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Image Search Results
Journal: Cell Death & Disease
Article Title: A novel bystander effect in tamoxifen treatment: PPIB derived from ER+ cells attenuates ER− cells via endoplasmic reticulum stress-induced apoptosis
doi: 10.1038/s41419-024-06539-3
Figure Lengend Snippet: CM conditioned medium, CN control medium, CO co-culture group, CT control group. *** p value < 0.001, ns p value > 0.05. n = 3. A – C Enhanced endoplasmic reticulum tracker MFI in MDA-MB-231 and BT-549 cells co-cultured with MCF7 cells. D Upregulated ERS markers and enhanced apoptosis in MDA-MB-231 and BT-549 cells co-cultured with MCF7 cells and treated with CM. ERS markers and apoptotic markers were suppressed by 4-PBA in MDA-MB-231 and BT-549 cells. E Enhanced fluorescence of CHOP detected by immunofluorescence in MDA-MB-231 and BT-549 cells co-cultured with MCF7 cells. F – J Increased proportions of apoptotic MDA-MB-231 and BT-549 cells co-cultured with MCF7 cells and treated with CM, and no statistical increase in apoptotic proportions was detected in 4-PBA-treated ER− cells. K Increased TUNEL-positive stained cells in CM-injected MDA-MB-231 xenografts.
Article Snippet: To label the endoplasmic reticulum, cells were incubated with 1 μM
Techniques: Control, Co-Culture Assay, Cell Culture, Fluorescence, Immunofluorescence, TUNEL Assay, Staining, Injection
Journal: Cell Death & Disease
Article Title: A novel bystander effect in tamoxifen treatment: PPIB derived from ER+ cells attenuates ER− cells via endoplasmic reticulum stress-induced apoptosis
doi: 10.1038/s41419-024-06539-3
Figure Lengend Snippet: CO co-culture group, CT control group. *** p value < 0.001, ns p value > 0.05. n = 3. A – C Enhanced endoplasmic reticulum tracker MFI in PPIB-treated MDA-MB-231 and BT-549 cells. D Upregulated ERS markers and apoptotic markers in PPIB-treated MDA-MB-231 and BT-549 cells, and ERS-induced apoptosis can be suppressed by ERS inhibitor 4-PBA. E – G Increased proportions of apoptotic MDA-MB-231 and BT-549 cells treated with PPIB, and no increase in apoptosis in cells treated with 4-PBA. H Increased TUNEL-positive stained cells in PPIB-injected MDA-MB-231 xenografts. I Enhanced GDF15 expression in PPIB-injected MDA-MB-231 xenografts. J Upregulated ERS markers and GDF15 were not detected in MDA-MB-231 and BT-549 cells co-cultured with PPIB-knockdown MCF7 cells while reintroducing PPIB into the medium re-activated TAM-induced bystander effect.
Article Snippet: To label the endoplasmic reticulum, cells were incubated with 1 μM
Techniques: Co-Culture Assay, Control, TUNEL Assay, Staining, Injection, Expressing, Cell Culture, Knockdown