enzyme moloney murine leukemia virus reverse transcriptase Search Results


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  • 99
    Thermo Fisher m mulv reverse transcriptase
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore avian rt first strand synthesis kit
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    Avian Rt First Strand Synthesis Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher revertaid m mulv reverse transcriptase
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    Revertaid M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1914 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 1914 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher revertaid h minus m mulv reverse transcriptase
    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with <t>Moloney</t> murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.
    Revertaid H Minus M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1495 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore moloney murine leukemia virus reverse transcriptase
    Strategy for ablation of NF-YA exon 3 in C2C12 cells using CRISPR/Cas9n and four gRNAs. ( A ) Gene editing strategy for NF-YA exon 3 deletion using the Cas9-nickase (Cas9n) and four guide RNAs. The targeted sequence by each guide <t>RNA</t> and the deletion sites are shown. Note that Cas9n cuts only the DNA strand that is complementary to and recognized by the gRNA, making necessary the simultaneous presence of two gRNAs/Cas9n complexes to induce a double-strand break (DSB). ( B ) The three primer pairs used to check for positive C2-YAl-KO clones are shown with the specific amplification products highlighted by the dashed lines. ( C ) Example of <t>PCR</t> products run into a 1.2% agarose gel. The expected bands in control cells (ctr) are marked with arrowheads; clones #83 and #117 represent positive C2-YAl-KO clones.
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher m mulv reverse transcriptase rnase h
    Strategy for ablation of NF-YA exon 3 in C2C12 cells using CRISPR/Cas9n and four gRNAs. ( A ) Gene editing strategy for NF-YA exon 3 deletion using the Cas9-nickase (Cas9n) and four guide RNAs. The targeted sequence by each guide <t>RNA</t> and the deletion sites are shown. Note that Cas9n cuts only the DNA strand that is complementary to and recognized by the gRNA, making necessary the simultaneous presence of two gRNAs/Cas9n complexes to induce a double-strand break (DSB). ( B ) The three primer pairs used to check for positive C2-YAl-KO clones are shown with the specific amplification products highlighted by the dashed lines. ( C ) Example of <t>PCR</t> products run into a 1.2% agarose gel. The expected bands in control cells (ctr) are marked with arrowheads; clones #83 and #117 represent positive C2-YAl-KO clones.
    M Mulv Reverse Transcriptase Rnase H, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega moloney murine leukemia virus reverse transcriptase enzyme
    Strategy for ablation of NF-YA exon 3 in C2C12 cells using CRISPR/Cas9n and four gRNAs. ( A ) Gene editing strategy for NF-YA exon 3 deletion using the Cas9-nickase (Cas9n) and four guide RNAs. The targeted sequence by each guide <t>RNA</t> and the deletion sites are shown. Note that Cas9n cuts only the DNA strand that is complementary to and recognized by the gRNA, making necessary the simultaneous presence of two gRNAs/Cas9n complexes to induce a double-strand break (DSB). ( B ) The three primer pairs used to check for positive C2-YAl-KO clones are shown with the specific amplification products highlighted by the dashed lines. ( C ) Example of <t>PCR</t> products run into a 1.2% agarose gel. The expected bands in control cells (ctr) are marked with arrowheads; clones #83 and #117 represent positive C2-YAl-KO clones.
    Moloney Murine Leukemia Virus Reverse Transcriptase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with Moloney murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.

    Journal: Infection and Immunity

    Article Title: Phagocytosis of the Malarial Pigment, Hemozoin, Impairs Expression of Major Histocompatibility Complex Class II Antigen, CD54, and CD11c in Human Monocytes

    doi:

    Figure Lengend Snippet: Quantification of MHC class I and class II mRNA by RT-PCR. Increasing amounts (7.5 to 60 ng, quantified by OD, as indicated under the lanes) of total RNA extracted from human monocytes were employed in reverse transcription with Moloney murine leukemia virus reverse transcriptase for 1 h, followed by PCR in the same tube (100-μl final volume, 25 cycles). RT-PCR was performed in duplicate with each of the indicated total RNA amounts. Specific primers for conserved regions in MHC class I and class II genes were utilized to obtain PCR products of 118 and 81 bp, respectively. PCR products were separated on a 3% agarose gel and stained with ethidium bromide. DNA size standards are indicated on the right.

    Article Snippet: The cDNA synthesis from 15 ng of total cellular RNA from each extract was performed with 25 ng of random primers (Gibco BRL), 200 U of Moloney murine leukemia virus reverse transcriptase (Gibco BRL), and 20 U of RNase inhibitor (Boehringer Mannheim).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining

    Strategy for ablation of NF-YA exon 3 in C2C12 cells using CRISPR/Cas9n and four gRNAs. ( A ) Gene editing strategy for NF-YA exon 3 deletion using the Cas9-nickase (Cas9n) and four guide RNAs. The targeted sequence by each guide RNA and the deletion sites are shown. Note that Cas9n cuts only the DNA strand that is complementary to and recognized by the gRNA, making necessary the simultaneous presence of two gRNAs/Cas9n complexes to induce a double-strand break (DSB). ( B ) The three primer pairs used to check for positive C2-YAl-KO clones are shown with the specific amplification products highlighted by the dashed lines. ( C ) Example of PCR products run into a 1.2% agarose gel. The expected bands in control cells (ctr) are marked with arrowheads; clones #83 and #117 represent positive C2-YAl-KO clones.

    Journal: Cells

    Article Title: The Switch from NF-YAl to NF-YAs Isoform Impairs Myotubes Formation

    doi: 10.3390/cells9030789

    Figure Lengend Snippet: Strategy for ablation of NF-YA exon 3 in C2C12 cells using CRISPR/Cas9n and four gRNAs. ( A ) Gene editing strategy for NF-YA exon 3 deletion using the Cas9-nickase (Cas9n) and four guide RNAs. The targeted sequence by each guide RNA and the deletion sites are shown. Note that Cas9n cuts only the DNA strand that is complementary to and recognized by the gRNA, making necessary the simultaneous presence of two gRNAs/Cas9n complexes to induce a double-strand break (DSB). ( B ) The three primer pairs used to check for positive C2-YAl-KO clones are shown with the specific amplification products highlighted by the dashed lines. ( C ) Example of PCR products run into a 1.2% agarose gel. The expected bands in control cells (ctr) are marked with arrowheads; clones #83 and #117 represent positive C2-YAl-KO clones.

    Article Snippet: Reverse Transcriptase PCR and Real-Time PCR RNA was isolated by the Tri Reagent (Sigma-Aldrich) protocol according to the manufacturer’s instruction.

    Techniques: CRISPR, Sequencing, Clone Assay, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis