Journal:

Article Title: In Vitro Activity of Daptomycin against Vancomycin-Resistant Enterococci of Various Van Types and Comparison of Susceptibility Testing Methods

doi: 10.1128/AAC.47.12.3760-3763.2003

Figure Lengend Snippet: Overall susceptibilities of the vancomycin-resistant Enterococcus species strain collection to daptomycin and the comparative agents by the NCCLS broth microdilution method using calcium-supplemented Mueller-Hinton broth test medium

Article Snippet: MICs were also determined for each isolate using specially prepared daptomycin E-test strips (prepared by AB Biodisk, Solna, Sweden, and provided by Cubist) with a concentration range of 0.016 to 256 μg/ml.

Techniques:

Journal:

Article Title: In Vitro Activity of Daptomycin against Vancomycin-Resistant Enterococci of Various Van Types and Comparison of Susceptibility Testing Methods

doi: 10.1128/AAC.47.12.3760-3763.2003

Figure Lengend Snippet: Comparison of daptomycin E test and NCCLS broth microdilution MICs according to the Mueller-Hinton agar test medium and E-test formulation tested

Article Snippet: MICs were also determined for each isolate using specially prepared daptomycin E-test strips (prepared by AB Biodisk, Solna, Sweden, and provided by Cubist) with a concentration range of 0.016 to 256 μg/ml.

Techniques: Comparison, Formulation

Journal:

Article Title: In Vitro Activity of Daptomycin against Vancomycin-Resistant Enterococci of Various Van Types and Comparison of Susceptibility Testing Methods

doi: 10.1128/AAC.47.12.3760-3763.2003

Figure Lengend Snippet: Comparison of daptomycin E test and NCCLS broth microdilution MICs utilizing a total of 116 isolates on a single brand and lot of Mueller-Hinton agar

Article Snippet: MICs were also determined for each isolate using specially prepared daptomycin E-test strips (prepared by AB Biodisk, Solna, Sweden, and provided by Cubist) with a concentration range of 0.016 to 256 μg/ml.

Techniques: Comparison

Journal:

Article Title: Genome-Wide Transcriptional Profiling of the Cyclic AMP-Dependent Signaling Pathway during Morphogenic Transitions of Candida albicans ▿ †

doi: 10.1128/EC.00318-07

Figure Lengend Snippet: Genes up-regulated by the cAMP signaling pathway in germ-tube-inducing conditions a

Article Snippet: Resuspended cells were evenly distributed onto solid RPMI 1640 medium (containing 2% glucose, morpholinepropanesulfonic acid [Sigma], 1.5% Bacto agar [Fisher Scientific]) by using a sterile swab and dried for at least 15 min. Fluconazole Etest strips (AB Biodisk) then were applied to the lawn of cells on the agar surface, and the plates were incubated at 35°C in a moist incubator until growth was clearly evident after 24 to 48 h. The MIC for each strain was determined using guidelines provided by the manufacturer.

Techniques: Binding Assay, Expressing

Journal:

Article Title: Genome-Wide Transcriptional Profiling of the Cyclic AMP-Dependent Signaling Pathway during Morphogenic Transitions of Candida albicans ▿ †

doi: 10.1128/EC.00318-07

Figure Lengend Snippet: Increased azole resistance by hyperactivation of the cAMP pathway of C. albicans. Susceptibilities to fluconazole were determined by the Etest strip method for the following isogenic strains: WT (UnoPP-1), pde2/pde2 (Δpde2; BPS15), pde2/PDE2 reintegrants (Δpde2+PDE2; BPS9), cap1/cap1 (Δcap1; CAC1-1A1E1), and cap1/CAP1 reintegrants (Δcap1+CAP1; CACRE1). The plates were photographed, and the fluconazole MICs for each strain were determined after 48 h of incubation at 35°C.

Article Snippet: Resuspended cells were evenly distributed onto solid RPMI 1640 medium (containing 2% glucose, morpholinepropanesulfonic acid [Sigma], 1.5% Bacto agar [Fisher Scientific]) by using a sterile swab and dried for at least 15 min. Fluconazole Etest strips (AB Biodisk) then were applied to the lawn of cells on the agar surface, and the plates were incubated at 35°C in a moist incubator until growth was clearly evident after 24 to 48 h. The MIC for each strain was determined using guidelines provided by the manufacturer.

Techniques: Stripping Membranes, Incubation

Journal:

Article Title: Rapid Detection of Point Mutations in the gyrA Gene of Helicobacter pylori Conferring Resistance to Ciprofloxacin by a Fluorescence Resonance Energy Transfer-Based Real-Time PCR Approach

doi: 10.1128/JCM.42.5.2241-2246.2004

Figure Lengend Snippet: Results of sequencing the QRDRs of 100 H. pylori isolates (65 Cip s and 35 Cip r ) and melting temperatures for each pair of hybridization probes (mutation probe 87 and mutation probe 91) depending on the genotype and phenotype

Article Snippet: We selected 65 ciprofloxacin-sensitive (Cip s ) and 35 Cip r H. pylori isolates and used the Etest method (AB Biodisk, Solna, Sweden) to determine the MIC of ciprofloxacin ( 4 ).

Techniques: Sequencing, Hybridization, Mutagenesis, Real-time Polymerase Chain Reaction