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  • 93
    Carl Zeiss epifluorescence
    Apoptosis during OHSC cultivation . OHSC from POMC-eGFP mice were cultivated for 1, 3, or 7 DIV, fixed, and stained as whole slices with an antibody against activated caspase-3 (Act. Casp-3). (A–O) Photomicrographs, obtained by an <t>epifluorescence</t> microscope, reveal many apoptotic cells in all neuronal cell layers except for the GCL during the first days of cultivation. (C–E,H–J,M–O) High power views of the DG, marked by white frames in (A,F,K) . At 1 DIV, the extent of apoptosis is highest (A–E) , followed by a subsequent decline at 3 DIV (F–J) and at 7 DIV (K–O) . Scale bar: 500 μm (whole slice images: A,F,K ) and 50 μm (magnifications: C,H,M ). Please note that the eGFP photomicrographs were taken with increasing exposure times (1 DIV: 950 ms, 3 DIV: 1600 ms, 7 DIV: 5000 ms). (P–R) Confocal mircoscopical analysis confirms the low apoptotic rates of granule cell progenitors at 1, 3, and 7 DIV. At 1 and 3 DIV, when high rates of cell death are visible, most apoptotic cells within the DG are eGFP-negative. Images are Z projections of 10 stacks (1 μm step size). Scale bar: 50 μm.
    Epifluorescence, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 4631 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Leica Microsystems epifluorescence
    Effect of Hg exposure on acidic compartmentalization of A . parkinsoniana labeled with AO. <t>Epifluorescence</t> micrographs of single optical sections showing overlay of AO green and red fluorescence for (A) T1-control, (B,C) T1-100 ppm, and (D) T2-100 ppm, Bars: 20μm. (E) Histogram of maximum dimension (diameter) of acidic vesicles. (F) Histogram of red Mean Fluorescence Intensity (MFI) expressed in arbitrary units (A.U.) for control and 100 ppm at both T1 and T2. Error bars indicate ± standard error of the mean.
    Epifluorescence, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 92/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Nikon epifluorescence
    SCNT embryos-derived exosomes were identified by immunofluorescence. The exosomes were bound to beads of a size that was in the detection range of the fluorescence microscope (4-μm diameter latex beads). The beads were then bound to fluorescence-conjugated antibody against CD9. Images were taken under <t>epifluorescence</t> (A, C, E) and DIC (B, D, F). A,B: embryos-derived exosomes; C,D: IgG negative control; E,F: embryo-free culture medium control. Bar, 10 μm.
    Epifluorescence, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 3256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Olympus epifluorescence
    Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted <t>epifluorescence</t> microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).
    Epifluorescence, supplied by Olympus, used in various techniques. Bioz Stars score: 94/100, based on 3029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher epifluorescence
    Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted <t>epifluorescence</t> microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).
    Epifluorescence, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Chroma Technology Corporation epifluorescence
    Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted <t>epifluorescence</t> microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).
    Epifluorescence, supplied by Chroma Technology Corporation, used in various techniques. Bioz Stars score: 92/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Diagnostic Instruments Inc epifluorescence
    Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted <t>epifluorescence</t> microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).
    Epifluorescence, supplied by Diagnostic Instruments Inc, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Collaborative Drug Discovery Inc epifluorescence
    Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted <t>epifluorescence</t> microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).
    Epifluorescence, supplied by Collaborative Drug Discovery Inc, used in various techniques. Bioz Stars score: 93/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Carl Zeiss epifluorescent
    Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted <t>epifluorescence</t> microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).
    Epifluorescent, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 89/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    KEYENCE epifluorescence
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence, supplied by KEYENCE, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Scientifica epifluorescence
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence, supplied by Scientifica, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Leica Microsystems epifluorescent
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescent, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 91/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Olympus coaxial epifluorescence
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Coaxial Epifluorescence, supplied by Olympus, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Olympus epifluorescence filter
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence Filter, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Carl Zeiss epifluorescence macroscope
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence Macroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Nikon epifluorescence macroscope
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence Macroscope, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Leica Microsystems epifluorescence microcopy
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence Microcopy, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Inscopix epifluorescence microscope
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence Microscope, supplied by Inscopix, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Leica Biosystems epifluorescence microscope
    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an <t>epifluorescence</t> microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p
    Epifluorescence Microscope, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Apoptosis during OHSC cultivation . OHSC from POMC-eGFP mice were cultivated for 1, 3, or 7 DIV, fixed, and stained as whole slices with an antibody against activated caspase-3 (Act. Casp-3). (A–O) Photomicrographs, obtained by an epifluorescence microscope, reveal many apoptotic cells in all neuronal cell layers except for the GCL during the first days of cultivation. (C–E,H–J,M–O) High power views of the DG, marked by white frames in (A,F,K) . At 1 DIV, the extent of apoptosis is highest (A–E) , followed by a subsequent decline at 3 DIV (F–J) and at 7 DIV (K–O) . Scale bar: 500 μm (whole slice images: A,F,K ) and 50 μm (magnifications: C,H,M ). Please note that the eGFP photomicrographs were taken with increasing exposure times (1 DIV: 950 ms, 3 DIV: 1600 ms, 7 DIV: 5000 ms). (P–R) Confocal mircoscopical analysis confirms the low apoptotic rates of granule cell progenitors at 1, 3, and 7 DIV. At 1 and 3 DIV, when high rates of cell death are visible, most apoptotic cells within the DG are eGFP-negative. Images are Z projections of 10 stacks (1 μm step size). Scale bar: 50 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Persistent Gliosis Interferes with Neurogenesis in Organotypic Hippocampal Slice Cultures

