es-d3 Search Results


es d3  (ATCC)
96
ATCC es d3
Es D3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/ES-D3/pm14595122-30-10-14
Average 96 stars, based on 1 article reviews
es d3 - by Bioz Stars, 2026-09
96/100 stars
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pluripotent mouse embryonic stem es cells  (ATCC)
92
ATCC pluripotent mouse embryonic stem es cells
Pluripotent Mouse Embryonic Stem Es Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/Es-D3%3B+Embryonic+Stem+Cell%3B+Mouse/pmc01514553-39-5-14
Average 92 stars, based on 1 article reviews
pluripotent mouse embryonic stem es cells - by Bioz Stars, 2026-09
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d3 mes cells  (ATCC)
93
ATCC d3 mes cells
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
D3 Mes Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/ES-D3+(D3)%3B+Stem+Cell%3B+Mouse/pmc03333882-121-0-3
Average 93 stars, based on 1 article reviews
d3 mes cells - by Bioz Stars, 2026-09
93/100 stars
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embryonic stem cell culture undifferentiated mouse d3 es cells  (ATCC)
91
ATCC embryonic stem cell culture undifferentiated mouse d3 es cells
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Embryonic Stem Cell Culture Undifferentiated Mouse D3 Es Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/ES-D3%3B+Embryonic+Stem+Cell%3B+Mouse/pmc02802581-63-0-8
Average 91 stars, based on 1 article reviews
embryonic stem cell culture undifferentiated mouse d3 es cells - by Bioz Stars, 2026-09
91/100 stars
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mouse embryonic stem cell line esd3  (LGC Promochem)
90
LGC Promochem mouse embryonic stem cell line esd3
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Mouse Embryonic Stem Cell Line Esd3, supplied by LGC Promochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/mescs+esd3/pmc02995139-106-10-11
Average 90 stars, based on 1 article reviews
mouse embryonic stem cell line esd3 - by Bioz Stars, 2026-09
90/100 stars
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es (d3; incyte genomics, st. louis, mo) cells  (Incyte corporation)
90
Incyte corporation es (d3; incyte genomics, st. louis, mo) cells
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Es (D3; Incyte Genomics, St. Louis, Mo) Cells, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/es++d3++incyte+genomics++st++louis++mo++cells/pmc06063357-514-7-2
Average 90 stars, based on 1 article reviews
es (d3; incyte genomics, st. louis, mo) cells - by Bioz Stars, 2026-09
90/100 stars
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mes cells es-d3  (BioResource International Inc)
90
BioResource International Inc mes cells es-d3
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Mes Cells Es D3, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/murine+embryonic+pluripotent+stem+cells+es+d3/pmc04592426-101-3-9
Average 90 stars, based on 1 article reviews
mes cells es-d3 - by Bioz Stars, 2026-09
90/100 stars
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es-d3 cells  (Genome Systems Inc)
90
Genome Systems Inc es-d3 cells
Gli2 and MEF2C are expressed <t>during</t> <t>mES</t> differentiation. ( A ) Schematic representation of cardiomyogenesis in <t>D3</t> mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.
Es D3 Cells, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/es-d3/es+d3+cells/pm09828118-36-0-2
Average 90 stars, based on 1 article reviews
es-d3 cells - by Bioz Stars, 2026-09
90/100 stars
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es-d3 whole cell lysate  (Santa Cruz Biotechnology)
N/A
500 µg protein in 200 µl SDS-PAGE Western blotting buffer. mouse whole cell lysate; pluripotent embryonic stem cells. whole cell lysate provided as Western blotting positive control. should be stored at -20°C and repeated freezing
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es d3 whole cell lysate  (Novus Biologicals)
N/A
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Image Search Results


Gli2 and MEF2C are expressed during mES differentiation. ( A ) Schematic representation of cardiomyogenesis in D3 mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.

Journal: Nucleic Acids Research

Article Title: Gli2 and MEF2C activate each other's expression and function synergistically during cardiomyogenesis in vitro

doi: 10.1093/nar/gkr1232

Figure Lengend Snippet: Gli2 and MEF2C are expressed during mES differentiation. ( A ) Schematic representation of cardiomyogenesis in D3 mES cells, as described in ‘Materials and Methods’ section. ( B–E ) mES cells were differentiated and examined (B) for MHC expression using MF20 antibodies on Day 9 of differentiation. Nuclei were stained with Hoechst, scale bar is 30 μM; (C and D) by QPCR analysis for the indicated genes on Days 0, 3, 6 and 9 of differentiation, n = 3. Error bars represent ± SEM. Primers are listed in . (C) Values were compared with Day 0 values and statistical significance was determined using ANOVA (* P < 0.05 and ** P < 0.01). (D) To determine statistical significance between all days analysed, ANOVA followed by post hoc Tukey HSD analysis was performed, * P < 0.05 and ** P < 0.01; n.s. = not significant.; (E) immunoblot analysis of total protein extracts using MEF2C- and Gli2-specific antibodies at the time shown. Arrows designate Gli2 or MEF2C protein band(s), and asterisk denotes non-specific binding of the Gli2 antibodies. α-Tubulin served as a loading control. Lanes were spliced out from the same autoradiogram as designated by vertical lines. Gli2 band densities were measured from one representative experiment using ImageJ program , normalized to α-tubulin and expressed as percent maximum; ( F ) immunoblot analysis of eye total protein extracts from E16 Gli2 +/− and Gli2 −/− mice using Gli2-specific antibodies. Asterisk denotes non-specific binding of the antibodies. β-Actin served as a loading control.

Article Snippet: D3 mES cells (ATCC, # CRL-1934) were maintained as described in ref. ( , ).

Techniques: Expressing, Staining, Western Blot, Binding Assay, Control

Summary of gene expression changes in cell lines treated with or without DMSO

Journal: Nucleic Acids Research

Article Title: Gli2 and MEF2C activate each other's expression and function synergistically during cardiomyogenesis in vitro

doi: 10.1093/nar/gkr1232

Figure Lengend Snippet: Summary of gene expression changes in cell lines treated with or without DMSO

Article Snippet: D3 mES cells (ATCC, # CRL-1934) were maintained as described in ref. ( , ).

Techniques: Gene Expression