Journal: Scientific Reports

Article Title: Phosphatidylcholine synthesis through cholinephosphate cytidylyltransferase is dispensable in Leishmania major

doi: 10.1038/s41598-019-44086-6

Figure Lengend Snippet: Cpct − mutants show significant growth delay when co-cultured with bacteria and the growth delay can be rescued by EtN supplementation. Effects of Serratia marcescens and Enterobacter cloacae on the growth of cpct − mutants were determined in a co-culture experiment. L . major promastigotes (1 × 10 6 cells/ml) were incubated in lipid-free M199 medium alone or with Serratia marcescens (50 bacteria/ml) or Enterobacter cloacae (50 bacteria/ml). EtN (250 μM) was included as indicated. The number of promastigotes/ml was recorded using a hemocytometer after 24 h. Results were averaged from three independent experiments with triplicates. Error bars represent standard deviations from three biological repeats (* p < 0.05; *** p < 0.001) based on one way Anova relative to WT.

Article Snippet: Serratia marcescens (ATCC #13880) and Enterobacter cloacae (Carolina Biological # 155032) were cultured in LB media at 37 °C until OD 600 nm reached 0.6.

Techniques: Cell Culture, Bacteria, Co-Culture Assay, Incubation