ermb Search Results


gene exp ermb pa04230913 s1  (Thermo Fisher)
93
Thermo Fisher gene exp ermb pa04230913 s1
Gene Exp Ermb Pa04230913 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hmra::tn(ermb) jmb 7270  (BEI Resources)
90
BEI Resources hmra::tn(ermb) jmb 7270
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Hmra/Tn(Ermb) Jmb 7270, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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erythromycin cassette ermb  (Promega)
90
Promega erythromycin cassette ermb
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Erythromycin Cassette Ermb, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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saes ::tn ( ermb ) lac  (BEI Resources)
90
BEI Resources saes ::tn ( ermb ) lac
Increased amounts of SaeR∼P are required for fermentative biofilm formation, and the <t>SaeS</t> kinase activity inhibitor zinc attenuates fermentative biofilm formation. (A) Transcript levels corresponding to coa are decreased in a ΔsaePQRS strain during fermentative growth. Biofilms of the <t>WT</t> <t>(JMB</t> 1100) and ΔsaePQRS::spc (JMB 1335) strains were cultured fermentatively, mRNA was extracted, and the abundance of the coa transcript was quantified. Data were normalized to 16S rRNA levels and thereafter to the levels observed in the WT. (B) The Newman strain of S. aureus forms SaeSP18-dependent biofilms during aerobic growth. Aerobic biofilm formation for the Newman strains containing the wild-type saeSP18 allele (JMB 1422), the saeSL18 allele (JMB 8263), or the saeS::Tn (JMB 7077) mutation is displayed. (C) Constitutive expression of the saeSP18 allele induces aerobic biofilm formation in the LAC WT strain. The biofilm formation of the LAC WT strain (JMB 1100) carrying pOS (pEV) or pOS_saeSP18R (psaeSP18R) following aerobic growth is displayed. (D) Supplementation of the growth medium with zinc, an inhibitor of SaeS kinase activity, results in a concentration-dependent decrease in fermentative biofilm formation. The biofilm formation of the LAC WT and the LAC saeS::Tn (JMB 7076) strains following fermentative growth in medium supplemented with various concentrations of zinc is displayed. The data represent the average values for eight wells (B to D) or from triplicate assays (A), and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.
Saes /Tn ( Ermb ) Lac, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ermb-specific probe labeled digoxigenin  (Boehringer Mannheim)
90
Boehringer Mannheim ermb-specific probe labeled digoxigenin
Increased amounts of SaeR∼P are required for fermentative biofilm formation, and the <t>SaeS</t> kinase activity inhibitor zinc attenuates fermentative biofilm formation. (A) Transcript levels corresponding to coa are decreased in a ΔsaePQRS strain during fermentative growth. Biofilms of the <t>WT</t> <t>(JMB</t> 1100) and ΔsaePQRS::spc (JMB 1335) strains were cultured fermentatively, mRNA was extracted, and the abundance of the coa transcript was quantified. Data were normalized to 16S rRNA levels and thereafter to the levels observed in the WT. (B) The Newman strain of S. aureus forms SaeSP18-dependent biofilms during aerobic growth. Aerobic biofilm formation for the Newman strains containing the wild-type saeSP18 allele (JMB 1422), the saeSL18 allele (JMB 8263), or the saeS::Tn (JMB 7077) mutation is displayed. (C) Constitutive expression of the saeSP18 allele induces aerobic biofilm formation in the LAC WT strain. The biofilm formation of the LAC WT strain (JMB 1100) carrying pOS (pEV) or pOS_saeSP18R (psaeSP18R) following aerobic growth is displayed. (D) Supplementation of the growth medium with zinc, an inhibitor of SaeS kinase activity, results in a concentration-dependent decrease in fermentative biofilm formation. The biofilm formation of the LAC WT and the LAC saeS::Tn (JMB 7076) strains following fermentative growth in medium supplemented with various concentrations of zinc is displayed. The data represent the average values for eight wells (B to D) or from triplicate assays (A), and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.
Ermb Specific Probe Labeled Digoxigenin, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ermb-specific probe labeled digoxigenin/product/Boehringer Mannheim
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icaa::tn(ermb  (BEI Resources)
90
BEI Resources icaa::tn(ermb
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Icaa/Tn(Ermb, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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synthetic dna fragments of different ermbl-ermb variants  (Twist Bioscience)
90
Twist Bioscience synthetic dna fragments of different ermbl-ermb variants
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Synthetic Dna Fragments Of Different Ermbl Ermb Variants, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primers for sul 1  (Metabion International AG)
90
Metabion International AG primers for sul 1
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Primers For Sul 1, supplied by Metabion International AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s. pyogenes 6945 (ermb)  (Abbott Laboratories)
90
Abbott Laboratories s. pyogenes 6945 (ermb)
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
S. Pyogenes 6945 (Ermb), supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ermb gene  (Mayrhofer Pharmazeutika)
90
Mayrhofer Pharmazeutika ermb gene
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Ermb Gene, supplied by Mayrhofer Pharmazeutika, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ermb gene - by Bioz Stars, 2026-04
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oligonucleotide primers for erma, ermb, ermc, msra/ msrb, erea and ereb  (Pfizer Inc)
90
Pfizer Inc oligonucleotide primers for erma, ermb, ermc, msra/ msrb, erea and ereb
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Oligonucleotide Primers For Erma, Ermb, Ermc, Msra/ Msrb, Erea And Ereb, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oligonucleotide primers for erma, ermb, ermc, msra/ msrb, erea and ereb/product/Pfizer Inc
Average 90 stars, based on 1 article reviews
oligonucleotide primers for erma, ermb, ermc, msra/ msrb, erea and ereb - by Bioz Stars, 2026-04
90/100 stars
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gene exp ermb ba04230913 s1  (Thermo Fisher)
88
Thermo Fisher gene exp ermb ba04230913 s1
Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018
Gene Exp Ermb Ba04230913 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp ermb ba04230913 s1/product/Thermo Fisher
Average 88 stars, based on 1 article reviews
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Image Search Results


Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018

Journal: eLife

Article Title: Impaired respiration elicits SrrAB-dependent programmed cell lysis and biofilm formation in Staphylococcus aureus

doi: 10.7554/eLife.23845

Figure Lengend Snippet: Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018

Article Snippet: JMB 7270 , hmrA::Tn(ermB) , JE2 , BEI resources and .

Techniques: Control, Plasmid Preparation, Cloning

Increased amounts of SaeR∼P are required for fermentative biofilm formation, and the SaeS kinase activity inhibitor zinc attenuates fermentative biofilm formation. (A) Transcript levels corresponding to coa are decreased in a ΔsaePQRS strain during fermentative growth. Biofilms of the WT (JMB 1100) and ΔsaePQRS::spc (JMB 1335) strains were cultured fermentatively, mRNA was extracted, and the abundance of the coa transcript was quantified. Data were normalized to 16S rRNA levels and thereafter to the levels observed in the WT. (B) The Newman strain of S. aureus forms SaeSP18-dependent biofilms during aerobic growth. Aerobic biofilm formation for the Newman strains containing the wild-type saeSP18 allele (JMB 1422), the saeSL18 allele (JMB 8263), or the saeS::Tn (JMB 7077) mutation is displayed. (C) Constitutive expression of the saeSP18 allele induces aerobic biofilm formation in the LAC WT strain. The biofilm formation of the LAC WT strain (JMB 1100) carrying pOS (pEV) or pOS_saeSP18R (psaeSP18R) following aerobic growth is displayed. (D) Supplementation of the growth medium with zinc, an inhibitor of SaeS kinase activity, results in a concentration-dependent decrease in fermentative biofilm formation. The biofilm formation of the LAC WT and the LAC saeS::Tn (JMB 7076) strains following fermentative growth in medium supplemented with various concentrations of zinc is displayed. The data represent the average values for eight wells (B to D) or from triplicate assays (A), and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.

