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Image Search Results
Journal: Integrative Biology
Article Title: Rapid translocation of pluripotency-related transcription factors by external uniaxial forces
doi: 10.1093/intbio/zyz003
Figure Lengend Snippet: Molecular characterization and identification of 10% strain applied human ESCs for 2 h via qRT-PCR analysis for temporal expression of (A) pluripotency (NANOG, OCT4, SOX2, and KLF4), (B) trophoctoderm markers (CDX2, EOMES, EpCAM, and FGF4), (C) ecto- and endodermal lineage differentiation (WNT3A, SOX17, GATA6, and PAX6), and (D) cytoskeletal, focal adhesion kinase (Ptk2) and PXN (Paxillin), Integrin alpha 6 (ITGA6) and E-CADHERIN with and without 10% strain. All quantification from three independent replicates. Unpaired t test P values <0.05 (*), <0.01 (**), <0.001(***), n.s.: not significant.
Article Snippet: All primers were purchased from ThermoFisher Life Technologies. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Gene symbol Assay ID UniGene ID NANOG Hs02387400_g1 Hs.635882 POU5F1 (OCT 3/4) Hs03005111_g1 (FAM-MGB) Hs.249184 SOX2 Hs01053049 (FAM-MGB) Hs.518438 FAK (PTK2) Hs03657683 Hs.395482 CDX2 Hs01078080_m1 Hs.127383 E-CADHERIN Hs01023894_m1 Hs.461086 PXN Hs01104424_m1 Hs.446336 EOMES
Techniques: Quantitative RT-PCR, Expressing
Journal: Integrative Biology
Article Title: Rapid translocation of pluripotency-related transcription factors by external uniaxial forces
doi: 10.1093/intbio/zyz003
Figure Lengend Snippet: List of primary antibodies.
Article Snippet: All primers were purchased from ThermoFisher Life Technologies. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Gene symbol Assay ID UniGene ID NANOG Hs02387400_g1 Hs.635882 POU5F1 (OCT 3/4) Hs03005111_g1 (FAM-MGB) Hs.249184 SOX2 Hs01053049 (FAM-MGB) Hs.518438 FAK (PTK2) Hs03657683 Hs.395482 CDX2 Hs01078080_m1 Hs.127383 E-CADHERIN Hs01023894_m1 Hs.461086 PXN Hs01104424_m1 Hs.446336 EOMES
Techniques:
Journal: Integrative Biology
Article Title: Rapid translocation of pluripotency-related transcription factors by external uniaxial forces
doi: 10.1093/intbio/zyz003
Figure Lengend Snippet: Primer sets used in qRT-PCR
Article Snippet: All primers were purchased from ThermoFisher Life Technologies. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Gene symbol Assay ID UniGene ID NANOG Hs02387400_g1 Hs.635882 POU5F1 (OCT 3/4) Hs03005111_g1 (FAM-MGB) Hs.249184 SOX2 Hs01053049 (FAM-MGB) Hs.518438 FAK (PTK2) Hs03657683 Hs.395482 CDX2 Hs01078080_m1 Hs.127383 E-CADHERIN Hs01023894_m1 Hs.461086 PXN Hs01104424_m1 Hs.446336 EOMES
Techniques: