Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Erythrodiol chemical structure.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Hepatic histological analyses in male Apoe-deficient mice fed the different diets. Representative liver micrographs at 400× magnification from consuming a Western diet ( A ) and consuming a 10 mg/kg erythrodiol-containing Western diet ( B ). Liver sections (4 μm) from each mouse were stained with hematoxylin and eosin and blind evaluated. Bars denote 20 μm. Morphometric changes of hepatic fat surface in mice consuming the different diets ( C ) where data are means ± SD for each group ( n = 14 and n = 15, respectively for control and erythrodiol). Statistical analyses were done according to Mann–Whitney’s U-test.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Western Blot, Staining, Control

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Differentially expressed genes. ( A ), Venn diagram analysis. Control expressed 17,064 genes, while erythrodiol expressed 16,998 genes. Functional enrichment analysis of differentially expressed genes. ( B ), Gene ontology (GO) classification of biological processes of liver transcriptome by erythrodiol administration. X axis represents GO term. Y axis represents the amount of up/down-regulated genes. DEGs, differentially expressed genes.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Control, Functional Assay

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Significant expression changes and major networks involved. ( A ), Volcano plot representing control vs. erythrodiol differentially expressed genes; 68 up regulated genes and 124 down-regulated with false discovery rate q < 0.001; 16.375 no change with 2 fold change. ( B ), network of genes involved in detoxification, ( C ), network of genes involved in protein metabolism and ( D ), network associated with DNA. Red color denotes up-regulation while blue corresponds to down-regulation. ( E ), significant association of genes expressions assayed with RT-qPCR.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Expressing, Control, Quantitative RT-PCR

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Hepatic transcripts differentially up-regulated by the administration of erythrodiol at the level of signal log 2 ratio (SL 2 R) > 1.5 and false discovery rate < 0.001 in male Apoe-deficient mice according to RNAseq.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Cell Differentiation, Activity Assay, Selection, Ubiquitin Proteomics, Glycoproteomics, Binding Assay, Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Hepatic transcripts differentially down-regulated by the administration of erythrodiol at the level of signal log 2 ratio < 1.5 and false discovery rate < 0.001 in male Apoe -deficient mice according to RNAseq.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: RNA Binding Assay, Activity Assay, Cell Differentiation, Binding Assay, Coagulation, Protein Binding, Transmission Assay, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Changes in selected hepatic gene expressions of male Apoe -deficient mice receiving 10 mg/kg erythrodiol according to RT-qPCR assay.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Hepatic changes in selected gene expressions of female. Apoe -deficient mice receiving 10 mg/kg erythrodiol.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Hepatic changes in selected gene expressions of male Apoe -deficient mice receiving different doses of erythrodiol.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Dietary Erythrodiol Modifies Hepatic Transcriptome in Mice in a Sex and Dose-Dependent Way

doi: 10.3390/ijms21197331

Figure Lengend Snippet: Effect of 10 mg/kg erythrodiol on selected gene expressions in Apoa1 -deficient mice according to sex.

Article Snippet: Four study groups were established: female (12) and male control ( n = 14) groups received a purified Western diet containing 0.15% cholesterol and 20% refined palm oil (Gustav Heess, S.L., Barcelona, Spain), and the other two groups, female (13) and male ( n = 15), were fed with the same diet containing 0.01% erythrodiol (Extrasynthese, Genay, France).

Techniques: Control

Journal:

Article Title: Saponin Biosynthesis in Saponaria vaccaria . cDNAs Encoding β -Amyrin Synthase and a Triterpene Carboxylic Acid Glucosyltransferase 1 [OA]

doi: 10.1104/pp.106.088484

Figure Lengend Snippet: The substrate specificity of UGT74M1 UGT74M1 assays were performed as described in “Materials and Methods” using the radiochemical assay. The substrates for which no activity was detected are α -amyrin, β -amyrin, asiatic acid, benzoic acid, caffeic acid, cholesterol, cyanidin, diosgenin, erythrodiol, lupeol, oleanolic acid, quercetin, salicylic acid, and spinasterol.

Article Snippet: α -Amyrin, β -amyrin, echinocystic acid, erythrodiol, lupeol, oleanolic acid, and hederagenin were purchased from the Indofine Chemical Company.

Techniques: Radiochemical Assay, Activity Assay

Journal: Foods

Article Title: Phenolic Compounds and Triterpenes in Different Olive Tissues and Olive Oil By-Products, and Cytotoxicity on Human Colorectal Cancer Cells: The Case of Frantoio, Moraiolo and Leccino Cultivars ( Olea europaea L.)

doi: 10.3390/foods10112823

Figure Lengend Snippet: Distribution of maslinic acid, oleanolic acid, ursolic acid and erythrodiol in all the extracts from the three Tuscan varieties determined by HPLC-DAD. For each molecule, different letters indicate different contents among cultivars.

Article Snippet: Erythrodiol (55.4) , Frantoio , nd , 3219 a , nd , 976 b , nd , nd , 51.00 b.

Techniques:

Journal: Foods

Article Title: Phenolic Compounds and Triterpenes in Different Olive Tissues and Olive Oil By-Products, and Cytotoxicity on Human Colorectal Cancer Cells: The Case of Frantoio, Moraiolo and Leccino Cultivars ( Olea europaea L.)

doi: 10.3390/foods10112823

Figure Lengend Snippet: ( A ) IC 50 values of the phenolic and triterpenoid extracts from Frantoio cv; ( B ) IC 50 values of some representative pure phenols and triterpenoids.

Article Snippet: Erythrodiol (55.4) , Frantoio , nd , 3219 a , nd , 976 b , nd , nd , 51.00 b.

Techniques:

Journal:

Article Title: Saponin Biosynthesis in Saponaria vaccaria . cDNAs Encoding β -Amyrin Synthase and a Triterpene Carboxylic Acid Glucosyltransferase 1 [OA]

doi: 10.1104/pp.106.088484

Figure Lengend Snippet: The substrate specificity of UGT74M1 UGT74M1 assays were performed as described in “Materials and Methods” using the radiochemical assay. The substrates for which no activity was detected are α -amyrin, β -amyrin, asiatic acid, benzoic acid, caffeic acid, cholesterol, cyanidin, diosgenin, erythrodiol, lupeol, oleanolic acid, quercetin, salicylic acid, and spinasterol.

Article Snippet: Chemicals α -Amyrin, β -amyrin, echinocystic acid, erythrodiol, lupeol, oleanolic acid, and hederagenin were purchased from the Indofine Chemical Company.

Techniques: Radiochemical Assay, Activity Assay