    doi: 10.3389/fncel.2016.00131

    Figure Lengend Snippet: Apoptosis during OHSC cultivation . OHSC from POMC-eGFP mice were cultivated for 1, 3, or 7 DIV, fixed, and stained as whole slices with an antibody against activated caspase-3 (Act. Casp-3). (A–O) Photomicrographs, obtained by an epifluorescence microscope, reveal many apoptotic cells in all neuronal cell layers except for the GCL during the first days of cultivation. (C–E,H–J,M–O) High power views of the DG, marked by white frames in (A,F,K) . At 1 DIV, the extent of apoptosis is highest (A–E) , followed by a subsequent decline at 3 DIV (F–J) and at 7 DIV (K–O) . Scale bar: 500 μm (whole slice images: A,F,K ) and 50 μm (magnifications: C,H,M ). Please note that the eGFP photomicrographs were taken with increasing exposure times (1 DIV: 950 ms, 3 DIV: 1600 ms, 7 DIV: 5000 ms). (P–R) Confocal mircoscopical analysis confirms the low apoptotic rates of granule cell progenitors at 1, 3, and 7 DIV. At 1 and 3 DIV, when high rates of cell death are visible, most apoptotic cells within the DG are eGFP-negative. Images are Z projections of 10 stacks (1 μm step size). Scale bar: 50 μm.

    Article Snippet: Sections were counterstained with DAPI, coverslipped in fluorescence mounting medium (Dako) and analyzed using an epifluorescence (Axio Imager 2, Carl Zeiss) or confocal microscope (Olympus FluoView FV10i).

