Journal: Advanced biosystems

Article Title: Matrix-Embedded Endothelial Cells Attain a Progenitor-Like Phenotype

doi: 10.1002/adbi.201700057

Figure Lengend Snippet: Expression of CD34 (an EPC marker) in ECs and EPCs as a function of time and substratum by FACS. A) Expression in MEECs and ME-EPCs at 3 d is as low as that of 2D-ECs and 2D-EPCs; however, expression increases in both cell types in 3D as a function of time. B) While mature ECs in the vessel wall have only ≈1% CD34+ cells,[18] MEECs 14 d in culture have a CD34 expression that matches and even surpasses that of ME-EPCs. Conversely, EPCs are highly CD34-expressing in vivo,[17] but lose this marker when cultured in 2D, resulting in expression levels that are comparable to 2D-ECs. n = 3; *p < 0.05.

Article Snippet: Cell Culture Primary human aortic ECs (HAECs, Lonza) and cord blood EPCs (Lonza) were isolated [ 17 ] and cultured in Endothelial Growth Medium-2 (EGM-2, Lonza) supplemented with an additional 8% (10% total) fetal bovine serum (FBS), penicillin (100 U mL −1 ) and streptomycin (100 μg mL −1 ), on gelatin-coated tissue culture polystyrene (gTCPS) plates (0.1% gelatin type A, Sigma, St. Louis, MO) and used between passages 2 and 6.

Techniques: Expressing, Marker, In Vivo, Cell Culture

Journal: Advanced biosystems

Article Title: Matrix-Embedded Endothelial Cells Attain a Progenitor-Like Phenotype

doi: 10.1002/adbi.201700057

Figure Lengend Snippet: Differential expression of endothelial biology genes as a function of substratum. A) Six genes are significantly upregulated in both ECs and EPCs in matrix-embedded culture compared to 2D. C) Fourteen further genes are upregulated specifically in MEECs compared to 2D-ECs. B,D) For each set of genes, the interaction maps show connections between these identified genes (circles) and neighboring downstream/upstream genes (diamonds). E) The 20 genes encompass a variety of endothelial functions. All genes shown were significantly upregulated (p < 0.05) by more than twofold after comparison of three samples per cell–substrate condition.

Article Snippet: Cell Culture Primary human aortic ECs (HAECs, Lonza) and cord blood EPCs (Lonza) were isolated [ 17 ] and cultured in Endothelial Growth Medium-2 (EGM-2, Lonza) supplemented with an additional 8% (10% total) fetal bovine serum (FBS), penicillin (100 U mL −1 ) and streptomycin (100 μg mL −1 ), on gelatin-coated tissue culture polystyrene (gTCPS) plates (0.1% gelatin type A, Sigma, St. Louis, MO) and used between passages 2 and 6.

Techniques: Expressing

Journal: Advanced biosystems

Article Title: Matrix-Embedded Endothelial Cells Attain a Progenitor-Like Phenotype

doi: 10.1002/adbi.201700057

Figure Lengend Snippet: Expression profile of endothelial biology genes in ME-EPCs compared to MEECs and 2D-ECs. A) While 14 genes had significantly increased expression in ME-EPCs compared to 2D-ECs, B) only 3 genes were upregulated in ME-EPCs compared to MEECs, C) with 2 of those common to both comparisons. D) Of the 14 genes differentially expressed between EPCs and 2D-ECs, 8 of these are indeed also seen when comparing MEECs and 2D-ECs. Unlike 2D-ECs, then MEECs attain a genetic fingerprint similar to ME-EPCs. E) The pathways of MAPK1, TGFB1, and HIF1A, which are central to this expression profile (Circles: genes probed; Diamonds: neighboring upstream/downstream genes). F) The genes covers a variety of functions. All genes shown were significantly upregulated (p < 0.05) by more than twofold after comparison of three samples per cell–substrate condition.

Article Snippet: Cell Culture Primary human aortic ECs (HAECs, Lonza) and cord blood EPCs (Lonza) were isolated [ 17 ] and cultured in Endothelial Growth Medium-2 (EGM-2, Lonza) supplemented with an additional 8% (10% total) fetal bovine serum (FBS), penicillin (100 U mL −1 ) and streptomycin (100 μg mL −1 ), on gelatin-coated tissue culture polystyrene (gTCPS) plates (0.1% gelatin type A, Sigma, St. Louis, MO) and used between passages 2 and 6.

Techniques: Expressing