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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models
doi: 10.1016/j.xpro.2024.103274
Figure Lengend Snippet:
Article Snippet:
Techniques: Immunofluorescence, Flow Cytometry, Control, Recombinant, Red Blood Cell Lysis, Saline, Software, Cell Culture, Plasmid Preparation, Microscopy
Journal: Journal of Breast Cancer
Article Title: The Presence of EpCAM - /CD49f + Cells in Breast Cancer Is Associated with a Poor Clinical Outcome
doi: 10.4048/jbc.2015.18.3.242
Figure Lengend Snippet: Dual-immunohistochemistry (IHC) and immunofluorescence (IF) staining on tissue microarray (TMA) slides. Dual-IHC and IF staining with primary antibodies of EpCAM and CD49f were performed on TMA tissue. (A) Patients only contain EpCAM-positive cancer cells (membrane); (B) Patient show only CD49f-positive cancer cells (cytoplasm); (C) Case express none or both (D) biomarkers. In IF staining (E-P), green signal shows the expression of CD49f, red signal shows EpCAM with DAPI staining for nuclei. (E-H) One case that tumor cell expression only EpCAM on the membrane (white arrows); (I-L) One case that tumor cell express only CD49f (red arrow); (M-P) One case with tumor cells express both (yellow arrow) (for both IHC and IF, magnification is actually ×400).
Article Snippet: IHC was performed using
Techniques: Immunohistochemistry, Immunofluorescence, Staining, Microarray, Membrane, Expressing
Journal: Journal of Breast Cancer
Article Title: The Presence of EpCAM - /CD49f + Cells in Breast Cancer Is Associated with a Poor Clinical Outcome
doi: 10.4048/jbc.2015.18.3.242
Figure Lengend Snippet: Prevalence of EpCAM - /CD49f + with histopathologic characteristics. Analyzing of 150 breast cancer samples stratified to tumor size (A), node status (B), tumor grade (C; low=grade I and II, high=grade III), recurrence (D), distant metastasis (E), and estrogen receptor (ER) expression (F). Tumor containing EpCAM - /CD49f + are associated with higher tumor grade (C; p =0.031) and distant metastasis (E; p =0.048). Chi-square exact and Fisher exact test were performed.
Article Snippet: IHC was performed using
Techniques: Expressing
Journal: Journal of Breast Cancer
Article Title: The Presence of EpCAM - /CD49f + Cells in Breast Cancer Is Associated with a Poor Clinical Outcome
doi: 10.4048/jbc.2015.18.3.242
Figure Lengend Snippet: Univariate survival analysis of EpCAM - /CD49f + with disease-free survival (DFS) and overall survival (OS). All the patients' tumor samples were stained with EpCAM and CD49f. (A, B) The present of EpCAM - /CD49f + tumor cells have significant negative association with both DFS and OS (EpCAM - /CD49f + : p DFS =0.009; p OS =0.001).
Article Snippet: IHC was performed using
Techniques: Staining
Journal: PLoS Pathogens
Article Title: Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment
doi: 10.1371/journal.ppat.1005641
Figure Lengend Snippet: ( A ) Cell entry is EpCAM dependent but independent of ephrin-B2. Representative flow cytometry plots out of three independent experiments of CHO-K1, CHO-EpCAM, CHO-ephrin-B2 cells, and of a mixed culture composed of CHO-EpCAM and CHO-ephrin-B2 (1:1 ratio) monitored 72 h after transduction with NiVmut EpCAM -LV, NiVwt-LV or VSV-LV (MOI of 1). EpCAM expression was detected by an APC-coupled human EpCAM specific antibody. ( B ) To ascertain stability of transduction with the EpCAM-targeted vector, CHO-EpCAM cells were cultivated for further 30 days after transduction with the indicated MOIs. The percentage of GFP-positive cells was determined by flow cytometry at the indicated time points. One representative out of three independent experiments is shown.
Article Snippet: Human EpCAM was detected by an Allophycocyanin (APC) labeled
Techniques: Flow Cytometry, Transduction, Expressing, Plasmid Preparation