    Techniques: Mouse Assay, Staining, Activated Clotting Time Assay, Microscopy, Mass Spectrometry

    Impact of RAGE signaling on cell mechanical properties. A, Representative epifluorescence micrographs (40X) of Phalloidine/TexasRED stained cells that exhibit actin organization, (B) histogram of the average TexasRED fluorescence that corresponds to the actin density, (C) histogram indicates cell rigidity (Young Modulus) and (D) cell heights measured by AFM of A7r5 cells stimulated for 24 hours with PBS, 1 mg/ml AGE-HSA or 1 mg/ml CML-HSA. Each bar represents 12–15 independent experiments. * p

    Journal: PLoS ONE

    Article Title: Receptor for Advanced Glycation End-Products Signaling Interferes with the Vascular Smooth Muscle Cell Contractile Phenotype and Function

    doi: 10.1371/journal.pone.0128881

    Figure Lengend Snippet: Impact of RAGE signaling on cell mechanical properties. A, Representative epifluorescence micrographs (40X) of Phalloidine/TexasRED stained cells that exhibit actin organization, (B) histogram of the average TexasRED fluorescence that corresponds to the actin density, (C) histogram indicates cell rigidity (Young Modulus) and (D) cell heights measured by AFM of A7r5 cells stimulated for 24 hours with PBS, 1 mg/ml AGE-HSA or 1 mg/ml CML-HSA. Each bar represents 12–15 independent experiments. * p

    Article Snippet: An inverted epifluorescence microscope (Axio Observer Z1, Carl Zeiss) equipped with an AxioCam MRm camera (Carl Zeiss) was used for fluorescence acquisition.

    Techniques: Staining, Fluorescence

    RAGE stimulation increases NF-κB activity. A, Representative epifluorescence micrographs (20X) and (B) Histogram of the average GFP fluorescence intensities indicate NF-κB dependent GFP expression in A7r5 cells stimulated for 24 hours with PBS, 1 mg/ml AGE-HSA or 1 mg/ml CML-HSA. Each bar represents 10–12 independent experiments. * p

    Journal: PLoS ONE

    Article Title: Receptor for Advanced Glycation End-Products Signaling Interferes with the Vascular Smooth Muscle Cell Contractile Phenotype and Function

    doi: 10.1371/journal.pone.0128881

    Figure Lengend Snippet: RAGE stimulation increases NF-κB activity. A, Representative epifluorescence micrographs (20X) and (B) Histogram of the average GFP fluorescence intensities indicate NF-κB dependent GFP expression in A7r5 cells stimulated for 24 hours with PBS, 1 mg/ml AGE-HSA or 1 mg/ml CML-HSA. Each bar represents 10–12 independent experiments. * p

    Article Snippet: An inverted epifluorescence microscope (Axio Observer Z1, Carl Zeiss) equipped with an AxioCam MRm camera (Carl Zeiss) was used for fluorescence acquisition.

    Techniques: Activity Assay, Fluorescence, Expressing

    Effect of Hg exposure on acidic compartmentalization of A . parkinsoniana labeled with AO. Epifluorescence micrographs of single optical sections showing overlay of AO green and red fluorescence for (A) T1-control, (B,C) T1-100 ppm, and (D) T2-100 ppm, Bars: 20μm. (E) Histogram of maximum dimension (diameter) of acidic vesicles. (F) Histogram of red Mean Fluorescence Intensity (MFI) expressed in arbitrary units (A.U.) for control and 100 ppm at both T1 and T2. Error bars indicate ± standard error of the mean.

    Journal: PLoS ONE

    Article Title: Mercury-Pollution Induction of Intracellular Lipid Accumulation and Lysosomal Compartment Amplification in the Benthic Foraminifer Ammonia parkinsoniana

    doi: 10.1371/journal.pone.0162401

    Figure Lengend Snippet: Effect of Hg exposure on acidic compartmentalization of A . parkinsoniana labeled with AO. Epifluorescence micrographs of single optical sections showing overlay of AO green and red fluorescence for (A) T1-control, (B,C) T1-100 ppm, and (D) T2-100 ppm, Bars: 20μm. (E) Histogram of maximum dimension (diameter) of acidic vesicles. (F) Histogram of red Mean Fluorescence Intensity (MFI) expressed in arbitrary units (A.U.) for control and 100 ppm at both T1 and T2. Error bars indicate ± standard error of the mean.