Journal: Infection and Immunity

Article Title: SaeRS Is Responsive to Cellular Respiratory Status and Regulates Fermentative Biofilm Formation in Staphylococcus aureus

doi: 10.1128/IAI.00157-17

Figure Lengend Snippet: Increased amounts of SaeR∼P are required for fermentative biofilm formation, and the SaeS kinase activity inhibitor zinc attenuates fermentative biofilm formation. (A) Transcript levels corresponding to coa are decreased in a ΔsaePQRS strain during fermentative growth. Biofilms of the WT (JMB 1100) and ΔsaePQRS::spc (JMB 1335) strains were cultured fermentatively, mRNA was extracted, and the abundance of the coa transcript was quantified. Data were normalized to 16S rRNA levels and thereafter to the levels observed in the WT. (B) The Newman strain of S. aureus forms SaeSP18-dependent biofilms during aerobic growth. Aerobic biofilm formation for the Newman strains containing the wild-type saeSP18 allele (JMB 1422), the saeSL18 allele (JMB 8263), or the saeS::Tn (JMB 7077) mutation is displayed. (C) Constitutive expression of the saeSP18 allele induces aerobic biofilm formation in the LAC WT strain. The biofilm formation of the LAC WT strain (JMB 1100) carrying pOS (pEV) or pOS_saeSP18R (psaeSP18R) following aerobic growth is displayed. (D) Supplementation of the growth medium with zinc, an inhibitor of SaeS kinase activity, results in a concentration-dependent decrease in fermentative biofilm formation. The biofilm formation of the LAC WT and the LAC saeS::Tn (JMB 7076) strains following fermentative growth in medium supplemented with various concentrations of zinc is displayed. The data represent the average values for eight wells (B to D) or from triplicate assays (A), and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.

Article Snippet: JMB 7076 , saeS ::Tn ( ermB ) , LAC , This work, BEI Resources, and reference 75.

Techniques: Activity Assay, Cell Culture, Mutagenesis, Expressing, Concentration Assay, Two Tailed Test

SaeRS-dependent biofilm formation is responsive to the cellular respiratory status, and a menaquinone auxotroph forms Sae-dependent biofilms. (A) The biofilm formation of a ΔsaePQRS strain is attenuated to a lower degree upon supplementation of anaerobic biofilms with the alternate electron acceptor nitrate. The biofilm formation of the WT (JMB 1100) and the ΔsaePQRS::spc (JMB 1335) strains following anaerobic growth in the presence or absence of 1 mM nitrate is displayed. (B) Induction of biofilm formation in a ΔsaePQRS strain is attenuated upon chemical inhibition of respiration. The biofilm formation of the WT and ΔsaePQRS::spc strains following aerobic growth in the presence or absence of 50 μM azide is displayed. (C) A menaquinone auxotroph forms SaeRS-dependent biofilms aerobically. The aerobic biofilm formation of the WT, menF::Tn (JMB 6219), saeR::Tn menF::Tn (JMB 7109), and saeS::Tn menF::Tn (JMB 7081) strains is displayed. The data represent the average values for eight wells, and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.

Journal: Infection and Immunity

Article Title: SaeRS Is Responsive to Cellular Respiratory Status and Regulates Fermentative Biofilm Formation in Staphylococcus aureus

doi: 10.1128/IAI.00157-17

Figure Lengend Snippet: SaeRS-dependent biofilm formation is responsive to the cellular respiratory status, and a menaquinone auxotroph forms Sae-dependent biofilms. (A) The biofilm formation of a ΔsaePQRS strain is attenuated to a lower degree upon supplementation of anaerobic biofilms with the alternate electron acceptor nitrate. The biofilm formation of the WT (JMB 1100) and the ΔsaePQRS::spc (JMB 1335) strains following anaerobic growth in the presence or absence of 1 mM nitrate is displayed. (B) Induction of biofilm formation in a ΔsaePQRS strain is attenuated upon chemical inhibition of respiration. The biofilm formation of the WT and ΔsaePQRS::spc strains following aerobic growth in the presence or absence of 50 μM azide is displayed. (C) A menaquinone auxotroph forms SaeRS-dependent biofilms aerobically. The aerobic biofilm formation of the WT, menF::Tn (JMB 6219), saeR::Tn menF::Tn (JMB 7109), and saeS::Tn menF::Tn (JMB 7081) strains is displayed. The data represent the average values for eight wells, and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.

