxsci/kfn011

    Figure Lengend Snippet: Induction of AhR-dependent luciferase reporter gene expression by DMSO, ETOH, and distilled water extracts of printed newspaper, virgin paper, and black ink. Stably transfected guinea pig adenocarcinoma (G16L1.1c8) cells (A) or mouse hepatoma (H1L1.1c2) cells (B) were plated (75,000 cells/well) in a 96-well microplate and incubated overnight at 37°C followed by treatment with solvent control (1.0%), TCDD (1nM), or DMSO, ethanol, or distilled water extract of printed or virgin newspaper (1:10 wt/vol), or of black newspaper ink (1:1 wt/vol) for 4 h at 37°C. The papers vary by percentage of recycled material they contain, 40% and 100% for newspapers 1 and 2, respectively. Data are presented as a mean percent ± SD of the maximal induction obtained with 1nM TCDD and represent the results of triplicate incubations. All treatments induced luciferase activity significantly above the solvent control ( p

    Article Snippet: Dimethyl sulfoxide (DMSO) was purchased from Sigma (St Louis, MO) and ethanol (ETOH) from GoldShield Chemical (Hayward, CA).

    Techniques: Luciferase, Expressing, Stable Transfection, Transfection, Incubation, Activity Assay

    Ligand-dependent transformation and DNA binding of guinea pig hepatic cytosolic AhR by DMSO, ETOH, and water extracts of printed newspaper and virgin paper containing different amounts of recycled material. Guinea pig hepatic cystosol (8 mg/ml in HEDG) was incubated with DMSO (2.0%), TCDD (T; 20nM), or a DMSO (D), ethanol (E), or water (W) extract of printed or virgin (unprinted) newspaper (1:10 wt/vol) containing 40% (NP1) or 100% (NP2) recycled paper products for 2 h at room temperature. Aliquots of each reaction were incubated with [ 32 P]DRE and protein:DNA complexes resolved by gel retardation analysis as described under “Materials and Methods.” The arrow indicates the position of the induced AhR:ARNT:DRE complex.

    Journal: Toxicological sciences : an official journal of the Society of Toxicology

    Article Title: Newspapers and Newspaper Ink Contain Agonists for the Ah Receptor

    doi: 10.1093/toxsci/kfn011

    Figure Lengend Snippet: Ligand-dependent transformation and DNA binding of guinea pig hepatic cytosolic AhR by DMSO, ETOH, and water extracts of printed newspaper and virgin paper containing different amounts of recycled material. Guinea pig hepatic cystosol (8 mg/ml in HEDG) was incubated with DMSO (2.0%), TCDD (T; 20nM), or a DMSO (D), ethanol (E), or water (W) extract of printed or virgin (unprinted) newspaper (1:10 wt/vol) containing 40% (NP1) or 100% (NP2) recycled paper products for 2 h at room temperature. Aliquots of each reaction were incubated with [ 32 P]DRE and protein:DNA complexes resolved by gel retardation analysis as described under “Materials and Methods.” The arrow indicates the position of the induced AhR:ARNT:DRE complex.

    Article Snippet: Dimethyl sulfoxide (DMSO) was purchased from Sigma (St Louis, MO) and ethanol (ETOH) from GoldShield Chemical (Hayward, CA).

    Techniques: Transformation Assay, Binding Assay, Incubation, Electrophoretic Mobility Shift Assay

    Total phenolic contents of MF_EtOH and MF_EtOAc extracts. MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers; GAE, gallic acid equivalents.

    Journal: Experimental and Therapeutic Medicine

    Article Title: In vitro studies on the cytotoxicity, and elastase and tyrosinase inhibitory activities of marigold (Tagetes erecta L.) flower extracts

    doi: 10.3892/etm.2013.1373

    Figure Lengend Snippet: Total phenolic contents of MF_EtOH and MF_EtOAc extracts. MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers; GAE, gallic acid equivalents.

    Article Snippet: Ethanol (EtOH), ethyl acetate (EtOAc), dimethylsulfoxide (DMSO) and sodium dodecyl sulfate (SDS) were purchased from Merck KGaA (Darmstadt, Germany).

    Techniques:

    Viability of Caco-2 cells following treatment with MF_EtOH and MF_EtOAc extracts compared with that of the control group (n=10). MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers.

    Journal: Experimental and Therapeutic Medicine

    Article Title: In vitro studies on the cytotoxicity, and elastase and tyrosinase inhibitory activities of marigold (Tagetes erecta L.) flower extracts

    doi: 10.3892/etm.2013.1373

    Figure Lengend Snippet: Viability of Caco-2 cells following treatment with MF_EtOH and MF_EtOAc extracts compared with that of the control group (n=10). MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers.

    Article Snippet: Ethanol (EtOH), ethyl acetate (EtOAc), dimethylsulfoxide (DMSO) and sodium dodecyl sulfate (SDS) were purchased from Merck KGaA (Darmstadt, Germany).

    Techniques:

    Tyrosinase inhibitory activity of (A) MF_EtOH and (B) MF_EtOAc extracts and (C) kojic acid (n=3). MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers.

    Journal: Experimental and Therapeutic Medicine

    Article Title: In vitro studies on the cytotoxicity, and elastase and tyrosinase inhibitory activities of marigold (Tagetes erecta L.) flower extracts

    doi: 10.3892/etm.2013.1373

    Figure Lengend Snippet: Tyrosinase inhibitory activity of (A) MF_EtOH and (B) MF_EtOAc extracts and (C) kojic acid (n=3). MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers.

    Article Snippet: Ethanol (EtOH), ethyl acetate (EtOAc), dimethylsulfoxide (DMSO) and sodium dodecyl sulfate (SDS) were purchased from Merck KGaA (Darmstadt, Germany).

    Techniques: Activity Assay

    Viability of H460 cells following treatment with MF_EtOH and MF_EtOAc extracts compared with the control group (n=10). MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers.

    Journal: Experimental and Therapeutic Medicine

    Article Title: In vitro studies on the cytotoxicity, and elastase and tyrosinase inhibitory activities of marigold (Tagetes erecta L.) flower extracts

    doi: 10.3892/etm.2013.1373

    Figure Lengend Snippet: Viability of H460 cells following treatment with MF_EtOH and MF_EtOAc extracts compared with the control group (n=10). MF_EtOH, ethanol extract of marigold flowers; MF_EtOAc, ethyl acetate extract of marigold flowers.

    Article Snippet: Ethanol (EtOH), ethyl acetate (EtOAc), dimethylsulfoxide (DMSO) and sodium dodecyl sulfate (SDS) were purchased from Merck KGaA (Darmstadt, Germany).

    Techniques:

    Elastase inhibition activity of MF_EtOH (250 μg/ml) and MF_EtOAc (125 μg/ml) extracts compared with that of the negative control (n=3). a P

    Journal: Experimental and Therapeutic Medicine

    Article Title: In vitro studies on the cytotoxicity, and elastase and tyrosinase inhibitory activities of marigold (Tagetes erecta L.) flower extracts

    doi: 10.3892/etm.2013.1373

    Figure Lengend Snippet: Elastase inhibition activity of MF_EtOH (250 μg/ml) and MF_EtOAc (125 μg/ml) extracts compared with that of the negative control (n=3). a P

    Article Snippet: Ethanol (EtOH), ethyl acetate (EtOAc), dimethylsulfoxide (DMSO) and sodium dodecyl sulfate (SDS) were purchased from Merck KGaA (Darmstadt, Germany).

    Techniques: Inhibition, Activity Assay, Negative Control