Journal: bioRxiv

Article Title: Human SHED-derived extracellular cues activate a specialized neuroprotective and regenerative program in developing retinal ganglion cells

doi: 10.64898/2026.06.25.733625

Figure Lengend Snippet: (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.

Article Snippet: Antibodies against the following human antigens were used: CD105-FITC (Miltenyi Biotect, Bergisch Gladbach, Germany, cat# 130-112-327, 1:50), CD90-FITC (Miltenyi Biotec, cat# 130-114-901, 1:50), CD44-VioBlue (Miltenyi Biotec, cat# 130-113-906, 1:50), CD73-APC (Miltenyi Biotec, cat# 130-111-909, 1:50), MSC Phenotyping Cocktail-PE (CD34, CD14, CD19, CD45, Miltenyi Biotec cat# 130-125-285, dilution according to the manufacturer’s instructions).

Techniques: Immunofluorescence, Staining, Expressing, Immunostaining, Flow Cytometry, Derivative Assay

Journal: Cell Regeneration

Article Title: Discovery of a chemical small molecule inducing umbilical cord mesenchymal stem cell differentiation to vascular endothelial cells

doi: 10.1186/s13619-025-00278-2

Figure Lengend Snippet: CPP Induces Phenotypic Remodeling of UC-MSCs. A Chemical structure of CPP. B hUC -MSCs were continuously induced by different concentrations of CPP (0, 0.5, 1, 2, 5, 10 μM) for 120 h and observed continuously using an inverted phase contrast microscope. Scale bar: 50 μm. C hUC-MSCs were treated with different concentrations of CPP (0, 1, 2, 5, 10 μM) for 48, 72, 96, and 120 h, and the changes in CD44 protein levels were detected by Western blot. D Quantification of CD44 Western blot bands using GAPDH as the loading control. E hUC-MSCs were treated with different concentrations of CPP (0, 1, 2, 5, 10 μM) for 48, 72, 96, and 120 h, and the changes in CD105 protein levels were detected by Western blot. F Quantification of CD105 Western blot bands using GAPDH as the loading control

Article Snippet: Antibodies against CD44 (A19020), CD105 (A19008), CD133 (A0818), CD31 (A2104) were from ABclonal Technology.

Techniques: Microscopy, Western Blot, Control

Journal: Journal of cancer research and therapeutics

Article Title: Carboxylesterase-overexpressing hTERT-immortalized human adipose stem cells in prostate tumor growth inhibition by irinotecan.

doi: 10.4103/jcrt.jcrt_1019_21

Figure Lengend Snippet: Figure 2: Cell markers for hTERT‑hADSC.GFP and hTERT‑hADSC. GFP.CE cells. hTERT‑ADSC cells express cell type‑specific markers for mesenchymal stem cell markers CD29, CD90, and CD105, but not for hematopoietic stem cell CD34 or CD45. hTERT‑hADSC. CE = CE overexpressing hTERT immortalized human adipose stem cells. ADCS = hTERT‑hADSC, ADSC.CE = CE‑overexpressing hTERT immortalized human adipose stem cells, hTERT‑hADSC. CE = CE‑overexpressing hTERT‑immortalized human adipose‑derived stem cell line, CE = Carboxylesterase

Article Snippet: The hTERT‐ADSC and hTERT‐ADSC.CE were subjected to flow cytometry analysis using anti‐CD29 (1:50, Origene Technologies, Rockville, MD, USA), CD34 (1:50, Invitrogen, Carlsbad, CA, USA), CD45 (1:50, LDBio, Seattle, WA, USA), CD90 (1:50, Origene Technologies), and CD105 (1:50, Origene Technologies) antibodies.

Techniques:

Journal: BMC Genomics

Article Title: Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenic development

doi: 10.1186/1471-2164-12-461

Figure Lengend Snippet: Morphology and surface marker presentation of human MSC (A-C) . Human mesenchymal stem cells (MSC) were isolated from bone marrow aspirates. During primary culture (A) and up to passage 3 (B) they showed a typical fibroblast-like morphology and grew in a swirling-like pattern. Flow cytometry analysis demonstrated a homogenous MSC population (C). As expected, MSC were negative for reactivity to antigens CD14, CD34 and CD45, but positive for reactivity to antigens CD44, CD73, CD90, CD105 and CD166.

Article Snippet: Monoclonal CD105 was purchased from Acris Antibodies (Hiddenhausen, Germany).

Techniques: Marker, Isolation, Flow Cytometry

Journal: BMC Genomics

Article Title: Differential gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenic development

doi: 10.1186/1471-2164-12-461

Figure Lengend Snippet: Control of adipocyte differentiation by FACS studies . The adipocytic differentiation was also monitored using flow cytometry with different markers. After growing 14 days some of the cells were harvested and marked with the monoclonal antibodies CD26, CD29, CD49b, CD55, CD73, CD90, CD140a (all Becton Dickinson, San Jose, CA), CD49f, CD120a (both Serotec, Raleigh, NC) and CD105 (Caltag, Buckingham, UK). As a negative control, cells were incubated with the corresponding irrelevant mouse IgG1, IgG2a, IgG2b or IgGM mAbs - therefore, cells became visible in the background. But only if a gene expression is changing in relation to the background after 14 days, can the result be positive. In Figure 4 the cells were colored early in the beginning with a blue marker and after 14 days with a red coloration. Only mAb CD140a clearly showed an increased gene expression after 14 days.

Article Snippet: Monoclonal CD105 was purchased from Acris Antibodies (Hiddenhausen, Germany).

Techniques: Control, Flow Cytometry, Bioprocessing, Negative Control, Incubation, Gene Expression, Marker