Journal: PeerJ

Article Title: Development of a multiplex qPCR assay for the simultaneous detection of Mycoplasma bovis, Mycoplasma species, and Acholeplasma laidlawii in milk

doi: 10.7717/peerj.11881

Figure Lengend Snippet: Bacterial and algal strains used in this study.

Article Snippet: Enterobacter cloacae subsp cloacae , ATCC 13847.

Techniques:

Journal: Microorganisms

Article Title: Whole Genome Sequencing Based Taxonomic Classification, and Comparative Genomic Analysis of Potentially Human Pathogenic Enterobacter spp. Isolated from Chlorinated Wastewater in the North West Province, South Africa

doi: 10.3390/microorganisms9091928

Figure Lengend Snippet: Trees inferred with FastME 2.1.6.1 from the Genome Blast Distance Phylogeny (GBDP) distances calculated from whole-genome sequences. Isolates S4 ( A ) and S5 ( B ) form a species cluster with Enterobacter hormaechei subsp. hoffmannii, having a dDDH of >70%, a clear indication that the isolates belong to the same species as E. hormaechei subsp. hoffmannii .

Article Snippet: S5SPAdes.contigs.fa , Enterobacter cloacae subsp. dissolvens ATCC 23373 T , 32.1 , [29.7–34.6] , 0.18.

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Journal: Microorganisms

Article Title: Whole Genome Sequencing Based Taxonomic Classification, and Comparative Genomic Analysis of Potentially Human Pathogenic Enterobacter spp. Isolated from Chlorinated Wastewater in the North West Province, South Africa

doi: 10.3390/microorganisms9091928

Figure Lengend Snippet: A heatmap showing clusters of orthologous genes (COGs) for isolates S4, S5, and a representative of all validly published Enterobacter species (see Materials and Methods for full species list and accession numbers). The COGs covered cellular processing and signaling (D: cell cycle control, cell division, chromosome partitioning; M: cell wall/membrane/envelope biogenesis; N: cell motility; O: post-translational modification, protein turnover, and chaperones; T: signal transduction mechanism; U: intracellular trafficking, secretion, and vesicular transportation; V: defense mechanisms; W: extracellular structures; Z: cytoskeleton), information storage and processing (A: RNA processing and modification; J: translation, ribosomal structure, and biogenesis; K: transportation; L: replication, recombination, and repair), metabolism (C: energy production and conversion; E: amino acid transport and metabolism; F: nucleotide transport and metabolism; G: carbohydrate transport and metabolism; H: coenzyme transport and metabolism; I: lipid transport and metabolism; P: inorganic ion transport and metabolism; Q: secondary metabolites biosynthesis, transport, and catabolism), as well as the poorly characterized (R and S).

Article Snippet: S5SPAdes.contigs.fa , Enterobacter cloacae subsp. dissolvens ATCC 23373 T , 32.1 , [29.7–34.6] , 0.18.

Techniques: Control, Membrane, Modification, Transduction

Journal: Microorganisms

Article Title: Whole Genome Sequencing Based Taxonomic Classification, and Comparative Genomic Analysis of Potentially Human Pathogenic Enterobacter spp. Isolated from Chlorinated Wastewater in the North West Province, South Africa

doi: 10.3390/microorganisms9091928

Figure Lengend Snippet: Pan-genome circular visualization output. ( A ) Isolates S4 and S5 compared to representatives of all validly published Enterobacter species (see Materials and Methods for full species list and accession numbers); each red strip indicates a gene that is unique to the isolate S5, which was used as a base genome. ( B ) Isolate S5 compared to validly published Enterobacter species; each red strip indicates a gene that is unique to the isolate S5. ( B ) Isolate S4 is not included in the analysis as it is highly identical to isolate S5 and would mask the true variation in the isolates compared to other Enterobacter species.

Article Snippet: S5SPAdes.contigs.fa , Enterobacter cloacae subsp. dissolvens ATCC 23373 T , 32.1 , [29.7–34.6] , 0.18.

Techniques: Stripping Membranes

Journal: Microorganisms

Article Title: Whole Genome Sequencing Based Taxonomic Classification, and Comparative Genomic Analysis of Potentially Human Pathogenic Enterobacter spp. Isolated from Chlorinated Wastewater in the North West Province, South Africa

doi: 10.3390/microorganisms9091928

Figure Lengend Snippet: Distribution of a selected SEED domain categories between the isolates (S4, S5) and the representatives of all validly published Enterobacter species (see Materials and Methods for full species list and accession numbers). Interestingly, isolates S4 and S5 have the largest proportion of Type 4 Secretion System in addition to being the only genomes carrying Tn522.

Article Snippet: S5SPAdes.contigs.fa , Enterobacter cloacae subsp. dissolvens ATCC 23373 T , 32.1 , [29.7–34.6] , 0.18.

Techniques:

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

Article Title: High Throughput Screening and QSAR-3D/CoMFA: Useful Tools to Design Predictive Models of Substrate Specificity for Biocatalysts †

doi: 10.3390/90800673

Figure Lengend Snippet: Reduction of cyclohexanone. Microorganisms that afford cyclohexanol yields greater than 50%. [Cyclohexanone] = 10 mM. Reaction time = 72 h. T = 28 ºC. Stirring speed = 250 rpm. [ 24 ]

Article Snippet: Therefore, we selected microorganisms that do not give secondary reactions and that gave more than a 50% yield of cyclohexanol. table ft1 table-wrap mode="anchored" t5 caption a7 Entry Phylum Microorganisms References Reduction Yield (%) 1 Filamentous Fungus Gongronella butleri CBS 157.25 97 2 Marine Fungus Zopfiella latipes ATCC 26183 96 3 Basidiomycete Coriolus azureus CBS 410.66 96 4 Filamentous Fungus Monascus kaoliang CBS 302.78 89 5 Marine Fungus Buergenerula spartinae ATCC 62545 88 6 Yeast Schizosaccharomyces octosporus NCYC 427 87 7 Filamentous Fungus Diplogelasinospora grovesii IMI 171018 85 8 Yeast Issatchenkia scutulata CBS 6670 81 9 Filamentous Fungus Absidia glauca CBS 100.48 81 10 Marine Fungus Dactylospora haliotrepha ATCC 66950 78 11 Filamentous Fungus Rhizomucor variabilis CBS 384.95 78 12 Filamentous Fungus Neosartorya hiratsukae CBS 294.93 71 13 Yeast Sirobasidium magnum CBS 6803 69 14 Filamentous Fungi Pyrenochaeta oryzae IMI 195679 67 15 Yeast Schwanniomyces occidentalis NCYC 133 66 16 Filamentous Fungus Actinomucor elegans CBS 100.09 66 17 Filamentous Fungus Neosartorya aureola CBS 105.55 65 18 Yeast ] Filobasidium capsuligenum ] NCYC 606 65 19 Yeast Trichosporon aquatile NCYC 2635 64 20 Filamentous Fungus Marssonina brunnea .

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