Article Snippet: JMB 7076 , saeS ::Tn ( ermB ) , LAC , This work, BEI Resources, and reference 75.

Techniques: Inhibition, Two Tailed Test

SaeRS and SrrAB influence fermentative biofilm formation independently of one another. (A) Induction of fermentative biofilm formation is nearly absent in the ΔsrrAB saeS::Tn strain. The biofilm formation of the WT (JMB 1100) and the ΔsrrAB saeS::Tn (JMB 7068) strains following aerobic and fermentative growth is displayed. To allow assessment of induction levels, the data were normalized to the biofilm levels displayed by each strain under aerobic growth. (B and C) The biofilm formation phenotypes of the ΔsaeS::Tn and ΔsrrAB mutations are additive. The biofilm formation of the WT, ΔsrrAB (JMB 1467), saeS::Tn (JMB 7076), and ΔsrrAB saeS::Tn strains following fermentative growth is displayed. Data were normalized with respect to the WT (B) or with respect to the parental strain into which the ΔsrrAB mutation was introduced (i.e., the WT or saeS::Tn strain) (C). The data represent the average values for eight wells, and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.

Journal: Infection and Immunity

Article Title: SaeRS Is Responsive to Cellular Respiratory Status and Regulates Fermentative Biofilm Formation in Staphylococcus aureus

doi: 10.1128/IAI.00157-17

Figure Lengend Snippet: SaeRS and SrrAB influence fermentative biofilm formation independently of one another. (A) Induction of fermentative biofilm formation is nearly absent in the ΔsrrAB saeS::Tn strain. The biofilm formation of the WT (JMB 1100) and the ΔsrrAB saeS::Tn (JMB 7068) strains following aerobic and fermentative growth is displayed. To allow assessment of induction levels, the data were normalized to the biofilm levels displayed by each strain under aerobic growth. (B and C) The biofilm formation phenotypes of the ΔsaeS::Tn and ΔsrrAB mutations are additive. The biofilm formation of the WT, ΔsrrAB (JMB 1467), saeS::Tn (JMB 7076), and ΔsrrAB saeS::Tn strains following fermentative growth is displayed. Data were normalized with respect to the WT (B) or with respect to the parental strain into which the ΔsrrAB mutation was introduced (i.e., the WT or saeS::Tn strain) (C). The data represent the average values for eight wells, and error bars represent standard deviations. Error bars are displayed for all data but on occasion may be too small to see. Statistical significance was calculated using a two-tailed Student's t test. *, P < 0.05.

Article Snippet: JMB 7076 , saeS ::Tn ( ermB ) , LAC , This work, BEI Resources, and reference 75.

Techniques: Mutagenesis, Two Tailed Test

Microbial strains used in this study

Journal: Infection and Immunity

Article Title: SaeRS Is Responsive to Cellular Respiratory Status and Regulates Fermentative Biofilm Formation in Staphylococcus aureus

doi: 10.1128/IAI.00157-17

Figure Lengend Snippet: Microbial strains used in this study

Article Snippet: JMB 7076 , saeS ::Tn ( ermB ) , LAC , This work, BEI Resources, and reference 75.

Techniques:

Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018

Journal: eLife

Article Title: Impaired respiration elicits SrrAB-dependent programmed cell lysis and biofilm formation in Staphylococcus aureus

doi: 10.7554/eLife.23845

Figure Lengend Snippet: Strains and plasmids used in this study. DOI: http://dx.doi.org/10.7554/eLife.23845.018

Article Snippet: JMB 5577 , icaA::Tn(ermB) , LAC , This work; BEI resources and .

Techniques: Plasmid Preparation, Clone Assay