    Article Snippet: Epifluorescence and bright field (BF) microscopies were performed using a CLSM (Leica TCS SP5 II confocal microscope, Leica Microsystems) with 488-, 543- and 633-nm illumination and oil-immersion objectives.

    Techniques: Labeling, Fluorescence

    Citrullination of collagen I alters adhesion, motility and epithelial mesenchymal plasticity of CRC cells. a Recombinant collagen type I alone, collagen type I pre-treated with recombinant PAD4, or collagen I pre-treated with recombinant PAD4 and BB-Cl-amidine, were incubated and subjected to LC-MS/MS analysis. Shown are the numbers of citrullinated peptides. Repeated twice. b GFP + CRC cells were plated in wells pre-coated with either collagen type I alone, collagen type I pre-treated with recombinant PAD4 or collagen type I pre-treated with PAD4 and BB-Cl-amidine. Cells were imaged at the indicated time points using an epifluorescence microscope. Four to five fields of view per condition were taken. Shown are the cell numbers attached to the plate for each condition. c GFP + MC38 cells were plated in wells pre-coated with either collagen type I alone, collagen type I pre-treated with recombinant PAD4, or collagen type I pre-treated with addition of PAD4 and BB-Cl-amidine. Individual cells were tracked using time-lapse microscopy. Shown is the quantification of cell median length and speed. d Representative images of cell tracks from the experiment in ( c ). Scale bar = 200 μm. e Cancer cells were plated in wells pre-coated with either collagen type I alone or collagen type I pre-treated with recombinant PAD4. At indicated times cells were collected and probed for the indicated proteins. Numbers indicate relative expression. f Shown is mRNA expression of different EMT genes in MC38 cells seeded on collagen type I pre-treated with PAD4 compared to those seeded on unmodified collagen type I 18 h post-seeding. Shown is average of 2 technical replicates. g Immunoblotting for the indicated proteins performed on cell lysates collected from the experiment in ( f ). GAPDH was used as a loading control. For a and b , error bars indicate s.e.m. center values indicate mean (* P

    Journal: Nature Communications

    Article Title: Colorectal cancer liver metastatic growth depends on PAD4-driven citrullination of the extracellular matrix

    doi: 10.1038/s41467-018-07306-7

    Figure Lengend Snippet: Citrullination of collagen I alters adhesion, motility and epithelial mesenchymal plasticity of CRC cells. a Recombinant collagen type I alone, collagen type I pre-treated with recombinant PAD4, or collagen I pre-treated with recombinant PAD4 and BB-Cl-amidine, were incubated and subjected to LC-MS/MS analysis. Shown are the numbers of citrullinated peptides. Repeated twice. b GFP + CRC cells were plated in wells pre-coated with either collagen type I alone, collagen type I pre-treated with recombinant PAD4 or collagen type I pre-treated with PAD4 and BB-Cl-amidine. Cells were imaged at the indicated time points using an epifluorescence microscope. Four to five fields of view per condition were taken. Shown are the cell numbers attached to the plate for each condition. c GFP + MC38 cells were plated in wells pre-coated with either collagen type I alone, collagen type I pre-treated with recombinant PAD4, or collagen type I pre-treated with addition of PAD4 and BB-Cl-amidine. Individual cells were tracked using time-lapse microscopy. Shown is the quantification of cell median length and speed. d Representative images of cell tracks from the experiment in ( c ). Scale bar = 200 μm. e Cancer cells were plated in wells pre-coated with either collagen type I alone or collagen type I pre-treated with recombinant PAD4. At indicated times cells were collected and probed for the indicated proteins. Numbers indicate relative expression. f Shown is mRNA expression of different EMT genes in MC38 cells seeded on collagen type I pre-treated with PAD4 compared to those seeded on unmodified collagen type I 18 h post-seeding. Shown is average of 2 technical replicates. g Immunoblotting for the indicated proteins performed on cell lysates collected from the experiment in ( f ). GAPDH was used as a loading control. For a and b , error bars indicate s.e.m. center values indicate mean (* P

    Article Snippet: At 1, 2, and 3 h time points, the plates were imaged using an inverted epifluorescence microscope at ×10 objective (DM IRBE, Leica Microsystems, at least five fields of view per condition) and the number of adherent cells was manually counted.

    Techniques: Recombinant, Incubation, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry, Microscopy, Time-lapse Microscopy, Expressing

    SCNT embryos-derived exosomes were identified by immunofluorescence. The exosomes were bound to beads of a size that was in the detection range of the fluorescence microscope (4-μm diameter latex beads). The beads were then bound to fluorescence-conjugated antibody against CD9. Images were taken under epifluorescence (A, C, E) and DIC (B, D, F). A,B: embryos-derived exosomes; C,D: IgG negative control; E,F: embryo-free culture medium control. Bar, 10 μm.

    Journal: PLoS ONE

    Article Title: Effects of embryo-derived exosomes on the development of bovine cloned embryos

    doi: 10.1371/journal.pone.0174535

    Figure Lengend Snippet: SCNT embryos-derived exosomes were identified by immunofluorescence. The exosomes were bound to beads of a size that was in the detection range of the fluorescence microscope (4-μm diameter latex beads). The beads were then bound to fluorescence-conjugated antibody against CD9. Images were taken under epifluorescence (A, C, E) and DIC (B, D, F). A,B: embryos-derived exosomes; C,D: IgG negative control; E,F: embryo-free culture medium control. Bar, 10 μm.

    Article Snippet: After the embryos were washed thrice in 0.1% PBS-PVA for 5 min each wash, nuclear labeling was performed with 4,6-diamidino-2-phenylindole hydrochloride (DAPI, Vysis Inc., Downers Grove, USA) for 3 min. After wash and mounting, slides were examined by epifluorescence using a Nikon Eclipse Ti-S microscope (Nikon, Tokyo, Japan).

    Techniques: Derivative Assay, Immunofluorescence, Fluorescence, Microscopy, Negative Control

    Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted epifluorescence microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).

    Journal: Nutrition and cancer

    Article Title: Benzyl Isothiocyanate Inhibits HNSCC Cell Migration and Invasion, and Sensitizes HNSCC Cells to Cisplatin

    doi: 10.1080/01635581.2014.868912

    Figure Lengend Snippet: Vimentin expression in HN12 cells following one-hour treatment with a range of concentrations of BITC. (A) Representative immunofluorescence images of a vimentin immunostaining acquired with an inverted epifluorescence microscope before and after 1-hour treatment of HN12 cells with a range of concentrations of BITC (5–10μM). Anti-vimentin (1:400); AbII anti-rabbit Alexa Fluor 488 (1:200); DAPI to counterstain the nuclei. Magnification 400X. Bar size is 10μm. (B) Western blot analysis of vimentin expression in HN12 cells after 1-hour treatment with a range of concentrations of BITC (1.25–10μM). Actin was used to normalize the blot. (C) Densitometric analysis of vimentin and actin protein expression. Diagram represents the fold change of vimentin after 24 hours normalized to actin control. One-way ANOVA for multiple comparisons with Dunnett’s Post-Hoc test (* p ≤0.05).

    Article Snippet: Images taken using an Olympus IX51 inverted microscope equipped with epifluorescence (Olympus, Center Valley, PA).

    Techniques: Expressing, Immunofluorescence, Immunostaining, Inverted Epifluorescence, Western Blot

    BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an epifluorescence microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p

    Journal: Journal of Clinical Biochemistry and Nutrition

    Article Title: The preventive effect of Qing Dai on bisphosphonate-induced gastric cellular injuries

    doi: 10.3164/jcbn.17-108

    Figure Lengend Snippet: BP-induced cellular lipid peroxidation levels were evaluated by measuring DPPP-oxide fluorescence. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained using a chilled CCD camera mounted on an epifluorescence microscope (40×). B: The data shows the fluorescence intensity of DPPP (mean ± SD). The excitation and emission wavelengths are at 360 nm and 405 nm, respectively. n = 4, * p

    Article Snippet: Fluorescence images of cells were obtained with a chilled CCD camera mounted on an epifluorescence microscope (BZ-X710, KEYENCE, Osaka, Japan) with the excitation (ex.) and emission (em.) wavelengths at 360 and 405 nm, respectively.

    Techniques: Fluorescence, Microscopy

    BP-induced superoxide in cells was visualized with MitoSOX fluorescent dye and cellular mitochondria were observed with MitoTrackerGreen fluorescent dye. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained with a chilled CCD camera mounted on an epifluorescence microscope (60×). MitoSOX selectively accumulates in mitochondria and immediately reacts with superoxide, resulting in red fluorescence. MitoTrackerGreen enters mitochondria and shows green fluorescence. B: The data shows the fluorescence intensity of MitoSOX (mean ± SD). The excitation and emission wavelengths of MitoSOX are at 545 nm and 605 nm, respectively. The excitation and emission wavelengths of MitoTrackerGreen are at 470 nm and 525 nm, respectively. n = 4, * p

    Journal: Journal of Clinical Biochemistry and Nutrition

    Article Title: The preventive effect of Qing Dai on bisphosphonate-induced gastric cellular injuries

    doi: 10.3164/jcbn.17-108

    Figure Lengend Snippet: BP-induced superoxide in cells was visualized with MitoSOX fluorescent dye and cellular mitochondria were observed with MitoTrackerGreen fluorescent dye. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained with a chilled CCD camera mounted on an epifluorescence microscope (60×). MitoSOX selectively accumulates in mitochondria and immediately reacts with superoxide, resulting in red fluorescence. MitoTrackerGreen enters mitochondria and shows green fluorescence. B: The data shows the fluorescence intensity of MitoSOX (mean ± SD). The excitation and emission wavelengths of MitoSOX are at 545 nm and 605 nm, respectively. The excitation and emission wavelengths of MitoTrackerGreen are at 470 nm and 525 nm, respectively. n = 4, * p

    Article Snippet: Fluorescence images of cells were obtained with a chilled CCD camera mounted on an epifluorescence microscope (BZ-X710, KEYENCE, Osaka, Japan) with the excitation (ex.) and emission (em.) wavelengths at 360 and 405 nm, respectively.

    Techniques: Fluorescence, Microscopy

    Mitochondrial membrane potential of BP-treated cells was analyzed using MitoRed fluorescent reagent. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained with a chilled CCD camera mounted on an epifluorescence microscope (40×). B: The data show the fluorescence intensity of MitoRed (mean ± SD). The excitation and emission wavelengths of MitoRed are at 545 nm and 605 nm, respectively. n = 4, * p

    Journal: Journal of Clinical Biochemistry and Nutrition

    Article Title: The preventive effect of Qing Dai on bisphosphonate-induced gastric cellular injuries

    doi: 10.3164/jcbn.17-108

    Figure Lengend Snippet: Mitochondrial membrane potential of BP-treated cells was analyzed using MitoRed fluorescent reagent. RGM1 cells were pretreated with or without 5 µg/ml QD for 2.5 h and treated with 100 µM risedronate for 24 h. A: Fluorescence images were obtained with a chilled CCD camera mounted on an epifluorescence microscope (40×). B: The data show the fluorescence intensity of MitoRed (mean ± SD). The excitation and emission wavelengths of MitoRed are at 545 nm and 605 nm, respectively. n = 4, * p

    Article Snippet: Fluorescence images of cells were obtained with a chilled CCD camera mounted on an epifluorescence microscope (BZ-X710, KEYENCE, Osaka, Japan) with the excitation (ex.) and emission (em.) wavelengths at 360 and 405 nm, respectively.

    Techniques: Fluorescence, Microscopy